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Genetic Diversity of the Pearse’s Mudskipper Periophthalmus novemradiatus (Perciformes: Gobiidae) and Characterization of its Complete Mitochondrial Genome

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Abstract

Present study provides a first characterization of the complete mitochondrial DNA (mtDNA) genome of the Pearse’s mudskipper Periophthalmus novemradiatus and describes its population genetics based on mtDNA cytochrome c oxidase subunit 1 (co1) gene. The whole mitogenome of P. novemradiatus is the fourth representative for its genus that consists of 23 formally described species to date. Eighty samples from six localities in Setiu Wetlands, Terengganu, Malaysia were sequenced at partial co1 gene region and analyzed, revealing an overall moderate haplotype diversity (h = 0.613) and low nucleotide diversity (π = 0.20%). Moreover, both the neutrality tests and mismatch distribution analyses of co1 suggest that the mudskipper populations might experience a genetic bottleneck or founder event followed by rapid expansion and accumulation of mutations dating approximately 46,251 years ago. Hap01 was the most dominant (61.13%) haplotype that present at all sampling locations, while five haplotypes were respectively observed in two to four locations, and nine haplotypes were singleton. This supports the close relationship of P. novemradiatus in Setiu Wetlands, as also corroborated in the population pairwise FST and genetic distance analyses. Interestingly, genetic propinquity with P. novemradiatus at Java and Bali, Indonesia (the only available sequences in GenBank) was also apparent, suggesting a common source of origin or long range dispersal ability of its planktonic larvae (> 1600 km). The lack of phylogeographic structure suggests there is no evidence of a barrier to gene flow between the South China Sea and Java/ Bali Sea and such pattern is most likely driven by ecological and environmental factor. Preservation of all the P. novemradiatus habitats in Setiu Wetlands is highly recommended because they each possess unique haplotypes present only at the respective location.

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Acknowledgements

We thank Miss Noorhani Syahida binti Kasim for her assistance in preparing the voucher specimen in this work.

Funding

This work was supported by the Niche Research Grant Scheme (NRGS), Universiti Malaysia Terengganu (grant number NRGS/2015/53131/26).

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Authors and Affiliations

  1. School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia

    Min Pau Tan, Mohd Hamzah Nabilsyafiq, Tun Nurul Aimi Mat Jaafar & Muhd Danish-Daniel

  2. Institute of Marine Biotechnology (IMB), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia

    Min Pau Tan, Mohd Nor Siti Azizah, Muhd Danish-Daniel & Yeong Yik Sung

  3. Institut Biodiversiti Tropika dan Pembangunan Lestari (BIO-D Tropika), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia

    Min Pau Tan

  4. Centre for Integrative Ecology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria, 3220, Australia

    Han Ming Gan

  5. Institute of Oceanography and Environment (INOS), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia

    Abd. Ghaffar Mazlan

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  1. Min Pau Tan
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Tan, M.P., Gan, H.M., Nabilsyafiq, M.H. et al. Genetic Diversity of the Pearse’s Mudskipper Periophthalmus novemradiatus (Perciformes: Gobiidae) and Characterization of its Complete Mitochondrial Genome. Thalassas 36, 103–113 (2020). https://doi.org/10.1007/s41208-019-00189-5

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