Abstract
Purpose
To test whether infection with select human polyomaviruses (HPyV) and human papillomaviruses (HPV) is associated with incident lung cancer.
Methods
We performed a nested case–control study, testing serum from the carotene and retinol efficacy trial, conducted 1985–2005, for antibodies to Merkel cell (MCV), KI (KIV), and WU (WUV) HPyVs as well as to six high-risk and two low-risk HPV types. Incident lung cancer cases (n = 200) were frequency-matched with controls (n = 200) on age, enrollment and blood draw dates, intervention arm assignment, and the number of serum freeze/thaw cycles. Sera were tested using multiplex liquid bead microarray antibody assays. We used logistic regression to assess the association between HPyV and HPV antibodies and lung cancer.
Results
There was no evidence of a positive association between levels of MCV, KIV, or WUV antibodies and incident lung cancer (p corrected >0.10 for all trend tests; odds ratio (OR) range 0.72–1.09, p corrected >0.10 for all). There was also no evidence for a positive association between HPV 16 or 18 infection and incident lung cancer (p corrected ≥0.10 for all trend tests; OR range 0.25–2.54, p > 0.05 for all OR > 1), but the number of persons with serologic evidence of these infections was small.
Conclusions
Prior infection with any of several types of HPyV or HPV was not associated with subsequent diagnosis of lung cancer. Infection with these viruses likely does not influence a person’s risk of lung cancer in Western smoking populations.
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Acknowledgments
We extend our gratitude to the study participants and CARET personnel who made this study possible. We also thank and acknowledge the contributions of Greg Wipf for assistance in the laboratory, and Margaret M. Madeleine, Lisa Johnson, and Alexa Resler for their consultation regarding study design and analytical methods. This work was supported by the Fogarty International Clinical Research Scholars and Fellows Program at Vanderbilt University [R24 TW007988 trainee support to D.V. Colombara]; the National Cancer Institute [R25 CA094880 trainee support to D.V. Colombara]; and the National Center for Advancing Translational Sciences of the National Institutes of Health [UL1TR000423 pilot award to L.E. Manhart and D.V. Colombara].
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LE Manhart has received test kits and reagents from Hologic/Gen-Probe over the past 5 years. JS Smith has received unrestricted educational grants, consultancy, and research grants from GlaxoSmithKline and Merck Corporation over the past 5 years. Y-L Qiao has received unrestricted educational grants, consultancy, and research grants from Qiagen, GlaxoSmithKline, and Merck Corporation over the past 5 years. All other authors report no potential conflicts.
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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
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Informed consent was obtained from all individual participants included in the study.
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Colombara, D.V., Manhart, L.E., Carter, J.J. et al. Prior human polyomavirus and papillomavirus infection and incident lung cancer: a nested case–control study. Cancer Causes Control 26, 1835–1844 (2015). https://doi.org/10.1007/s10552-015-0676-3
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DOI: https://doi.org/10.1007/s10552-015-0676-3