Toxicological and safety evaluation of Nigella sativa lipid and volatile fractions in streptozotocin induced diabetes mellitus

doi:10.1016/S2222-1808(14)60709-X

Asian Pacific Journal of Tropical Disease

Volume 4, Supplement 2, September 2014, Pages S693-S697

https://doi.org/10.1016/S2222-1808(14)60709-X Get rights and content

Abstract

Objective

To evaluate the toxicological aspects of Nigella sativa (N. sativa) lipid and volatile fractions in streptozotocin induced diabetes mellitus.

Methods

National Institute of Health (NIH), Islamabad provided us thirty Sprague Dawley rats that were further divided into three groups, i.e. control, N. sativa lipid fraction (4%) and N. sativa volatile fraction (0.3%), respectively. The serological and haematological indices were evaluated at 4-week intervals during 56 d study.

Results

The results indicated that the diabetes mellitus imparted negative effects on various serological and haematological attributes. However, supplementation of the N. sativa lipid fraction and N. sativa volatile fraction ameliorated the adverse consequences of diabetes mellitus. The diabetes induced renal toxicity and imbalanced serum chemistry were slightly modulated by experimental diets. However, the impact of essential oil was more significant as compared to the fixed oil.

Conclusions

In a nutshell, experimental diets containing N. sativa lipid fraction and N. sativa volatile fraction are effective without having any toxicological effects, and experimental diets reduced toxicological and adverse consequences of diabetes mellitus.

References (25)

PM Guarrera et al.
Perceived health properties of wild and cultivated food plants in local and popular traditions of Italy: a review
J Ethnopharmacol
(2013)
D Patel et al.
Diabetes mellitus: an overview on its pharmacological aspects and reported medicinal plants having antidiabetic activity
Asian Pac J Trop Biomed
(2012)
BK Abel-Salam
Immunomodulatory effects of black seeds and garlic on alloxan-induced diabetes in albino rat
Allergol Immunopathol (Madr)
(2012)
BG Ye et al.
Scientific evaluation of the acute toxicity and 13-week subchronic toxicity of Rheum emodi rhizome extracts in Sprague Dawley rats
Food Chem Toxicol
(2014)
M Tauseef Sultan et al.
Safety assessment of black cumin fixed and essential oils in normal Sprague Dawley, serological and hematological indices
Food Chem Toxicol
(2009)
RC Stanton
Clinical challenges in diagnosis and management of diabetic kidney disease
Am J Kidney Dis
(2014)
L Lu et al.
Safety assessment of the fermented Phylloporia ribis (Lonicera japonica Thunb.) mycelia by oral acute toxicity study in mice and 90-day feeding study in rats
Food Chem Toxicol
(2014)
HY Hung et al.
Recent discovery of plant-derived anti-diabetic natural products
Nat Prod Rep
(2012)
HA Khan et al.
Markers of blood coagulation lipid profile renal function test and serum electrolytes in streptozotocin-induced diabetic rats
Biomed Res
(2012)
S Wild et al.
Global prevalence of diabetes
Diabetes Care
(2004)

MS Butt et al.

Anti-oncogenic perspectives of spices/herbs: a comprehensive review

EXCLI J

(2013)

MS Butt et al.

Selected functional foods for potential in diseases treatment and their regulatory issues

Int J Food Prop

(2013)

Cited by (14)

