Chronic exposure to KATP channel openers results in attenuated glucose sensing in hypothalamic GT1-7 neurons
Under a Creative Commons license
open access
Keywords
NN414
Diazoxide
KATP
Glucose sensing
Hypoglycemia
Type 1 diabetes
Abbreviations
CRR
counterregulatory responses
DMEM
Dulbecco's modified Eagle medium
FBS
fetal bovine serum
FCCP
carbonyl cyanide-p-trifluoromethoxyphenylhydrazone
HBS
hepes buffered saline
ICV
intracerebroventricular
KATP
ATP-sensitive potassium channel
KIR6
inward rectifier potassium channel family 6
NN414 (tifenazoxide)
6-chloro-N-(1-methylcyclopropyl)-1,1-dioxo-4H-thieno[3,2-e][1,2,4]thiadiazin-3amine
OCR
oxygen consumption rate
SUR1/2
sulphonylurea receptor 1/2
T1D
Type 1 Diabetes
TTA
Tris base
TES (N-tris(hydroxylmethyl)methyl-1-l-aminoethine sulphonic acid
glacial acetic acid
VMH
ventromedial hypothalamus
2DG
2-deoxy-D-[3H]glucose
ΔVm
membrane potential change
Chemical compounds
Diazoxide (PubChem CID: 3019)
NN414 (Tifenazoxide) (PubChem CID 219048)
Cited by (0)
© 2016 The Authors. Published by Elsevier Ltd.