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Optimisation of Batch Culture Conditions for Cell-Envelope-Associated Proteinase Production from Lactobacillus delbrueckii subsp. lactis ATCC® 7830™

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Abstract

Using a combination of conventional sequential techniques, the batch growth conditions for the production of cell-envelope-associated proteinases have for the first time been studied and optimised in Lactobacillus delbrueckii subsp. lactis 313 (ATCC 7830; LDL 313). Concentrations of inoculum (0.1 < X < 10 % vol/vol), agitation speed (0 < S < 200 rpm), varying incubation temperature (30 < T < 50 °C), starting pH (4.5 < pH < 7) and carbon/nitrogen ratio of production medium (0.2 < r < 5) had an individual effect on proteinase yield (p < 0.01). Optimal conditions for proteinase production included an initial pH of 6.0, 45 °C incubation temperature, 2 % (v/v) inoculum size of OD560 = 1, 150 rpm agitation speed, and growth medium carbon/nitrogen ratio of 1.0. Maximum proteinase activity obtained for whole cells was 0.99 U/ml after 8 h of incubation. The variables studied are very relevant due to their significance in improving the productivity of proteinase synthesis from LDL 313, under process and, likely, economic optimum conditions.

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Acknowledgement

The authors wish to thank the Monash University Graduate School for providing funding for this study.

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  1. Bioengineering Laboratory, Department of Chemical Engineering, Monash University, Wellington Road, Clayton, Victoria, 3800, Australia

    Dominic Agyei, Ravichandra Potumarthi & Michael K. Danquah

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  1. Dominic Agyei
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  2. Ravichandra Potumarthi
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  3. Michael K. Danquah
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Correspondence to Dominic Agyei.

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Agyei, D., Potumarthi, R. & Danquah, M.K. Optimisation of Batch Culture Conditions for Cell-Envelope-Associated Proteinase Production from Lactobacillus delbrueckii subsp. lactis ATCC® 7830™. Appl Biochem Biotechnol 168, 1035–1050 (2012). https://doi.org/10.1007/s12010-012-9839-9

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