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Engineering of mesoporous silica nanoparticles for release of ginsenoside CK and Rh2 to enhance their anticancer and anti-inflammatory efficacy: in vitro studies

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Abstract

The current study highlights the fabrication of drug delivery system by utilizing 200 nm mesoporous silica nanoparticles (MSNPs) with 4-nm pore size, as a carrier system for delivery ginsenoside compound K (CK) and Rh2 to enhance their efficacy. The two pharmacologically imperative ginsenosides, CK and Rh2, were loaded to the MSNPs to prepare MSNPs-CK and MSNPs-Rh2, respectively. A fluorescein isothiocyanate (FITC) fluorescent dye was combined in the MSNPs carrier system, in order to trace the cellular uptake of ginsenoside-loaded nanoparticles for in vitro studies. Following purification, the so-prepared MSNPs-CK-FITC and MSNPs-Rh2-FITC were characterized by several analytical techniques, which includes, high-pressure liquid chromatography (HPLC), 1H NMR, field emission transmission electron microscopy (FE-TEM), Fourier transform infrared spectroscopy (FT-IR), x-ray diffraction (XRD), thermogravimetric analysis (TGA), and dynamic light scattering (DLS). In vitro cytotoxicity assay in HaCaT skin cells, A549 lung cancer cells, HepG2 liver carcinoma cells, and HT-29 colon cancer cell lines were tested for MSNPs-CK-FITC and MSNPs-Rh2-FITC. The results demonstrate the excellent biocompatibility of nanoparticles in normal cell lines (HaCaT skin cells) and anticancer efficacy in all the tested cancer cell lines at 10-μM concentration. Additionally, the in vitro anti-inflammatory behavior of MSNPs-CK-FITC and MSNPs-Rh2-FITC were checked in RAW264.7 (murine macrophage) cell lines. The outcomes showed higher anti-inflammatory efficacy of MSNPs-CK-FITC and MSNPs-Rh2-FITC as compared to standard ginsenosides CK and Rh2 in RAW264.7 cell lines. Thus, with 200 nm MSNPs carrier system for the delivery ginsenosides CK and Rh2, a high amount of loading and increasing in vitro pharmacological efficacies of ginsenosides were realized. This study may provide useful insights for designing and improving the applicability of MSNPs for ginsenoside delivery.

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References

  • Ahn S, Singh P, Castro-Aceituno V, Yesmin Simu S, Kim YJ, Mathiyalagan R, et al. (2016a) Gold nanoparticles synthesized using Panax ginseng leaves suppress inflammatory—mediators production via blockade of NF-kappaB activation in macrophages . Artif cells Nanomed Biotechnol 1-7

  • Ahn S, Siddiqi MH, Aceituno VC, Simu SY, Zhang J, Perez ZE et al (2016b) Ginsenoside Rg5:Rk1 attenuates TNF-alpha/IFN-gamma-induced production of thymus- and activation-regulated chemokine (TARC/CCL17) and LPS-induced NO production via downregulation of NF-kappaB/p38 MAPK/STAT1 signaling in human keratinocytes and macrophages. In vitro Cell Dev Biol anim 52:287–295

    Article  Google Scholar 

  • Baldwin AS Jr (1996) The NF-kappa B and I kappa B proteins: new discoveries and insights. Annu Rev Immunol 14:649–683

    Article  Google Scholar 

  • Bharti C, Nagaich U, Pal AK, Gulati N (2015) Mesoporous silica nanoparticles in target drug delivery system: a review. Int J Pharm Investig 5:124–133

    Article  Google Scholar 

  • Chen F, Hong H, Shi S, Goel S, Valdovinos HF, Hernandez R et al (2014a) Engineering of hollow mesoporous silica nanoparticles for remarkably enhanced tumor active targeting efficacy. Sci Rep 4:5080

    Article  Google Scholar 

  • Chen J, Wu H, Wang Q, Chang Y, Liu K, Song S et al (2014b) Ginsenoside metabolite compound k alleviates adjuvant-induced arthritis by suppressing T cell activation. Inflammation 37:1608–1615

    Article  Google Scholar 

  • Cho YS, Kim CH, Ha TS, Lee SJ, Ahn HY (2013) Ginsenoside rg2 inhibits lipopolysaccharide-induced adhesion molecule expression in human umbilical vein endothelial cell. Korean J Physiol Pharmacol 17:133–137

  • Fan J, Fang G, Wang X, Zeng F, Xiang Y, Wu S (2011) Targeted anticancer prodrug with mesoporous silica nanoparticles as vehicles. Nanotechnology 22:455102