Anti-hypercholesterolemic and anti-hyperglycaemic effects of conventional and supercritical extracts of black cumin (Nigella sativa)
2017, Asian Pacific Journal of Tropical Biomedicine
Citation Excerpt :
The free radical generation associated with LDL modification can further lead to atherosclerosis [39]. The present findings agree with the findings of Kanter et al. [30]. It was hypothesized the cholesterol deposition was due to increased activities of cholesterol synthesizing enzyme and reduced activities of antioxidants.
To explore the hypoglycaemic and hypocholesterolemic potential of conventional and supercritical extracts of black cumin.
Purposely, rat modelling was carried out for 2 months by designing three studies i.e. study I (normal rats), study II (hyperglycaemic rats) and study III (hypercholesterolemic rats). Each study was further divided into three groups based on diet i.e. control, functional diet (contained extract of black cumin prepared by using conventional solvent) and nutraceutical diet (contained extract of black cumin prepared by supercritical fluid extraction system).
During whole trial, an abating trend was observed in the level of serum cholesterol with maximum reduction (12.8%) in nutraceutical group of study III. Low density lipoprotein and triglyceride level was also lowered maximum in study III as 17.1% and 11.6%, respectively. Whereas, highest decline in glucose level was in nutraceutical group of study II as 11.2%.
Inclusion of black cumin extracts in diet significantly lowers the occurrence of hyperglycaemia and hypercholesterolaemia. Furthermore, hypoglycaemic and hypocholesterolemic potential of nutraceutical diet is more prominent as compared to functional diet.
Citrus peel extract and powder attenuate hypercholesterolemia and hyperglycemia using rodent experimental modeling
2017, Asian Pacific Journal of Tropical Biomedicine
Citation Excerpt :
Resultant peel powder was stored for the extraction of biomolecules and bioevaluation trial. Citrus peel extract was prepared using water and methanol (50% v/v) for time interval of 45 min at 50 °C using the guidelines of Sultan et al. [21], with some modifications trailed by filtration and rotatory evaporation. A rodent trial was conducted to explore the therapeutic potential of the representative citrus peel based functional and nutraceutical diets against lifestyle related metabolic syndrome i.e. hypercholesterolemia and hyperglycemia.
To investigate hypocholesterolemic and hypoglycemic potential of citrus peel extract and powder using rodent experimental modeling.
Considering the fact, rat feeding trial was carried out for a period of 56 d to access the prophylaxis of citrus peel flavonoids by employing normal (study I), hyperglycemic (study II) and hypercholesterolemic (study III) rats. Each study was further divided into three groups to ensure the provision of selected diets, i.e., control, functional and nutraceutical diets. Each study was further divided into three groups to ensure the provision of selected diets, i.e., control, functional and nutraceutical diets.
Declining trend for total cholesterol was observed in all studies with maximum reduction (8.55%) in rat group fed on nutraceutical diet in study III. Likewise, levels of low density lipoproteins and triglycerides reduced 11.39% and 7.89% respectively in hypercholesterolemic rats. Moreover, nutraceutical diet alleviated the sera glucose level by 8.96% in study II.
Conclusively, inclusion of citrus peel bioflavonoids in dietary therapies is a promising strategy to modulate lipidemic and glycemic attributes without imparting any deleterious effect on hematological parameters.
Microencapsulation of Nigella sativa oleoresin by spray drying for food and nutraceutical applications
2016, Food Chemistry
Citation Excerpt :
N. sativa crude oil also contains a small fraction of a volatile oil that has functional properties due to its major biologically active constituent, thymoquinone. Fortunately, this crude oil has low toxicity (Sultan et al., 2014); therefore it can be used safely in nutrition and dietary supplements. Due to these functional properties and advantages, microencapsulation of N. sativa crude oil in spray dried powder is required as an approach to facilitate handling and application in different food and nutraceutical products in addition to potential protection against oxidation.
Oleoresin of Nigella sativa L. (Black cumin) was obtained from the seeds using hexane extraction at room temperature. The oleoresin was emulsified in an aqueous solution containing gum Arabic/maltodextrin (1:1 w/w) and then encapsulated in powder form by spray drying. The characteristics of the obtained powder including moisture content, bulk density, wettability, morphology, encapsulation efficiency were evaluated. The effect of the spray drying on the chemical composition of the volatile oil fraction of N. sativa oleoresin was also evaluated using gas chromatographic–mass spectroscopic analysis. Results indicated that the encapsulation efficiency of the whole oleoresin in the powder can range from 84.2 ± 1.5% to 96.2 ± 0.2% depending on the conditions of extracting the surface oil from the powder. On the other hand the encapsulation efficiency of the volatile oil fraction was 86.2% ±4.7. The formulated N. sativa L. oleoresin powder can be used in the fortification of processed food and nutraceuticals.
Garlic (Allium sativum): Diet based therapy of 21st century-a review
2015, Asian Pacific Journal of Tropical Disease
Functional and nutraceutical foods provide an opportunity to improve one's health by reducing health care costs and to support economic development in rural communities. For this reason, various phyto-based functional foods are becoming popular worldwide owing to number of evidences for their safer therapeutic applications. Garlic (Allium sativum L.,) is an essential vegetable that has been widely utilized as seasoning, flavoring, culinary and in herbal remedies. The consumption of traditional plants especially garlic has progressively increased worldwide because of their great effectiveness, fewer side effects and relatively low cost. Garlic is well known to contain an array of phytochemicals. These bioactive molecules are playing pivotal role in maintaining human health and having potential to reduce various ailments. It has distinct nutritional profile with special reference to its various bioactive components that can be used in different diet based therapies to cure various life-style related disorders. The present review is an attempt to explore the functional/nutraceutical role of garlic against various threats including dyslipidemia and hyperglycemia, cardiovascular disorders, antioxidant capacity and carcinogenic perspectives.
A Narrative Review on Various Oil Extraction Methods, Encapsulation Processes, Fatty Acid Profiles, Oxidative Stability, and Medicinal Properties of Black Seed (Nigella sativa)
2022, Foods
Development of antibacterial nanofibrous wound dressing and conceptual reaction mechanism to deactivate the viral protein by Nigella sativa extract
2022, Advances in Traditional Medicine

