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Molecular analyses of the IGS & ITS regions of rDNA of the psychrophilic yeasts in the genus Mrakia

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Abstract

Species of the genus Mrakia are currently classified as synonyms based on molecular sequence analyses of the large sub-unit ribosomal DNA (LrDNA). Physiological and protein electrophoretic studies, however, reveal possible species differences. To clarify this discrepancy, we undertook molecular sequence analyses of the internal transcribed spacer (ITS) and intergenic spacer (IGS) regions of rDNA from the four psychrophilic Mrakia species and the psychrophilic yeast, Cryptococcus curiosus. Identical ITS sequences were found between C. curiosus, M. nivalis and M. frigida. Although, M. stokesii and M. gelida displayed identical ITS and IGS sequences, their sequences differed from the other three species by 2.3% and 38%, respectively. The results suggest that M. stokesii is a synonym of M. gelida, whereas M. nivalis is a synonym of M. frigida. Sequence differences (1.9%) observed in the IGS region indicates that C. curiosus is a distinct strain of M. frigida.

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References

  • Abyzov SS (1993) Microorganisms in the Antarctic ice. In: Friedman, EI (Ed.). Antarctic Microbiology. (pp 265–295). Wiley-Liss. New York

    Google Scholar 

  • Arthur H & K Watson (1976) Thermal adaptation in yeast: Growth temperatures, membrane lipid, and cytochrome composition of psychrophilic, mesophilic and thermophilic yeasts. J. Bacteriol. 128: 56–58

    Google Scholar 

  • Barnett JA, Payne RW & Yarrow D (1990) Yeasts: Characteristics and Identification. Cambridge Univ Press, Cambridge. pp 291, 435–438

    Google Scholar 

  • Burguess K & Shaw M (1983) Dairy. In: Godfrey T & Reichelt J. (Eds) Industrial Enzymology. The Application of Enzymes in Industry. (pp 260–283). Macmillan. London

    Google Scholar 

  • Deegenaars ML & Watson K (1997) Stress proteins and stress tolerance in an Antarctic, psychrophilic yeast, Candida psychrophila. FEMS Microbiol. Lett. 151: 191–196

    Google Scholar 

  • Deegenaars ML & Watson K (1998) Heat shock response in psychrophilic and psychrotrophic yeast from Antarctica. Extremophiles 2: 241–249

    Google Scholar 

  • di Menna ME (1966) Three new yeasts from Antarctic soils: Candida nivalis, Candida gelida and Candida frigida. Antonie van Leeuwenhoek 32: 25–28

    Google Scholar 

  • Fell JW (1976) Yeasts in Oceanographic regions. In: Jones EBG (Ed.) Recent Advances in Aquatic Mycology. (pp 93–124). Elek Science, London

    Google Scholar 

  • Fell JW & Statzell-Tallman A (1992) Systematic placement of the basidiomycetous yeast Cystofilobasidium lari-marini comb. nov. as predicted by rRNA nucleotide sequence analysis. Antonie van Leeuwenhoek 62: 209–213

    Google Scholar 

  • Fell JW & Statzell-Tallman A (1998) Descriptions of telemorphic basidiomycetous genera and species. In: Kurtzman CP & Fell JW (Eds) The Yeasts, a Taxonomic Study. 4th ed. (pp 676–677). Elsevier Science Publ. Amsterdam

    Google Scholar 

  • Fell JW, Statzell A, Hunter IL & Phaff HJ (1969) Leucosporidium gen. nov. The heterobasidiomycetous stage of several yeasts of the genus Candida. Antonie van Leeuwenhoek 35: 433–462

    Google Scholar 

  • Gounot AM (1991) Bacterial life at low temperature: physiological aspects and biotechnological implications. J. Appl. Bacteriol. 71: 386–397

    Google Scholar 

  • Margesin R & Schinner F (1994) Properties of cold adapted microorganisms and their potential role in biotechnology. J. Biotechnol. 33:1–14

