Abstract
The objective of this study was to evaluate the role of2,6-dichlorophenol (2,6-DCP) as sex pheromone of Anocentornitens. Sex pheromones were extracted by submerging 150 females fed for 6 days in hexane (3 ml) for two hours and sonicating them for15 min. The extract was analyzed by GC-MS employing the Single Ion Monitoring (SIM) method. Identification of 2,6-DCP was based on the comparison of mass spectra with a computer search using the NIST library and by matching the mass spectrum of the peak at the retention time of 2,6-DCP from extract sand the synthetic product. In an olfactometer, males were released at 2.5cm from females, control rubber septa and impregnated rubber septa with increasing concentrations of 2,6-DCP (50, 500 and 5000 ng). A higher percentage of orientation and higher frequencies of angles between 0° and 10° were observed for males tested with females than with controls. Attraction of males to dummies impregnated with 2,6-DCP was observed with highest response (100%) at 50 ng. There was a gradual decrease of responsiveness of males with increasing concentration of 2,6-DCP (70 to 75%).This compound stimulated a behavior of mounting and ventral positioning ofA. nitens males at the lowest 2,6-DCP concentration where as dummies impregnated with 2,6-DCP higher than 50 ng concentration inhibited these behaviors. Therefore, we can conclude that 2,6-DCP can elicit the complete behavior sequence of orientation, location, mounting and ventral positioning behaviors and plays a role as an attractant and, as a mounting sex pheromone in A. nitens.
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References
Berger R.S. 1972. 2,6-dichlorophenol, sex pheromone of the lone star tick. Science 177: 704-705.
Borges L.M.F. and Ribeiro M.F.B. 1999. Presence of sex pheromones in Anocentor nitens (Acari: Ixodidae). J. Med. Entomol. 36: 50-54.
Bruyne M. and Guerin P.M. 1994. Isolation of 2,6 dichlorophenol from the cattle tick Boophilus microplus: receptor cell responses but no evidence for a behavioural response. J. Ins. Physiol. 40: 143-154.
Eiras A.E., Cavalcanti M.G., Mendonça F.A. and Vilela E.F. 1995. Modelo de olfatômetro para avaliar o comportamento de insetos que caminham para a fonte de estímulo. In: XV Congresso Brasileiro de Entomologia 12-17 março, Caxambu, MG., p. 204.
Hamilton J.G.C. and Sonenshine D.E. 1988. Evidence for the occurrence of mounting sex pheromone on the body surface of female Dermacentor variabilis (Say) (Acari: Ixodidae). J. Chem. Ecol. 14: 401-410.
Hamilton J.G.C., Sonenshine D.E. and Lusby W.R. 1989. Cholesteryl oleate: mounting sex pheromone of the hard tick, Dermacentor variabilis (Say) (Acari: Ixodidae). J. Chem. Ecol. 14: 401-410.
Hamilton J.G.C., Papadopoulos E., Harrison S.J., Lloyd C.M. and Walker A.R. 1994. Evidence for mounting sex pheromone in the brown ear tick Rhipicephalus appendiculatus, Neumann 1901 (Acari: Ixodidae). Exp. Appl. Acarol. 18: 331-338.
Khalil G.M., Nada S.A. and Sonenshine D.E. 1981. Sex pheromone regulation of mating behavior in the camel tick, Hyalomma dromedarii (Ixodoidea: Ixodidae). J. Parasitol. 67: 70-76.
Leahy M.G. and Booth K.S. 1983. Attraction of metastriate ticks (Acari: Ixodidae) to the sex pheromone 2,6-dichlorophenol and to substituted phenols. J. Med. Entomol. 20: 104-105.
Malheiro D.M. 1952. Sobre a ocorrência de Otocentor nitens (Neumann, 1897), Cooley, 1938, Acari: Ixodidea-Ixodidae, em cavalos dos estados de São Paulo, Minas Gerais e Mato Grosso. Brasil. Rev. Fac. Med. Vet. S. Paulo 4: 533-535.
Mayer M.S. and McLanghlin J.R. 1991. Hand book of insect pheromones and sex attractants. CRC Press, 1083p.
National Institute of Standards and Technology 1994. PC version of the NIST/EPA/NIH mass spectral data base, version 4.5. U.S. Department of Commerce, Gaithersburg, MD.
Roby T.A. and Anthony D.W. 1963. Transmission of equine piroplasmosis by Dermacentor nitens Neumann. J. Am. Vet. Med. Assoc. 142: 768-769.
Sampaio I.V.B. 1998. Estatística aplicada à experimentação animal. Fundação de Ensino e Pesquisa em Medicina Veterinária, Belo Horizonte, 221 pp.
Sobbhy H., Aggour M.G., Sonenshine D.E. and Burridge M.J. 1994. Cholesteryl esters on the surface of the camel tick, Hyalomma dromedarii (Koch, 1844) and the brown dog tick, Rhipicephalus sanguineus (Latreille, 1806). Exp. Appl. Acarol. 18: 265-280.
Sonenshine D.E. 1985. Pheromones and other semiochemicals of the Acari. Annu. Rev. Entomol. 30: 1-28.
Sonenshine D.E., Silverstein R.M. and West J.R. 1984. Occurrence of sex attractant pheromone, 2,6-dichlorophenol, in relation to age and feeding in American dog tick, Dermacentor vabiabilis (Say) (Acari: Ixodidae). J. Chem. Ecol. 10: 95-100.
Sonenshine D.E., Khalil G.M., Homsher P.J. and Mason S.N. 1982. Dermacentor variabilis and Dermacentor andersoni: genital sex pheromones. Exp. Parasitol. 54: 317-330.
Strickland R.K. and Gerrish R.R. 1964. Distribution of the Tropical Horse Tick in the United States, with notes on associated cases of equine piroplasmosis. J. Am. Vet. Med. Assoc. 144: 875-878.
Wood W.F., Leahy M.G., Galun R., Prestwich G., Meinwald D.J., Parnell R.E. et al. 1975. Phenols as pheromones of ixodid ticks: a general phenomenon? J. Chem. Ecol. 1: 501-506.
Yunker C.E., Keirans J.E., Clifford C.M. and Easton E.R. 1986. Dermacentor ticks (Acari: Ixodidae) of the New World: a scanning electron microscope atlas. Proc. Entomol. Soc. Wash. 88: 609-627.
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Borges, L.M., Eiras, Á.E., Ferri, P.H. et al. The role of 2,6-dichlorophenol as sex pheromone of the tropical horse tick Anocentor nitens (Acari: Ixodidae). Exp Appl Acarol 27, 223–230 (2002). https://doi.org/10.1023/A:1021620215416
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DOI: https://doi.org/10.1023/A:1021620215416