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Antioxidant Properties and Rutin Content of High Pressure-Treated Raw and Roasted Buckwheat Groats

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Abstract

The effect of high hydrostatic pressure treatment (200 MPa for 2, 4, and 9 min) on the total antioxidant capacity (TAC), reducing capacity (TRC), and rutin content of raw and roasted buckwheat groats was studied by an updated analytical strategy. TAC and TRC were calculated as the overall capacity of hydrophilic and lipophilic antioxidants. Antioxidant capacity was measured against the 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonate) radical cation (ABTS•+) and the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), and by the photo-induced chemiluminescence assay against the superoxide anion radical (O −•2 ), whereas the reducing capacity was evaluated with the Folin-Ciocalteu reagent (FCR) and directly by the cyclic voltammetry method. Rutin content was determined by HPLC analysis. The TAC of pressure-treated raw and roasted groats was 16–20% and 12.5–17% lower in comparison with untreated groats, respectively. Hydrophilic antioxidants were the main contributor to the TAC of pressure-treated raw and roasted groats. High pressure treatment of raw groats lowered the FCR reducing capacity of hydrophilic antioxidants by an average of 12.6% and 12%, respectively. In contrast, the pressurization of roasted groats increased radical scavenging capacity by 18% on average due to the presence of hydrophilic compounds in roasted groats. The variations in the antioxidant properties of pressure-treated raw groats were accompanied by an average 47% drop in rutin content, whereas irregular changes were noted in HP-treated and roasted material.

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References

  • Barba, F. J., Cortes, C., Esteve, M. J., & Frigola, A. (2011). Study of antioxidant capacity and quality parameters in an orange juice–milk beverage after high-pressure processing treatment. Food and Bioprocess Technology. doi:10.1007/s11947-011-0570-2.

  • Błaszczak, W., Doblado, R., Frias, J., Vidal-Valverde, C., Sadowska, J., & Fornal, J. (2007). Microstructural and biochemical changes in raw and germinated cowpea seeds upon high-pressure treatment. Food Research International, 40, 415–423.

    Article  Google Scholar 

  • Chevion, S., Roberts, M. A., & Chevion, M. (2000). The use of cyclic voltammetry for the evaluation of antioxidant capacity. Free Radical Biology & Medicine, 28, 860–870.

    Article  CAS  Google Scholar 

  • Fabjan, N., Rode, J., Kosir, I. J., Wang, Z. H., Zhang, Z., & Kreft, A. I. (2003). Tartary buckwheat (Fagopyrum tataricum Gaertn.) as a source of dietary rutin and quercitin. Journal of Agricultural and Food Chemistry, 51, 6452–6455.

    Article  CAS  Google Scholar 

  • General Convention and Statistics. (1995). In: Statistica for Windows, vol. I-III, 2nd Edition, Ed, by Statsoft Inc., Tulsa, USA.

  • Holasova, M., Fiedlerowa, V., Smrcinova, H., Orsak, M., Lachman, J., & Vavreinova, S. (2002). Buckwheat—the source of antioxidant activity in functional foods. Food Research International, 35, 207–211.

    Article  CAS  Google Scholar 

  • Huang, D., Ou, B., & Prior, R. L. (2006). The chemistry behind antioxidant capacity assays. Journal of Agricultural and Food Chemistry, 53, 1841–1856.

    Article  Google Scholar 

  • Katalinic, V., Milos, M., Kulisic, T., & Juki, M. (2006). Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chemistry, 94, 550–557.

    Article  CAS  Google Scholar 

  • Kilmartin, P. A., & Hsu, C. F. (2003). Characterisation of polyphenols in green, oolong, and black teas, and in coffee, using cyclic voltammetry. Food Chemistry, 82, 501–512.

    Article  CAS  Google Scholar 

  • Kilmartin, P. A., Zou, H., & Waterhouse, A. L. (2001). A cyclic voltammetry method suitable for characterizing antioxidant properties of wine and wine phenolics. Journal of Agricultural and Food Chemistry, 49, 1957–1965.

    Article  CAS  Google Scholar 

  • Kreft, I., Fabjan, N., & Yasumoto, K. (2006). Rutin content in buckwheat (Fagopyrum esculentum Moench) food materials and products. Food Chemistry, 98, 508–512.

    Article  CAS  Google Scholar 

  • Magalhaes, L. M., Segundo, M. A., Reis, S., & Lima, J. L. F. C. (2008). Methodological aspects about in vitro evaluation of antioxidant properties. Analytica Chimica Acta, 613, 1–19.

    Article  CAS  Google Scholar 

  • Matser, A. M., Knott, E. R., Teunissen, P. G. M., & Bartels, P. V. (2000). Effect of hydrostatic pressure on mushrooms. Journal of Food Engineering, 45, 11–16.

    Article  Google Scholar 

  • Matser, A. M., Krebbers, B., van den Berg, R., & Bartels, P. V. (2004). Advantages of high pressure sterilization on quality of food products. Trends in Food Science and Technology, 15, 79–85.

    Article  CAS  Google Scholar 

  • McInerney, J. K., Seccafien, C. A., Stewart, C. M., & Bird, A. R. (2007). Effect of high pressure processing on antioxidant activity, and total carotenoid content and availability, in vegetables. Innovative Food Sciences & Emerging Technologies, 8, 543–548.