    Google Scholar 

  • Messner R, Prillinger H, Altman F, Lopandic K, Wimmer K, Molnar O & Weigang F (1994) Molecular characterization and application of random amplified polymorphic DNA analysis of Mrakia and Sterigmatomyces species. Int J. Syst. Bacteriol. 44: 694–703

    Google Scholar 

  • Nash CH, Grant DW & Sinclair NA (1968) Thermolability of protein synthesis in cell free systems from the obligately psychrophilic yeast, Candida gelida. Can. J. Microbiol. 15: 339–343

    Google Scholar 

  • Petrescu I, Lamotte-Braaseur J, Chessa JP, Ntarima P, Claeyssens M, Pucci P & Gerday C. (1999) Cold adaptation parameters of the psychrophilic xylanase from the Antarctic yeast strain Cryptococcus adeliensis. Submitted to Extremophiles.

  • Ray MK, Uma Devi K, Seshu Kumar G & Shivaji S (1992) Extracellular protease from the Antarctic yeast Candida humicola. Appl. Environ. Microbiol. 58: 1918–1923

    Google Scholar 

  • Russell NJ (1990) Cold adaptation in microorganisms. Phil. Trans. Roy. Soc. London B. 326: 595–611

    Google Scholar 

  • Russell NJ (1992) Physiology and molecular biology of psychrophilic microorganisms. In: Herbert RA & Sharp RJ (Eds). Molecular Biology and Biotechnology of Extremophiles (pp 203–224). Blackie and Son. London

    Google Scholar 

  • Sharp RJ & Munster MJ (1986) Biotechnological implications for microorganisms from extreme environments. In: Herbert RA & Cod GA (Eds). Microbes in Extreme Environments. (pp 215–295). Academic Press. London

    Google Scholar 

  • Sinclair NA (1978) Role of oxygen in the induction and fermentation in the obligately psychrophilic yeast Leucosporidium stokesii. Can. J. Microbiol. 24: 31–35

    Google Scholar 

  • Travassos, LR & Cury A (1971) Thermophilic enteric yeasts. Annu. Rev. Microbiol. 25: 49–74

    Google Scholar 

  • Vancanneyt M, Van Lerberge E, Berny JF, Hennebert GL & Kersters K (1992) The application of whole-cell protein electrophoresis for the classification and identification of basidiomycetous yeasts. Antonie Van Leeuweenhoek 61: 69–78

    Google Scholar 

  • Vishniac HS (1993) The microbiology of Antarctic soils. In: Friedman EI (Ed.). Antarctic Microbiology (pp. 297–341). Wiley-Liss, New York.

    Google Scholar 

  • Yamada Y & Kawasaki H (1989) The molecular phylogeny of the Q8-equipped basidiomycetous yeast genera Mrakia Yamada et Komagata and Cystofilobasidium Oberwinkler et Bandoni based on the partial sequences of 18S and 26S ribosomal ribonucleic acids. J. Gen. Appl. Microbiol. 35: 173–183

    Google Scholar 

  • Yamada Y & Komagata K (1987) Mrakia gen. nov., a heterobasidiomycetous yeast genus for the Q-8 equipped, self-sporulating organisms which produce a unicellular metabasidium, formerly classified in the genus Leucosporidium. J. Gen. Appl. Microbiol. 33: 455–457

    Google Scholar 

  • Yamada Y & Matsumoto A (1988) An electrophoretic comparison of enzymes in strains of species in the genus Mrakia Yamamada et Komagata (Filobasidiaceae). J. Gen. App. Microbiol. 34: 201–208

    Google Scholar 

  • Yamazaki M, Kurtzman CP & Sugiyama J (1998) Electrophoretic comparison of enzymes. In: Kurtzman CP & Fell JW (Eds) The Yeasts, a taxonomy study. 4th ed. (pp. 49–53). Elsevier Science Publ. Amsterdam.

    Google Scholar 

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Correspondence to Jack W. Fell.

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Diaz, M.R., Fell, J.W. Molecular analyses of the IGS & ITS regions of rDNA of the psychrophilic yeasts in the genus Mrakia. Antonie Van Leeuwenhoek 77, 7–12 (2000). https://doi.org/10.1023/A:1002048008295

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