Vilas, J.L. et al. Structure 26, 337–344 (2018).

Resolution is perhaps the most important parameter for judging the quality of an electron density map determined by cryo-EM. The Fourier shell correlation (FSC) is the most common metric for estimating global resolution, but this approach cannot account for variance in the local resolution of different areas of the cryo-EM map. ResMap is the current state-of-the-art tool for estimating local resolution; although powerful, the method has several limitations. Vilas et al. address these limitations with their new tool MonoRes. An appealing and unique feature of MonoRes is that it produces a 3D resolution map; it is also fast and fully automated. The authors benchmarked its performance on both simulated and experimental cryo-EM maps, in comparison with ResMap and other tools. MonoRes is available via the Xmipp and Scipion software packages.