View all citing articles on Scopus

Foundation Project: Supported by University of Putra Malaysia for funding Post-Doctoral Fellowship under Knowledge Transfer Program (KTP), Malaysia (UPM/PEND/1.9.1.2).

Available online 11 Aug 2014

View full text

Article preview

Asian Pacific Journal of Tropical Disease

Abstract

Objective

Methods

Results

Conclusions

References (25)

Perceived health properties of wild and cultivated food plants in local and popular traditions of Italy: a review

J Ethnopharmacol

Diabetes mellitus: an overview on its pharmacological aspects and reported medicinal plants having antidiabetic activity

Asian Pac J Trop Biomed

Immunomodulatory effects of black seeds and garlic on alloxan-induced diabetes in albino rat

Allergol Immunopathol (Madr)

Scientific evaluation of the acute toxicity and 13-week subchronic toxicity of Rheum emodi rhizome extracts in Sprague Dawley rats

Food Chem Toxicol

Safety assessment of black cumin fixed and essential oils in normal Sprague Dawley, serological and hematological indices

Food Chem Toxicol

Clinical challenges in diagnosis and management of diabetic kidney disease

Am J Kidney Dis

Safety assessment of the fermented Phylloporia ribis (Lonicera japonica Thunb.) mycelia by oral acute toxicity study in mice and 90-day feeding study in rats

Food Chem Toxicol

Recent discovery of plant-derived anti-diabetic natural products

Nat Prod Rep

Markers of blood coagulation lipid profile renal function test and serum electrolytes in streptozotocin-induced diabetic rats

Biomed Res

Global prevalence of diabetes

Diabetes Care

Anti-oncogenic perspectives of spices/herbs: a comprehensive review

EXCLI J

Selected functional foods for potential in diseases treatment and their regulatory issues

Int J Food Prop

Cited by (14)

Anti-hypercholesterolemic and anti-hyperglycaemic effects of conventional and supercritical extracts of black cumin (Nigella sativa)

Citrus peel extract and powder attenuate hypercholesterolemia and hyperglycemia using rodent experimental modeling

Microencapsulation of Nigella sativa oleoresin by spray drying for food and nutraceutical applications

Garlic (Allium sativum): Diet based therapy of 21st century-a review

A Narrative Review on Various Oil Extraction Methods, Encapsulation Processes, Fatty Acid Profiles, Oxidative Stability, and Medicinal Properties of Black Seed (Nigella sativa)

Development of antibacterial nanofibrous wound dressing and conceptual reaction mechanism to deactivate the viral protein by Nigella sativa extract

Document headingToxicological and safety evaluation of Nigella sativa lipid and volatile fractions in streptozotocin induced diabetes mellitus

Abstract

Objective

Methods

Results

Conclusions

J Ethnopharmacol

Asian Pac J Trop Biomed

Allergol Immunopathol (Madr)

Food Chem Toxicol

Food Chem Toxicol

Am J Kidney Dis

Food Chem Toxicol

Recent discovery of plant-derived anti-diabetic natural products

Nat Prod Rep

Markers of blood coagulation lipid profile renal function test and serum electrolytes in streptozotocin-induced diabetic rats

Biomed Res

Global prevalence of diabetes

Diabetes Care

Anti-oncogenic perspectives of spices/herbs: a comprehensive review

EXCLI J

Selected functional foods for potential in diseases treatment and their regulatory issues

Int J Food Prop

Document heading
Toxicological and safety evaluation of Nigella sativa lipid and volatile fractions in streptozotocin induced diabetes mellitus