    Article  Google Scholar 

  • He H, Xiao H, Kuang H, Xie Z, Chen X, Jing X et al (2014) Synthesis of mesoporous silica nanoparticle-oxaliplatin conjugates for improved anticancer drug delivery. Colloids Surf B Biointerfaces 117:75–81

    Article  Google Scholar 

  • Heidegger S, Gossl D, Schmidt A, Niedermayer S, Argyo C, Endres S et al (2016) Immune response to functionalized mesoporous silica nanoparticles for targeted drug delivery. Nano 8:938–948

    Google Scholar 

  • Koninti RK, Palvai S, Satpathi S, Basu S, Hazra P (2016) Loading of an anti-cancer drug into mesoporous silica nano-channels and its subsequent release to DNA. Nano 8:18436–18445

    Google Scholar 

  • Lee CH, Kim JH (2014) A review on the medicinal potentials of ginseng and ginsenosides on cardiovascular diseases. J Ginseng Res 38:161–166

    Article  Google Scholar 

  • Lodha A, Lodha M, Patel A, Chaudhuri J, Dalal J, Edwards M et al (2012) Synthesis of mesoporous silica nanoparticles and drug loading of poorly water soluble drug cyclosporin a. J Pharm Bioallied Sci 4:S92–S94

    Article  Google Scholar 

  • Lu J, Liong M, Sherman S, Xia T, Kovochich M, Nel AE et al (2007) Mesoporous silica nanoparticles for cancer therapy: energy-dependent cellular uptake and delivery of paclitaxel to cancer cells. NanoBiotechnology 3:89–95

    Article  Google Scholar 

  • Mathiyalagan R, Subramaniyam S, Kim YJ, Kim YC, Yang DC (2014) Ginsenoside compound K-bearing glycol chitosan conjugates: synthesis, physicochemical characterization, and in vitro biological studies. Carbohydr Polym 112:359–366

    Article  Google Scholar 

  • Miao W, Zhang C, Cai Y, Zhang Y, Lu H (2016) Fast solid-phase extraction of N-linked glycopeptides by amine-functionalized mesoporous silica nanoparticles. Analyst 141:2435–2440

    Article  Google Scholar 

  • Pinna A, Malfatti L, Galleri G, Manetti R, Cossu S, Rocchitta G, Migheli R, Serra PA, Innocenzi P (2015) Ceria nanoparticles for the treatment of Parkinson like diseases induced by chronic manganese intoxication. RSC Adv 5:20432

    Article  Google Scholar 

  • RE Yanes, F Tamanoi (2012) Development of mesoporous silica nanomaterials as a vehicle for anticancer drug delivery. Ther Deliv. 3(3):389-404

  • Singh N, Karambelkar A, Gu L, Lin K, Miller JS, Chen CS et al (2011) Bioresponsive mesoporous silica nanoparticles for triggered drug release. J Am Chem Soc 133:19582–19585

    Article  Google Scholar 

  • Singh P, Kim YJ, Singh H, Mathiyalagan R, Wang C, Yang DC (2015a) Biosynthesis of anisotropic silver nanoparticles by Bhargavaea indica and their synergistic effect with antibiotics against pathogenic microorganisms. J Nanomater 2015:10

    Google Scholar 

  • Singh P, Kim YJ, Singh H, Wang C, Hwang KH, Farh Mel A et al (2015b) Biosynthesis, characterization, and antimicrobial applications of silver nanoparticles. Int J Nanomedicine 10:2567–2577

    Google Scholar 

  • Singh P, Kim YJ, Yang DC (2015c) A strategic approach for rapid synthesis of gold and silver nanoparticles by Panax ginseng leaves . Artif cells Nanomed Biotechnol 1-9

  • Singh P, Kim YJ, Wang C, Mathiyalagan R, Yang DC (2016a) The development of a green approach for the biosynthesis of silver and gold nanoparticles by using Panax ginseng root extract, and their biological applications Artif cells Nanomed Biotechnol 44:1150-7

  • Singh P, Kim YJ, Wang C, Mathiyalagan R, Yang DC (2016b) Microbial synthesis of flower-shaped gold nanoparticles. Artif Cells Nanomed Biotechnol 44:1469–1474

    Google Scholar 

  • Singh P, Kim YJ, Yang DC (2016c) A strategic approach for rapid synthesis of gold and silver nanoparticles by Panax ginseng leaves. Artif Cells Nanomed Biotechnol 44:1949–1957

