High-performance liquid chromatography and preliminary pharmacokinetics of rufloxacin and its metabolites, N-desmethylrufloxacin and rufloxacinsulfoxide, in urine of Rhesus monkey Macaca mulatta

doi:10.1016/0378-4347(92)80493-A

Journal of Chromatography B: Biomedical Sciences and Applications

Volume 573, Issue 1, 3 January 1992, Pages 168-172

https://doi.org/10.1016/0378-4347(92)80493-A Get rights and content

Abstract

A gradient high-performance liquid chromatographic method for the quantification of rufloxacin and two of its metabolites in urine, N-desmethylrufloxacin and rufloxacinsulfoxide, has been developed and validated. Monkey urine samples were diluted ten times with distilled water and 20 μl were injected onto a Cp Spher 5-ODS column, 5 μm particle size. The mobile phase was a mixture of 4% acetonitrile and 96% buffer at time 0, which changed linearly over 37 min to 26% acetonitrile and 74% buffer. Detection was achieved at 246 nm. The limit of detection of the three compounds was 0.50 μg/ml. An example of a pharmacokinetic study of rufloxacin and its metabolites in monkeys is shown.

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Short communicationHigh-performance liquid chromatography and preliminary pharmacokinetics of rufloxacin and its metabolites, N-desmethylrufloxacin and rufloxacinsulfoxide, in urine of Rhesus monkey Macaca mulatta

Abstract

J. Chromatogr.

J. Med. Chem.

Eur. J. Clin. Microbiol. Infect. Dis.

Short communication
High-performance liquid chromatography and preliminary pharmacokinetics of rufloxacin and its metabolites, N-desmethylrufloxacin and rufloxacinsulfoxide, in urine of Rhesus monkey Macaca mulatta