ORIGINAL RESEARCHQuality and quantity of smelt (Osmerus eperlanus L.) sperm in relation to time after hormonal stimulation
Section snippets
INTRODUCTION
In the past, European smelt (Osmerus eperlanus L.) was a fish species abundant in Polish lakes and the Baltic Sea coastal zone. However, due to anthropopression associated with eutrophication, the species is now endangered in Poland [23] and it is necessary to protect the naturally occurring populations of smelt1 . Production of
Source of European smelt, acclimation, and hormonal stimulation
Smelt males were obtained in March 2009 from Lake Święcajty (54° 10.8′N; 27° 46.4′E). The captured fish (n=52; weight: 6.9±2.1 g) were transported to a fish farm in Gawrych Ruda (NE Poland) to acclimate at 5°C. After two days, the fish were transported to the experimental tanks (0.35 m3/tank) in the Department of Ichthyology, University of Warmia and Mazury in Olsztyn. The physical and chemical parameters of water were monitored. Males and females were kept separately. After one-week
Effect of hormonal stimulation on smelt body coloration
The hormonally stimulated fish were characterized by darker body coloration compared to control fish, and the coloration persisted until 72 hours after the stimulation (fig. 1A). In contrast to control fish, a high clustering of dark spots was observed in the dorsal part of the peritoneum of the stimulated fish (fig. 1B).
Effects of hormonal stimulation on testis maturation
Before hormonal stimulation, the ampullae of the cranial lobe of the smelt testes were filled with spermatozoa and were separated by a layer of connective tissue with
DISCUSSION
The results of the current paper expand our previous data [13] and provide evidence that in smelt, hormonal stimulation with GnRHa and a dopamine antagonist is the most effective when semen is collected 48 h rather than 24 h after treatment. We found that the highest volume of semen and the best sperm quality, as indicated by CASA parameters, were obtained 48 h after treatment. Histological analysis of the testis demonstrated that high number of spermatozoa was released from the testis after
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Cited by (20)
A novel technique for mass induction of propagation in small fish species: Hormone immersion
2023, Animal Reproduction ScienceSuccessive seminal collections from Leiarius marmoratus during the reproductive period
2021, Animal Reproduction ScienceEffect of different treatment agents and post-treatment latency times on spermiation stimulation of northern pike (Esox lucius) under controlled conditions
2020, TheriogenologyCitation Excerpt :It was confirmed that some CASA parameters increase significantly with time after the application of the treatment agent. For example, in smelt (Osmerus eperlanus), the PRG, VCL, VSL and ALH parameters increased 48 h after the application of Ovaprim compared to 0 h [14]. The authors provide evidence that in smelt, hormonal treatment with complex of sGnRHa and DRA (domperidone) is most effective when sperm is collected 48 h after treatment rather than after 24 h. No further increases were recorded after 48 h.
Sperm quality in fish: Determinants and affecting factors
2019, TheriogenologyReproduction of endangered river lamprey (Lampetra fluviatilis) in controlled conditions
2019, Animal Reproduction ScienceCitation Excerpt :In the case of fish, hormonal treatment is recommended when large quantities of sperm are required, however, the amount of males available for reproduction is limited (Cejko and Krejszeff, 2016). Such solutions are primarily used in the reproduction of high-economic value (Brzuska, 2000; Judycka et al., 2015) or endangered species (Kowalski et al., 2012; Cejko and Kucharczyk, 2015). In the case of river lamprey in the present study, sperm were collected without hormonal administrations and sperm quality, as measured using the CASA system, was optimal for ensuring the success of fertilization.
Induction of spermiation using Ovaprim™ with topical gill method in the silver rasbora (Rasbora argyrotaenia)
2019, TheriogenologyCitation Excerpt :In this study, the increase in the number of milt volume is directly proportional to the increase of sperm concentration (Fig. 1a and b). Increased sperm concentration occurred when Ovaprim™ was applied up to a dose of 0.35 μl/g and tended to decrease at the doses of 0.45 and 0.55 μl/g. Similar results were found in Etroplus suratensis [33] and some other species [14,34,35] that the hormonal treatment using Ovaprim™/sGnRHa increased sperm density coinciding with increases in milt volume. Contrary to these, in another study, sperm concentration of chub (Leuciscus chepalus) was inversely proportional to the volume of sperm produced, due to a slower spermiation rate than its sperm hydration rate [25].