Resolution is perhaps the most important parameter for judging the quality of an electron density map determined by cryo-EM. The Fourier shell correlation (FSC) is the most common metric for estimating global resolution, but this approach cannot account for variance in the local resolution of different areas of the cryo-EM map. ResMap is the current state-of-the-art tool for estimating local resolution; although powerful, the method has several limitations. Vilas et al. address these limitations with their new tool MonoRes. An appealing and unique feature of MonoRes is that it produces a 3D resolution map; it is also fast and fully automated. The authors benchmarked its performance on both simulated and experimental cryo-EM maps, in comparison with ResMap and other tools. MonoRes is available via the Xmipp and Scipion software packages.
Rights and permissions
About this article
Cite this article
Local resolution of cryo-EM maps with MonoRes. Nat Methods 15, 246 (2018). https://doi.org/10.1038/nmeth.4651
Published:
Issue Date:
DOI: https://doi.org/10.1038/nmeth.4651
This article is cited by
-
Measurement of atom resolvability in cryo-EM maps with Q-scores
Nature Methods (2020)