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Comparative analysis of diversity based on morpho-agronomic traits and microsatellite markers in common bean

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Abstract

Morpho-agronomic traits and microsatellite markers were used to survey genetic diversity in 115 common bean genotypes that included 70 Indian landraces, 24 released varieties and 21 exotic accessions. Twelve morpho-agronomic traits, namely, days to 50% flowering, leaflet length, leaflet width, pod length, pod width, number of pods per plant, days to maturity, seed length, seed width, number of seeds per pod, 100 seed weight and seed yield per plant were studied. Field data of two consecutive years were subjected to multivariate analysis as proposed by Mahalanobis’s D2-statistics, Tochers method of clustering and combined analysis of variance. Seventeen microsatellite markers were also used to examine genetic diversity at molecular level that showed polymorphic information content (PIC) in the range of 0.00–0.684. Dendrograms based on Euclidean distances and UPGMA analysis showed the presence of majority of released varieties into single cluster, which pointed toward their low genetic base in comparison to indigenous landraces and exotic germplasm. Significant correlation existed between morphological genetic distance and microsatellite genetic distance tested by Mantel test (r = 0.876).

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Acknowledgments

Financial support received from DBT (Department of Biotechnology, Government of India, New Delhi) for carrying out this research is greatly acknowledged. The authors are grateful to the Director, National Bureau of Plant Genetic Resources, New Delhi 110012, India and Honourable Vice Chancellor of Sardar Vallabh Bhai Patel University of Agriculture and Technology, Meerut, 250110 (UP), India, for all kind support in research work.

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Authors and Affiliations

  1. Directorate of Research, Sardar Vallabh Bhai Patel University of Agriculture and Technology, Modipuram, Meerut, Uttar Pradesh, 250110, India

    Vipin Kumar & Amit Kumar Sharma

  2. Department of Fingerprinting, College of Biotechnology, Sardar Vallabh Bhai Patel University of Agriculture and Technology, Modipuram, Meerut, Uttar Pradesh, 250110, India

    Shailendra Sharma

  3. Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University (BHU), Varanasi, Uttar Pradesh, 221005, India

    Shiveta Sharma

  4. National Research Centre on DNA Fingerprinting, National Bureau of Plant Genetic Resources (NBPGR), New Delhi, 110012, India

    K. Venkataramana Bhat

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  1. Vipin Kumar
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  2. Shailendra Sharma
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  3. Amit Kumar Sharma
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Correspondence to Vipin Kumar.

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Kumar, V., Sharma, S., Sharma, A.K. et al. Comparative analysis of diversity based on morpho-agronomic traits and microsatellite markers in common bean. Euphytica 170, 249–262 (2009). https://doi.org/10.1007/s10681-009-9965-9

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