Elsevier

Environmental Research

Volume 132, July 2014, Pages 54-61
Environmental Research

Impairment of uterine smooth muscle contractions and prostaglandin secretion from cattle myometrium and corpus luteum in vitro is influenced by DDT, DDE and HCH

https://doi.org/10.1016/j.envres.2014.02.018Get rights and content

Highlights

  • DDT, DDE and HCH stimulated bovine myometrial contractions.

  • The studied xenobiotics affect prostaglandin secretion from bovine myometrium and CL.

  • DDT and DDE increased the mRNA expression of LIF, which can stimulate PG production.

  • Prostaglandins are involved in adverse effect of xenobiotics on uterine contractions.

  • DDT, DDE and HCH can reduce reproductive success.

Abstract

The aim of this study was to investigate the effect of dichlorodiphenyltrichloroethane(DDT), dichlorodiphenyldichloroethylene (DDE) and γ-hexachlorocyclohexane (HCH) (10 ng/ml) on myometrial motility and the secretory function of the myometrium and corpus luteum (CL) collected from cows on days 8–12 of the estrous cycle. All of the xenobiotics increased (P<0.05) myometrial contractility. Moreover, the xenobiotics stimulated the secretion of the following prostaglandins (PGs) from myometrial strips: PGF2α, PGE2 and PGI2. DDT and DDE also increased (P<0.05) the release of PGF2α from CL strips, and HCH had the same effect (P<0.05) on the secretion of PGE2 and PGI2. The studied xenobiotics did not affect (P>0.05) PG synthesis, but DDT and DDE increased the mRNA expression levels of leukemia inhibitor factor (LIF), which can stimulate PG production. In summary, the xenobiotics affected PG secretion from cow myometrium and CL, which may contribute to the mechanism of uterine contraction disturbance.

Introduction

1,1,1-Trichloro-2,2,-bis-4-chlorophenyl-ethane (DDT) and γ-hexachlorocyclohexane (HCH), which is commonly referred to as lindane, are recognized as representative organochlorine pesticides from the group of endocrine disruptors. DDT and HCH are currently used in agriculture and forestry in developing countries of Africa and Asia to control pests of crops and to overcome typhoid, malaria (Gunasekaran et al., 2005, Rogan and Chen, 2005, Nweke and Sanders, 2009, Hlongwana et al., 2013) or scabies (Ngueleu et al., 2013). Because of their widespread distribution, lipophilic properties and high resistance to biodegradation (Rogan and Chen, 2005, Tsai, 2010), DDT and HCH are still detected in fodder plants (Pazou et al., 2013) and in the bodies of farm animals (Kamarianos et al., 2003) and humans (Toft et al., 2004, Llop et al., 2010). The production and use of DDT and HCH are banned in the USA, Japan and many European countries (Fromberg et al., 1999, Glynn et al., 2000, Li et al., 2006). However, due to the ability of DDT and HCH to spread by air and water, these pesticides are also a problem in places far from the sources of application (Glynn et al., 2000, Ayotte et al., 2001, Burns et al., 2013). In the environment, DDT is degraded to bis 4-chlorophenyl-1,1-dichloroethene (DDE), which is a stable and ubiquitous component and is also found in living tissues (Turusov et al., 2002, Kamarianos et al., 2003, Meeker et al., 2009). When these pesticides enter organisms, they can act as endocrine disruptors and compete as agonists or antagonists with more than one type of nuclear steroid receptors (Kuiper et al., 1998, Turusov et al., 2002, Li et al., 2008). Therefore, there is increasing concern that pesticides may cause an impairment in the endocrine system, followed by alterations in reproductive physiology.