    Article  Google Scholar 

  • Singh P, Kim YJ, Zhang D, Yang DC (2016d) Biological synthesis of nanoparticles from plants and microorganisms. Trends Biotechnol 34:588–599

    Article  Google Scholar 

  • Singh P, Singh H, Kim YJ, Mathiyalagan R, Wang C, Yang DC (2016e) Extracellular synthesis of silver and gold nanoparticles by Sporosarcina koreensis DC4 and their biological applications. Enzym Microb Technol 86:75–83

    Article  Google Scholar 

  • Singh P, Singh H, Castro-Aceituno V, Ahn S, Kim YJ, Yang DC (2017a) Bovine serum albumin as a nanocarrier for the efficient delivery of ginsenoside compound K: preparation, physicochemical characterizations and in vitro biological studies. RSC Adv 7:15397

    Article  Google Scholar 

  • Singh P, Kim YJ, Singh H, Ahn S, Castro-Aceituno V, Yang DC (2017b) In situ preparation of water-soluble ginsenoside Rh2-entrapped bovine serum albumin nanoparticles: in vitro cytocompatibility studies. Int J Nanomedicine 12:4073–4084

    Article  Google Scholar 

  • Tang F, Li L, Chen D (2012) Mesoporous silica nanoparticles: synthesis, biocompatibility and drug delivery. Adv Mater 24:1504–1534

    Article  Google Scholar 

  • Tang XP, Tang GD, Fang CY, Liang ZH, Zhang LY (2013) Effects of ginsenoside Rh2 on growth and migration of pancreatic cancer cells. World J Gastroenterol 19(10):1582–1592

    Article  Google Scholar 

  • Wang CZ, Yuan CS (2008) Potential role of ginseng in the treatment of colorectal cancer. Am J Chin Med 36:1019–1028

    Article  Google Scholar 

  • Wang Y, Huang HY, Yang L, Zhang Z, Ji H (2016) Cetuximab-modified mesoporous silica nano-medicine specifically targets EGFR-mutant lung cancer and overcomes drug resistance. Sci Rep 6:25468

    Article  Google Scholar 

  • Wu H, Chen J, Wang Q, Jia X, Song S, Yuan P et al (2014) Ginsenoside metabolite compound K attenuates inflammatory responses of adjuvant-induced arthritis rats. Immunopharmacol Immunotoxicol 36:124–129

    Article  Google Scholar 

  • Yanes RE, Tamanoi F (2014) Development of mesoporous silica nanomaterials as a vehicle for anticancer drug delivery. Ther Deliv 3:389–404

    Article  Google Scholar 

  • Yu T, Hubbard D, Ray A, Ghandehari H (2012) In vivo biodistribution and pharmacokinetics of silica nanoparticles as a function of geometry, porosity and surface characteristics. J Control Release 163:46–54

    Article  Google Scholar 

  • Zhang N, Ding E, Feng X, Xu Y, Cai H (2012) Synthesis, characterizations of dye-doped silica nanoparticles and their application in labeling cells. Colloids Surf B Biointerfaces 89:133–138

    Article  Google Scholar 

Download references

Acknowledgements

This research was supported by a grant from the Next-Generation BioGreen 21 Program (SSAC, grant #PJ0120312016), Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Department of Oriental Medicine Biotechnology and Ginseng Bank, College of Life Sciences, Kyung Hee University, Yongin, 446-701, Republic of Korea

    Priyanka Singh, Hina Singh, Verónica Castro-Aceituno, Sungeun Ahn, Yeon Ju Kim & Deok Chun Yang

  2. Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, Republic of Korea

    Priyanka Singh, Mohamed El-Agamy Farh & Deok Chun Yang

Authors
  1. Priyanka Singh
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  2. Hina Singh
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  3. Verónica Castro-Aceituno
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  4. Sungeun Ahn
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  5. Yeon Ju Kim
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  6. Mohamed El-Agamy Farh
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  7. Deok Chun Yang
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Correspondence to Priyanka Singh, Yeon Ju Kim or Deok Chun Yang.

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Funding

This study was funded by a grant from the Next-Generation BioGreen 21 Program (SSAC, grant #PJ0120312016), Rural Development Administration, Republic of Korea.

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The authors declare that they have no conflict of interest.

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Singh, P., Singh, H., Castro-Aceituno, V. et al. Engineering of mesoporous silica nanoparticles for release of ginsenoside CK and Rh2 to enhance their anticancer and anti-inflammatory efficacy: in vitro studies. J Nanopart Res 19, 257 (2017). https://doi.org/10.1007/s11051-017-3949-9

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