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A set of near-isogenic lines of Indica-type rice variety CO 39 as differential varieties for blast resistance

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Abstract

Twenty-seven near-isogenic lines (NILs) with the genetic background of a blast-susceptible variety, CO 39, were developed by repeated backcrossing as a first set of a large number of differential varieties (DVs) with Indica-type genetic background. The NILs included 14 resistance genes—Pish, Pib, Piz-5, Piz-t, Pi5(t), Pik-s, Pik, Pik-h, Pik-m, Pik-p, Pi1, Pi7(t), Pita, and Pita-2—derived from 26 donor varieties. The reaction patterns of NILs against 20 standard isolates from the Philippines were similar to those of blast monogenic lines with the same resistance gene, except for those against two isolates that are avirulent to Pia in the genetic background of CO 39. A genome-wide DNA marker survey revealed that chromosome segments were introgressed in the regions where each resistance gene was previously mapped and most of the other chromosome regions in each NIL were CO 39 type. Segregation analysis of resistance and co-segregation analysis between resistance and DNA markers using F3 populations derived from the crosses between each NIL and the recurrent parent, CO 39, revealed a single-gene control of resistance and association between resistance and target introgressed segments. The morphological characters of each NIL were almost the same as those of the recurrent parent except for some lines, suggesting that these NILs can be used even under tropical conditions where Japonica-type DVs are not suitable for cropping. Thus, these NILs are useful not only as genetic tools for blast resistance study but also as sources of genes for breeding of Indica-type rice varieties.

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Acknowledgments

We would like to acknowledge the technical assistance of the support staff of the IRRI-Japan Collaborative Project for their dedication and support in this study. This study was conducted under the “Blast Research Network for Stable Rice Production” in JIRCAS funded by the Ministry of Agriculture, Forestry and Fisheries of Japan and the IRRI-Japan Collaborative Research Project Phases III to V, awarded to IRRI by the Ministry of Foreign Affairs and Ministry of Agriculture, Forestry and Fisheries of Japan. We thank the Japan Society for the Promotion of Science for its support of the first author’s PhD dissertation. We also sincerely thank Prof. Atsushi Yoshimura and Associate Prof. Hideshi Yasui of the Plant Breeding Laboratory, Faculty of Agriculture, Kyushu University, Fukuoka, Japan, for helpful suggestions and critical reading and review of the manuscript.

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Authors and Affiliations

  1. International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines

    Mary Jeanie Telebanco-Yanoria, Yohei Koide, Leodegario A. Ebron & Nobuya Kobayashi

  2. Japan International Research Center for Agricultural Sciences, 1-1 Ohwashi, Tsukuba, Ibaraki, 305-8686, Japan

    Yohei Koide, Yoshimichi Fukuta & Nobuya Kobayashi

  3. National Agricultural Research Center for Kyushu Okinawa Region, 2421 Suya, Koshi, Kumamoto, 861-1192, Japan

    Tokio Imbe

  4. National Institute of Crop Science, National Agriculture and Food Research Organization, 3-1-1 Kannondai, Tsukuba, Ibaraki, 305-8517, Japan

    Hiroshi Tsunematsu & Hiroshi Kato

  5. Agricultural Genetics Institute, Conhue, Tuliem, Hanoi, Vietnam

    Thi Minh Nguyet Nguyen

Authors
  1. Mary Jeanie Telebanco-Yanoria
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  2. Yohei Koide
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  3. Yoshimichi Fukuta
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  4. Tokio Imbe
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  5. Hiroshi Tsunematsu
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  6. Hiroshi Kato
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  7. Leodegario A. Ebron
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  8. Thi Minh Nguyet Nguyen
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  9. Nobuya Kobayashi
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Correspondence to Nobuya Kobayashi.

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Mary Jeanie Telebanco-Yanoria and Yohei Koide have contributed equally.

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Telebanco-Yanoria, M.J., Koide, Y., Fukuta, Y. et al. A set of near-isogenic lines of Indica-type rice variety CO 39 as differential varieties for blast resistance. Mol Breeding 27, 357–373 (2011). https://doi.org/10.1007/s11032-010-9437-x

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  • DOI: https://doi.org/10.1007/s11032-010-9437-x

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