Precision improvement in dark-field microscopy imaging by using gold nanoparticles as an internal reference: a combined theoretical and experimental study

Jun Ma; Yue Liu; Peng Fei Gao; Hong Yan Zou; Cheng Zhi Huang

doi:10.1039/C5NR08837B

Precision improvement in dark-field microscopy imaging by using gold nanoparticles as an internal reference: a combined theoretical and experimental study†

Jun Ma,^a Yue Liu,^a Peng Fei Gao,^b Hong Yan Zou^b and Cheng Zhi Huang*^ab

* Corresponding authors

^a Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China
E-mail: chengzhi@swu.edu.cn
Fax: +86 23 68367257
Tel: +86 23 68254659

^b Chongqing Key Laboratory of Biomedical Analysis (Southwest University), Chongqing Science & Technology Commission, College of Pharmaceutical Science, Southwest University, Chongqing 400716, China

Abstract

Low accuracy is a big obstacle in the dark-field microscopy imaging (iDFM) technique in practical applications. In order to reduce the deviations and fluctuations in the observed or snapped scattered light in the iDFM technique caused by unavoidable measurement errors, bare gold nanoparticles (AuNPs) were introduced as an internal reference (IR). The feasibility of using AuNPs as the IR in iDFM in theory was verified. The function of the IR in improving the precision of the acquired data through post data analysis was identified by three kinds of experiments: monitoring the oxidation process of silver nanoparticles (AgNPs) at room temperature, quantifying the level of glucose with AgNPs used as probes and quantifying the change in the light intensity of AuNPs after the plasmon resonance energy transfer (PRET) between AuNPs and tetramethylrhodamine (TAMRA).

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DOI: https://doi.org/10.1039/C5NR08837B
Article type: Paper
Submitted: 12 Dec 2015
Accepted: 10 Mar 2016
First published: 11 Mar 2016

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Nanoscale, 2016,8, 8729-8736

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Precision improvement in dark-field microscopy imaging by using gold nanoparticles as an internal reference: a combined theoretical and experimental study

J. Ma, Y. Liu, P. F. Gao, H. Y. Zou and C. Z. Huang, Nanoscale, 2016, 8, 8729 DOI: 10.1039/C5NR08837B

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Precision improvement in dark-field microscopy imaging by using gold nanoparticles as an internal reference: a combined theoretical and experimental study†

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Precision improvement in dark-field microscopy imaging by using gold nanoparticles as an internal reference: a combined theoretical and experimental study

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