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Morpho-agronomic and simple sequence repeat-based diversity in colored rice (Oryza sativa L.) germplasm from peninsular India

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Abstract

Knowledge of the genetic diversity in the germplasm accessions is important for the efficient germplasm management. We studied 45 colored rice accessions, which had been earlier collected from peninsular India. The accessions were evaluated at two diverse locations for 12 morpho-agronomic traits and genotyped using 50 simple sequence repeat (SSR) markers. Significant genotypic variability was observed for all morpho-agronomic traits studied. Dendrogram and principal component analysis based on morpho-agronomic traits separated the accessions into three clusters. The first two principal components accounted for 82% of the total variation. The most discriminatory traits were number of grains per panicle, biomass yield, and days to flowering and maturity. The SSR analysis revealed high polymorphic information content value of 0.84. Though, Mantel test did not show a significant correlation, yet the classification based on phenotyping and genotyping data showed good agreement for the expression of morpho-agronomic traits in the formation of clusters. Five accessions had high concentration (> 100 μg/g) of Fe and one of Zn. Our study revealed the presence of large genetic variation among the colored rice accessions evaluated and moderate agreement between morpho-agronomic and SSR–based classifications and of these with geographic diversity.

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Acknowledgments

SJM thanks Indian Council of Agricultural Research for the award of Junior Research Fellowship. The authors acknowledge the provision of facilities by IARI for field experimentation at New Delhi, and the help received from Dr Mrs. Sachdeva, Nuclear Research Laboratory, IARI, for chemical analyses.

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Authors and Affiliations

  1. National Bureau of Plant Genetic Resources, New Delhi, India

    S. J. M. Gowda, G. J. Randhawa, I. S. Bisht, P. K. Firke & B. S. Dhillon

  2. Indian Agricultural Research Institute, New Delhi, India

    A. K. Singh

  3. National Bureau of Plant Genetic Resources Regional Station, Thrissur, India

    Z. Abraham

  4. Institute of Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, 70593, Stuttgart, Germany

    S. J. M. Gowda & B. S. Dhillon

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  1. S. J. M. Gowda
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  2. G. J. Randhawa
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  3. I. S. Bisht
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  4. P. K. Firke
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  5. A. K. Singh
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  7. B. S. Dhillon
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Correspondence to B. S. Dhillon.

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Gowda, S.J.M., Randhawa, G.J., Bisht, I.S. et al. Morpho-agronomic and simple sequence repeat-based diversity in colored rice (Oryza sativa L.) germplasm from peninsular India. Genet Resour Crop Evol 59, 179–189 (2012). https://doi.org/10.1007/s10722-011-9674-9

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  • DOI: https://doi.org/10.1007/s10722-011-9674-9

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