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Rapid determination of main constituents of packed juices by reverse phase-high performance liquid chromatography: an insight in to commercial fruit drinks

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Abstract

The present work reports the compositional analysis of thirteen different packed fruit juices using high performance liquid chromatography (HPLC). Vitamin C, organic acids (citric and malic) and sugars (fructose, glucose and sucrose) were separated, analyzed and quantified using different reverse phase methods. A new rapid reverse phase HPLC method was developed for routine analysis of vitamin C in fruit juices. The precision results of the methods showed that the relative standard deviations of the repeatability and reproducibility were <0.05 and <0.1 respectively. Correlation coefficient of the calibration models developed was found to be higher than 0.99 in each case. It has been found that the content of Vitamin C was less variable amongst different varieties involved in the study. It is also observed that in comparison to fresh juices, the packed juices contain lesser amounts of vitamin C. Citric acid was found as the major organic acids present in packed juices while maximum portion of sugars was of sucrose. Comparison of the amount of vitamin C, organic acids and sugars in same fruit juice of different commercial brands is also reported.

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Acknowledgement

Authors are thankful to Department of Biotechnology, India for providing the financial support (Grant No. BT/PR7221/MED/14/970/2006).

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Authors and Affiliations

  1. Optical Radiation Standards, National Physical Laboratory, Council of Scientific and Industrial Research, New Delhi, Dr K S Krishnan Marg, New Delhi, 110012, India

    Gunjan Tyagi, Deepak Kumar Jangir, Parul Singh & Ranjana Mehrotra

  2. Department of Physics, Indian Institute of Sciences, Bangalore, 560012, India

    R. Ganesan & E. S. R. Gopal

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  1. Gunjan Tyagi
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  2. Deepak Kumar Jangir
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  3. Parul Singh
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  4. Ranjana Mehrotra
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  5. R. Ganesan
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  6. E. S. R. Gopal
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Correspondence to Ranjana Mehrotra.

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Tyagi, G., Jangir, D.K., Singh, P. et al. Rapid determination of main constituents of packed juices by reverse phase-high performance liquid chromatography: an insight in to commercial fruit drinks. J Food Sci Technol 51, 476–484 (2014). https://doi.org/10.1007/s13197-011-0502-1

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  • DOI: https://doi.org/10.1007/s13197-011-0502-1

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