A far-red fluorescent probe based on a phospha-fluorescein scaffold for cytosolic calcium imaging

Hiroaki Ogasawara; Marek Grzybowski; Riho Hosokawa; Yoshikatsu Sato; Masayasu Taki; Shigehiro Yamaguchi

doi:10.1039/C7CC07344E

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A far-red fluorescent probe based on a phospha-fluorescein scaffold for cytosolic calcium imaging†

Hiroaki Ogasawara,

^a Marek Grzybowski,

^b Riho Hosokawa,^a Yoshikatsu Sato,^b Masayasu Taki

*^bc and Shigehiro Yamaguchi

*^ab

Author affiliations

* Corresponding authors

^a Department of Chemistry, Graduate School of Science and Integrated Research Consortium on Chemical Sciences (IRCCS), Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan

^b Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Furo, Chikusa, Nagoya 464-8601, Japan
E-mail: taki@itbm.nagoya-u.ac.jp, yamaguchi@chem.nagoya-u.ac.jp

^c JST-PRESTO, 4-1-8, Honcho, Kawaguchi, Saitama 332-0012, Japan

Abstract

The far-red emissive fluorescent probe CaPF-1 based on a phospha-fluorescein scaffold enables the detection of cytosolic calcium ions in living cells. The probe can be excited in the red region (λ_abs = 636 nm) and exhibits a sufficiently high fluorescence turn-on response in the far-red region (λ_em = 663 nm) upon complexation with calcium ions. The hydrophilic and anionic characteristics of this phospha-fluorescein fluorophore allowed the cytosolic localization of CaPF-1. Moreover, it was possible to visualize histamine-induced calcium oscillation in HeLa cells using CaPF-1.

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DOI: https://doi.org/10.1039/C7CC07344E
Article type: Communication
Submitted: 20 Sep 2017
Accepted: 06 Dec 2017
First published: 07 Dec 2017

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Chem. Commun., 2018,54, 299-302

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A far-red fluorescent probe based on a phospha-fluorescein scaffold for cytosolic calcium imaging

H. Ogasawara, M. Grzybowski, R. Hosokawa, Y. Sato, M. Taki and S. Yamaguchi, Chem. Commun., 2018, 54, 299 DOI: 10.1039/C7CC07344E

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Chemical Communications

A far-red fluorescent probe based on a phospha-fluorescein scaffold for cytosolic calcium imaging†

Abstract

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A far-red fluorescent probe based on a phospha-fluorescein scaffold for cytosolic calcium imaging

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