The unaffected regulation of uterine motility is one of the crucial factors for the initiation and maintenance of pregnancy. Prostaglandins (PGs) are a group of important regulators of the reproductive process, including contractile activity of the myometrium. PGF2α mediates the stimulation of myometrial contractions, but PGE2 and PGI2 can cause the excitation or relaxation of the myometrium (Senior et al., 1993, Coleman et al., 1994). However, the initiation of uterine contractions and/or labor after PGE2 induction may be a result of cervical ripening rather than its direct myometrial effect (Chiossi et al., 2012). The positive feedback loop between luteal oxytocin (OT) and uterine PGF2α in cow is well documented (Skarzynski et al., 1997, Kotwica et al., 1999). However, it has also been reported that LIF, which plays an important role in mammalian implantation, enhances PGE2 production and expression for its uterine receptors (Horita et al., 2007).

Epidemiological observations in humans show a correlation between the presence of DDT, its metabolite (DDE) or HCH in the blood of mothers and an increased risk of miscarriages (Gerhard et al., 1998, Korrick et al., 2001, Longnecker et al., 2005, Venners et al., 2005) and preterm deliveries (Saxena et al., 1980, Longnecker et al., 2001, Torres-Arreola et al., 2003, Pathak et al., 2010, Mustafa et al., 2013). However, data from other studies did not confirm these observations (Berkowitz et al., 1996, Farhang et al., 2005; Fenster et al., 2006; Wood et al., 2007, Cioroiu et al., 2010). In our in vitro study, we showed that DDT and DDE affect the endometrial secretion of PGF2α and PGE2 (M. Wrobel et al., 2009), which are natural regulators of uterine contractions (Senior et al., 1993, Coleman et al., 1994). Because PG secretion could also be followed by the stimulation of myometrial contractions in pregnant cows by these compounds (Mlynarczuk et al., 2010) and because the endometrium is a main source of PGs in the reproductive tract, the involvement of the secreted PGs from the myometrium and corpus luteum (CL) in the adverse effects of organochlorined pesticides on myometrial contractions should be investigated.

Therefore, the aim of this study was to determine the effect of DDT, DDE and HCH on (a) uterine smooth muscle contractions and (b) the synthesis and secretion of PGF2α, PGE2 and PGI2 from myometrial and luteal cells.

Section snippets

Animals and tissues collection

Uterine horns and CL from healthy cows and mature heifers on days 8–12 of the estrous cycle (Ireland et al., 1980, Fields and Fields, 1996) were collected in a commercial slaughterhouse within 20 min after slaughter. These tissues were placed in ice-cold saline and transported to the laboratory within 1 h. Each medium used was supplemented with gentamycin (20 μl/ml). All materials used in these studies were purchased from Sigma-Aldrich (PL) unless otherwise stated.

Uterine strip preparation and incubation

To measure the uterine

Results

Neither myometrial (Fig. 1A) nor luteal (Fig. 1B) cell viability was affected by HCH (P>0.05), in contrast to Act D (P<0.001). Compared to the control, DDT, DDE and HCH increased (P<0.05) the force of contractions with DDE having the greater effect (Fig. 2). All studied xenobiotics increased (P<0.05) the secretion of PGF2α, PGE2 and PGI2 from the myometrium (Fig. 3) compared to the control. Moreover, DDT and DDE increased (P<0.05) PGF2α secretion (Fig. 4A), and HCH increased (P<0.05) the

Discussion

The viability of both myometrial and luteal cells was not affected by HCH exposure. Because DDT and DDE do not affect the lifespan of bovine uterine and ovarian cells at the same used dose (M. Wrobel et al., 2009), we assume that the observed changes in the function of the myometrium and CL were not evoked by cytotoxic effects of these applied compounds.

All tested xenobiotics increased the force of myometrial contractions with DDE exerting the greatest effect. Similarly, DDE, to a greater

Conclusion

Prostaglandins are involved in the mechanism of DDT, DDE and HCH adverse effect on myometrial contractions in cow.

Conflict of interest

The authors declare that there are no conflicts of interest.

Acknowledgments

We would like to thank Professor W.J. Silvia (University of Kentucky, Lexington, KY, USA) and Professor W.W. Thatcher (University of Florida, Gainesville, FL, USA) for the PGFM and PGE2 antisera, respectively. This study was supported by National Science Centre (N N311 082140) and by the Polish Academy of Sciences (ZFiTR/2011).

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