Cloning, expression and immunological characterization of Ory s 1, the major allergen of rice pollen

doi:10.1016/0378-1119(95)00527-D

Gene

Volume 164, Issue 2, 27 October 1995, Pages 255-259

https://doi.org/10.1016/0378-1119(95)00527-D Get rights and content

Abstract

We have isolated and characterized a cDNA clone, Ory s 1, encoding a group-1 allergen of rice pollen. The Ory s 1 protein shows significant sequence identity to the major allergen of rye-grass pollen, Lol p 1. RNA gel blot analysis shows that the Ory s 1 gene is expressed in mature anthers, but not in vegetative or other floral tissues tested. Southern blot analysis indicates that this clone represents a member of a small gene family in rice. Western blot analyses of total rice pollen proteins with the group-1 allergen-specific monoclonal 3A2 and IgE antibodies from grass pollen-allergic patients, revealed the presence of cross-reactive antigenic and allergenic epitopes in Ory s 1.

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Citation Excerpt :
Related to the β-expansins, these proteins account for most of the major group I allergens of grasses (Sampedro and Cosgrove, 2005), with which they share high sequence similarity (Jiang et al., 2005). In rice, pollen proteins containing this characteristic protein motif include Ory s 1, which has been validated as an allergen on the basis of its recognition by IgE antibodies from allergic individuals (Xu et al., 1995), and Ory s 2, which represents a smaller protein containing a similar motif. Extensive Supplementary data complement this and other topics in the results.
Pollen allergens are among the most abundantly transcribed and translated products in the life history of plants, and particularly grasses. To identify different pollen allergens in rice, putative allergens were identified in the rice genome and their expression characterized using the Affymetrix 57K rice GeneChip microarray. Among the most abundant pollen-specific candidate transcripts were Ory s 1 beta-expansin, Ory s 2, Ory s 7 EF hand, Ory s 11, Ory s 12 profilin A, Ory s 23, glycosyl hydrolase family 28 (polygalacturonase), and FAD binding proteins. Highly expressed pollen proteins are frequently present in multiple copy numbers, sometimes with mirror images located on nearby regions of the opposite DNA strand. Many of these are intronless and inserted as copies that retain nearly exact copies of their regulatory elements. Ory s 23 reflects low variability and high copy number, suggesting recent gene amplification. Some copies contain pseudogenes, which may reflect their origin through activity of retrotransposition; some putative allergenic sequences bear fusion products with repeat sequences of transposable elements (LTRs). The abundance of nearby repetitive sequences, activation of transposable elements, and high production of mRNA transcripts appear to coincide in pollen and may contribute to a syndrome in which highly transcribed proteins may be copied and inserted with streamlined features for translation, including grouping and removal of introns.
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Short communicationCloning, expression and immunological characterization of Ory s 1, the major allergen of rice pollen

Abstract

Anal. Biochem.

Mol. Immunol.

FEBS Lett.

J. Allergy

J. Biol. Chem.

Mol. Immunol.

Human IgE reactivity to purified recombinant and native grass allergens

J. Allergy Clin. Immun.

Cytokinin regulation of a soybean pollen allergen gene

Plant Mol. Biol.

A plant DNA minipreparation:Version II

Plant Mol. Biol. Rep.

Identification and localization of allergenic determinants on grass group 1 antigens using monoclonal antibodies

J. Immunol.