Rapid communicationDetection of Neospora caninum DNA by the polymerase chain reaction
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Cited by (96)
Neospora caninum DNA in feces of crab-eating fox (Cerdocyon thous – Linnaeus, 1776) from northeastern Brazil
2019, Acta TropicaCitation Excerpt :Several studies reported wild canid seropositive for N. caninum in Brazil (Cañón-Franco et al., 2003; Vitaliano et al., 2004; Silva et al., 2005; Mattos et al., 2008; Andre et al., 2010; Almeida et al., 2018). The ITS-1 region of N. caninum is tolerant to mutations (Payne and Ellis, 1996). It can be observed in the phylogenetic tree, which was characterized by presenting a single grouping of N. caninum, without differences among the geographical origin of the sample.
Antibody kinetics in goats and conceptuses naturally infected with Neospora caninum
2013, Veterinary ParasitologyOocysts and high seroprevalence of Neospora caninum in dogs living in remote Aboriginal communities and wild dogs in Australia
2012, Veterinary ParasitologyCitation Excerpt :PCR was performed on all extracted DNA samples using N. caninum-specific primers, Np6plus and Np21plus, that amplify the Nc5 polymorphic region (Müller et al., 1996). Dog faecal DNA samples that exhibited positive bands of the expected size for this marker gene (∼330 bp) were validated by amplifying the internal transcribed spacer 1 of the rDNA (ITS1) using the primer pair JS4 and Tim 11 (Payne and Ellis, 1996; Šlapeta et al., 2002b). Two-graph receiver-operating characteristic (TG-ROC) analysis was used to assess the diagnostic performance of the cELISA results against the IFAT results (Greiner, 1995; Reichel and Pfeiffer, 2002).
Genetic analysis and development of species-specific PCR assays based on ITS-1 region of rRNA in bovine Eimeria parasites
2010, Veterinary ParasitologyCitation Excerpt :Knowledge of apicomplexa at the genomic level has been progressing continuously, and certain molecular methods for the species identification have been presented using PCR technique (Burg et al., 1989; Stucki et al., 1993; Müller et al., 1996; Tsuji et al., 1997). In these methods, one of attractive genomic DNA target is the internal transcribed spacer 1 (ITS-1) region derived from the ribosomal RNA (rRNA) genes (Cai et al., 1992; Payne and Ellis, 1996; Schnitzler et al., 1998, 1999). This region is separated between the 3′ end of 18S rRNA gene and the 5′ end of the 5.8S rRNA gene in each transcription unit.
Australian dingoes are definitive hosts of Neospora caninum
2010, International Journal for ParasitologyCitation Excerpt :Extracted DNA from all samples were resuspended in a final volume of 200 μL (Qiagen QIAamp) or 50 μL (MP – FastDNA Soil) and tested by PCR for the presence of N. caninum DNA using N. caninum-specific primers, Np6plus (5′-CTC GCC AGT CAA CCT ACG TCT TCT-3′) and Np21plus (5′-CCC AGT GCG TCC AAT CCT GTA AC-3′) based on the Nc5 region (Müller et al., 1996). Moreover, internal transcribed spacer 1 of the rDNA (ITS1) was amplified using JS4 (5′-CGA AAT GGG AAG TTT TGT GAA C-3′) and Tim11 (5′-CAC TGA AAC AGA CGT ACC-3′) (Payne and Ellis, 1996; Šlapeta et al., 2002a). Both regions were amplified using 2×MasterMix (Fermentas, USA) or 2×EconoTaq PLUS GREEN MasterMix (Lucigen, USA).