This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
-
Self Fourier shell correlation: properties and application to cryo-ET
Communications Biology Open Access 16 January 2024
-
Structural basis for DNA proofreading
Nature Communications Open Access 27 December 2023
-
Cryo-EM structures of Aβ40 filaments from the leptomeninges of individuals with Alzheimer’s disease and cerebral amyloid angiopathy
Acta Neuropathologica Communications Open Access 04 December 2023
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
References
Henderson, R. et al. Structure 20, 205–214 (2012).
Saxton, W.O. & Baumeister, W. J. Microsc. 127, 127–138 (1982).
Stewart, A. & Grigorieff, N. Ultramicroscopy 102, 67–84 (2004).
Scheres, S.H. J. Mol. Biol. 415, 406–418 (2012).
Henderson, R. et al. J. Mol. Biol. 413, 1028–1046 (2011).
Scheres, S.H. et al. Nat. Protoc. 3, 977–990 (2008).
Böttcher, B., Wynne, S.A. & Crowther, R.A. Nature 386, 88–91 (1997).
Rosenthal, P.B. & Henderson, R. J. Mol. Biol. 333, 721–745 (2003).
Acknowledgements
We are grateful to T. Crowther and R. Henderson for helpful discussions and to J. Grimmett for help with computing. T. Crowther provided hepatitis B data, and the National Center for Macromolecular Imaging, which is funded by US National Institutes of Health grant P41RR02250, provided GroEL data. This work was funded by the UK Medical Research Council through grant MC_UP_A025_1013 to S.H.W.S.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Supplementary information
Supplementary Text and Figures
Supplementary Figures 1–4, Supplementary Table 1, Supplementary Methods and Supplementary Software (PDF 765 kb)
Rights and permissions
About this article
Cite this article
Scheres, S., Chen, S. Prevention of overfitting in cryo-EM structure determination. Nat Methods 9, 853–854 (2012). https://doi.org/10.1038/nmeth.2115
Published:
Issue Date:
DOI: https://doi.org/10.1038/nmeth.2115
This article is cited by
-
Structure of GPR101–Gs enables identification of ligands with rejuvenating potential
Nature Chemical Biology (2024)
-
Self Fourier shell correlation: properties and application to cryo-ET
Communications Biology (2024)
-
Identical tau filaments in subacute sclerosing panencephalitis and chronic traumatic encephalopathy
Acta Neuropathologica Communications (2023)
-
Cryo-EM structures of Aβ40 filaments from the leptomeninges of individuals with Alzheimer’s disease and cerebral amyloid angiopathy
Acta Neuropathologica Communications (2023)
-
Cryo-EM structures of tau filaments from the brains of mice transgenic for human mutant P301S Tau
Acta Neuropathologica Communications (2023)