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Aerobic rice genotypes displayed greater adaptation to water-limited cultivation and tolerance to polyethyleneglycol-6000 induced stress

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Abstract

Water scarcity and drought have seriously threatened traditional rice cultivation practices in several parts of the world including India. In the present investigation, experiments were conducted to see if the water-efficient aerobic rice genotypes developed at UAS, Bangalore (MAS25, MAS26 and MAS109) and IRRI, Philippines (MASARB25 and MASARB868), are endowed with drought tolerance or not. A set of these aerobic and five lowland high-yielding (HKR47 and PAU201, Taraori Basmati, Pusa1121 and Pusa1460) indica rice genotypes were evaluated for: (i) yield and yield components under submerged and aerobic conditions in field, (ii) root morphology and biomass under aerobic conditions in pots in the nethouse, (iii) PEG-6000 (0, −1, −2 and −3 bar) induced drought stress at vegetative stage using a hydroponic culture system and (iv) polymorphism for three SSR markers associated with drought resistance traits. Under submerged conditions, the yield of aerobic rice genotypes declined by 13.4–20.1 % whereas under aerobic conditions the yield of lowland indica/Basmati rice varieties declined by 23–27 %. Under water-limited conditions in pots, aerobic rice genotypes had 54–73.8 % greater root length and 18–60 % higher fresh root biomass compared to lowland indica rice varieties. Notably, root length of MASARB25 was 35 % shorter than MAS25 whereas fresh and dry root biomass of MASARB25 was 10 % and 64 % greater than MAS25. The lowland indica were more sensitive to PEG-stress with a score of 5.9–7.6 for Basmati and 6.1–6.7 for non-aromatic indica rice varieties, than the aerobic rice genotypes (score 2.7–3.3). A set of three microsatellite DNA markers (RM212, RM302 and RM3825) located on chromosome 1 which has been shown to be associated with drought resistance was investigated in the present study. Two of these markers (RM212 and RM302) amplified a specific allele in all the aerobic rice genotypes which were absent in lowland indica rice genotypes.

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Acknowledgements

This work was partially funded by the Department of Science and Technology, New Delhi. The first author gratefully acknowledges the financial assistance (Junior Research Fellowship) provided by the Department of Science and Technology, Panchkula, Haryana.

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Authors and Affiliations

  1. Department of Molecular Biology and Biotechnology, CCS Haryana Agricultural University, Hisar, 125004, India

    Nitika Sandhu & R. K. Jain

  2. Department of Biochemistry, CCS Haryana Agricultural University, Hisar, 125004, India

    Sunita Jain

  3. Department of Genetics and Plant Breeding, Rice Research Station, Kaul, 136021, India

    K. R. Battan

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  1. Nitika Sandhu
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  2. Sunita Jain
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  3. K. R. Battan
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  4. R. K. Jain
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Correspondence to R. K. Jain.

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Sandhu, N., Jain, S., Battan, K.R. et al. Aerobic rice genotypes displayed greater adaptation to water-limited cultivation and tolerance to polyethyleneglycol-6000 induced stress. Physiol Mol Biol Plants 18, 33–43 (2012). https://doi.org/10.1007/s12298-011-0094-2

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