    Article  CAS  Google Scholar 

  • Norton, T., & Sun, D.-W. (2008). Recent advances in the use of high pressure as an effective processing technique in the food industry. Food and Bioprocess Technology, 1, 2–34.

    Article  Google Scholar 

  • Popov, I., & Lewin, G. (1999). In: Packer L (ed) Oxidants and antioxidants. Academic Press, New York and London, part B, pp 96–100

  • Prior, R. L., Wu, X., & Schaich, K. (2005). Standardized methods for the determination of antioxidant capacity and phenolics in foods, and dietary supplements. Journal of Agricultural and Food Chemistry, 53, 4290–4302.

    Article  CAS  Google Scholar 

  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology & Medicine, 26, 1291–1237.

    Article  Google Scholar 

  • Roldan-Martin, E., Sanchez-Moreno, C., Lioria, R., de Ancos, B., & Cano, M. P. (2009). Onion high-pressure processing: flavonol content and antioxidant activity. LWT-Food Sciences and Technology, 42, 835–841.

    Article  Google Scholar 

  • San Martin, M. F., Barbosa-Canovas, G. V., & Swanson, B. G. (2002). Food processing by high hydrostatic pressure. Critical Reviews in Food Science and Nutrition, 42, 627–645.

    Article  CAS  Google Scholar 

  • Sensoy, I., Rosen, R. T., Ho, Ch-T, & Karwe, M. V. (2006). Effect of processing on buckwheat phenolics and antioxidant activity. Food Chemistry, 99, 388–393.

    Article  CAS  Google Scholar 

  • Shahidi, F., & Naczk, M. (1995). In F. Shahidi & M. Naczk (Eds.), Food phenolic: sources, chemistry, effects and applications (pp. 287–293). Lancaster, PA: Technomic Publishing Company.

    Google Scholar 

  • Singleton, V. L., Orthofer, R., & Lamuela-Raventos, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152–178.

    Article  CAS  Google Scholar 

  • Trejo Araya, X. I. T., Hendrickx, M., Verliden, B. E., Van Buggenhout, S., Smale, N. J., Stewart, C., et al. (2007). Understanding texture changes of high pressure processed fresh carrots: a microstructural and biochemical approach. Journal of Food Engineering, 80, 873–884.

    Article  Google Scholar 

  • Zhang, M., Chen, H., Li, J., Pei, Y., & Liang, Y. (2010). Antioxidant properties of tartary buckwheat extracts as affected by different thermal processing methods. LWT- Food Science and Technology, 43, 181–185.

    Article  CAS  Google Scholar 

  • Zielińska, D., & Zieliński, H. (2011). Antioxidant activity of flavone C-glucosidesdetermined by updated analytical strategies. Food Chemistry, 124, 672–678.

    Article  Google Scholar 

  • Zielinska, D., Szawara-Nowak, D., Ornatowska, A., & Wiczkowski, W. (2007). The use of cyclic voltammetry, photochemiluminescence and spectrophotometric methods for the measurement of the antioxidant capacity of buckwheat sprouts. Journal of Agricultural and Food Chemistry, 55, 9891–9898.

    Article  CAS  Google Scholar 

  • Zielińska, D., Szawara-Nowak, D., & Zieliński, H. (2007). Comparison of spectrophotometric and electrochemical methods for the evaluation of antioxidant capacity of buckwheat products after hydrothermal treatment. Journal of Agricultural and Food Chemistry, 55(15), 6124–6131.

    Article  Google Scholar 

  • Zielińska, D., Szawara-Nowak, D., & Zieliński, H. (2009). Antioxidative and anti-glycation activity of bitter buckwheat tea. The European Journal of Plant Science and Biotechnology, 3(SI 1), 79–83.

    Google Scholar 

  • Zielińska, D., Szawara-Nowak, D., & Zieliński, H. (2010). Determination of the antioxidant activity of rutin and its contribution to the antioxidant capacity of diversified buckwheat origin material by updated analytical strategies. Polish Journal of Food and Nutrition Sciences, 60, 315–321.

    Google Scholar 

  • Zieliński, H., Michalska, A., Amigo-Benavent, M., del Castillo, M. D., & Piskula, M. K. (2009). Changes in protein quality and antioxidant properties of buckwheat seeds and groats induced by roasting. Journal of Agricultural and Food Chemistry, 57, 4771–4776.

    Article  Google Scholar 

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Acknowledgments

The study was supported by Project REFRESH (FP7-REGPOT-2010-1-264105)—Unlocking the potential of the Institute of Animal Reproduction and Food Research for strengthening integration with the European Research Area and region development. This project was financed in the area of “Research Potential” of the 7th Framework Programme.

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Correspondence to Wioletta Błaszczak.

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Błaszczak, W., Zielińska, D., Zieliński, H. et al. Antioxidant Properties and Rutin Content of High Pressure-Treated Raw and Roasted Buckwheat Groats. Food Bioprocess Technol 6, 92–100 (2013). https://doi.org/10.1007/s11947-011-0669-5

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