Abstract
We developed 45 microsatellite markers for three lizard species with fire-related distributions: Amphibolurus norrisi, Ctenotus atlas and Nephrurus stellatus (17, 12 and 16 markers respectively). To isolate microsatellites we used an enrichment technique for N. stellatus and next-generation sequencing for A. norrisi and C. atlas. Fluorescent tags were attached to primers during PCR for flexible genotyping. All loci were polymorphic with 2–24 alleles and expected heterozygosities of 0.043–0.927. These markers will facilitate studies of post-fire dispersal and recolonisation.
References
Bonin A, Bellemain E, Bronken Eidesen P, Pompanon F, Brochmann C, Taberlet P (2004) How to track and assess genotyping errors in population genetics studies. Mol Ecol 13:3261–3273
Clarke MF (2008) Catering for the needs of fauna in fire management: science or just wishful thinking? Wildl. Res 35:385–394
DeWoody J, Nason JD, Hipkins VD (2006) Mitigating scoring errors in microsatellite data from wild populations. Mol Ecol Notes 6:951–957
Driscoll DA, Henderson MK (2008) How many common reptile species are fire specialists? A replicated natural experiment highlights the predictive weakness of a fire succession model. Biol Conserv 141:460–471
Gardner MG, Cooper SJB, Bull CM, Grant WN (1999) Isolation of microsatellite loci from a social lizard, Egernia stokesii, using a modified enrichment procedure. J Hered 90:301–304
Gardner MG, Sanshez JJ, Dudaniec RY, Rheinberger L, Smith AL, Saint KM (2008) Tiliqua rugosa microsatellites: isolation via enrichment and characterisation of loci for multiplex PCR in T. rugosa and the endangered T. adelaidensis. Conserv Genet 9:233–237
Hayden MJ, Nguyen TM, Waterman A, Chalmers KJ (2008) Multiplex-Ready PCR: a new method for multiplexed SSR and SNP genotyping. BMC Genomics 9:80
Hochberg Y (1988) A sharper Bonferroni procedure for multiple tests of significance. Biometrika 75:800–802
Meglécz E (2007) MICROFAMILY (version 1): a computer program for detecting flanking-region similarities among different microsatellite loci. Mol Ecol Notes 7:18–20
Meglécz E, Costedoat C, Debut V, Gilles A, Malausa T, Pech N, Martin J-F (2010) QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinformatics 26(3):403–404
Murphy AR, Gardner MG, Fox SF (2009) Isolation of microsatellites via enrichment and a sequence tagged method in a South American lizard with suspected parental care, Liolaemus leopardinus. Conserv Genet Resour 1(1):13–16. doi:10.1007/s12686-009-9003-1
Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295
Raymond M, Rousset F (1995) GENEPOP (Version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Whelan RJ, Rodgerson L, Dickman CR, Sutherland EF (2002) Critical life cycles of plants and animals: developing a process-based understanding of population changes in fire-prone landscapes. In: Bradstock RA, Williams JE, Gill AM (eds) Flammable Australia: the fire regimes and biodiversity of a continent. Cambridge University Press, Cambridge
Acknowledgments
This research was supported by funds from the Australian Research Council and the Government of South Australia’s Wildlife Conservation Fund. Thanks for technical support go to Kathy Saint, Alison Fitch, Steve Donnellan and Terry Bertozzi at the South Australian Museum.
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Smith, A.L., Gardner, M.G., Bull, C.M. et al. Primers for novel microsatellite markers in “fire-specialist” lizards (Amphibolurus norrisi, Ctenotus atlas and Nephrurus stellatus) and their performance across multiple populations. Conservation Genet Resour 3, 345–350 (2011). https://doi.org/10.1007/s12686-010-9359-2
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DOI: https://doi.org/10.1007/s12686-010-9359-2