Journal of Pharmaceutical and Biomedical Analysis
Short communicationSimultaneous determination of six active components in crude and processed Fructus Corni by high performance liquid chromatography
Introduction
Fructus Corni is derived from the dry ripe sarcocarp of Cornus officinalis Sieb. et Zucc. The crude drug or its processed products of jiu zheng pin (JZP) and qing zheng pin (QZP) are used clinically. JZP is produced after steaming the crude drug pre-steeped in wine, and QZP is produced after steaming the crude drug [1], [2].
In the last decades, Fructus Corni has been extensively investigated in phytochemistry, and the results indicated that gallic acid, 5-hydroxymethylfurfural (5-HMF), morroniside, sweroside, loganin and cornuside were the main active components in Fructus Corni. Pharmacological studies on the components showed that they all had good biological activities. Gallic acid had anti-inflammatory activity [3] and bacteriostatic action [4], [5]. Morroniside and loganin showed antioxidation and protective effects on rat mesangial cell proliferation [6]. Sweroside could resist d-aminogalactose hepatic injury [7]. Cornuside suppressed cytokine-induced proinflammatory, relaxated vasculature, and inhibited melanogenesis [8], [9], [10]. 5-HMF could improve blood circulation [11], [12].
To our knowledge, previously reported analytical methods were developed to quantify only one or two types of components in Fructus Corni such as gallic acid [13], [14], [15], morroniside [16] and loganin [17], [18], [19]. In current study, an HPLC-DAD method was developed to quantify six components simultaneously in Fructus Corni.
Section snippets
Samples, chemicals and reagents
Fructus Corni was collected from four suppliers (Henan, Shanxi, Zhejiang and Anhui in China). Gallic acid was purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). 5-HMF was purchased from Shanghai Yousi Biotechnology Co. Ltd. (Shanghai, China). Morroniside, sweroside and loganin were purchased from Institute of Jiangsu Pharmaceutical Research (Nanjing, China). Cornuside was prepared from our laboratory, and its identity was verified
Optimization of chromatographic conditions
Different mobile phase compositions were tested: (1) water–methanol; (2) water–acetonitrile; (3) aqueous phosphoric acid (0.1%, v/v)–acetonitrile phosphoric acid (0.1%, v/v); (4) aqueous ammonium acetate (0.5%, v/v)–acetonitrile. As a result, the combination of aqueous phosphoric acid (0.1%, v/v)–acetonitrile phosphoric acid (0.1%, v/v) for mobile phase was the best for separation. Furthermore, other chromatographic variables were also optimized, including analytical columns (Hanbon Hedera
Conclusion
An HPLC-DAD method has been developed to simultaneously determine six active components in Fructus Corni. This newly established method is validated as simple, precise and accurate. It can be used as a valid analytical method for intrinsic quality control of Fructus Corni.
Acknowledgement
This research was financially supported by the fund of national science and technology research item in “10th Five Year Plan” (2001BA701A11).
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These authors contribute equally to this work.