Issue 73, 2013
From the journal:

Chemical Communications

High-throughput and long-term observation of compartmentalized biochemical oscillators†

Koshi Hasatani,a   Mathieu Leocmach,b   Anthony J. Genot,a   André Estévez-Torres,c   Teruo Fujiia  and  Yannick Rondelez*a  

* Corresponding authors

a LIMMS/CNRS-IIS, University of Tokyo, Komaba 4-6-2 Meguro-ku, Tokyo, Japan
E-mail: rondelez@iis.u-tokyo.ac.jp

b Laboratoire de Physique, UMR 5672, Ecole Normale Supérieure de Lyon - site Monod, 46 allée d'Italie, 69364 Lyon cedex 07, France

c Laboratoire de photonique et de nanostructures, CNRS, route de Nozay, 91460 Marcoussis, France

Abstract

We report the splitting of an oscillating DNA circuit into ∼700 droplets with picoliter volumes. Upon incubation at constant temperature, the droplets display sustained oscillations that can be observed for more than a day. Superimposed to the bulk behaviour, we find two intriguing new phenomena – slow desynchronization between the compartments and kinematic spatial waves – and investigate their possible origin. This approach provides a route to study the influence of small volume effects in biology, and paves the way to technological applications of compartmentalized molecular programs controlling complex dynamics.

Graphical abstract: High-throughput and long-term observation of compartmentalized biochemical oscillators

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Article information

DOI
https://doi.org/10.1039/C3CC44323J
Article type
Communication
Submitted
08 Jun 2013
Accepted
20 Jun 2013
First published
05 Aug 2013

Chem. Commun., 2013,49, 8090-8092

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High-throughput and long-term observation of compartmentalized biochemical oscillators

K. Hasatani, M. Leocmach, A. J. Genot, A. Estévez-Torres, T. Fujii and Y. Rondelez, Chem. Commun., 2013, 49, 8090 DOI: 10.1039/C3CC44323J

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