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The effect of cyclooxygenase (COX) inhibitors on Japanese medaka (Oryzias latipes) reproduction parameters fed with high level of arachidonic acid (20:4 n-6)

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Abstract

Broodstock of mature Japanese medaka (Oryzias latipes Temminck and Schlegel, 1846) (mean weight 0.45 ± 0.03 g; age 6 months) were maintained in glass aquaria (8 males and 8 females in each aquarium). The fish were fed commercial feed (group C) or this feed was supplemented with arachidonic acid ARA (2 % of total fatty acids in feed; group A) and one of the COX inhibitors: resveratrol (group AR) or NS-398 (group ANS) with amount 20 mg kg−1 BW day−1 of one of the COX inhibitors. The effect of 2 weeks of feeding experimental diets on reproductive parameters (fecundity, embryo and larval survival, hatching rate, and larvae body weight) was analyzed. Larvae (obtained from experimental dietary treatments) were reared for 2 weeks. The percent of spawning female and fecundity was similar in dietary treatments. COX inhibitors in broodstock diet caused a decrease in embryo survival rate. Eggs of medaka from group ANS and AR revealed the lowest hatchability rate. In contrast, group A revealed a higher hatchability rate at the same time. Larval body weight after hatching was significantly the highest in this group. At the end of the experiment, larvae body weight ranged from 1.62 ± 0.43 mg in group AN to 2.89 ± 0.74 mg in group A. These results show that the COX inhibitors decreased gamete and larval quality regardless of high levels of ARA in broodstock diet.

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References

  • Abi-Ayad S-MAE-A, Melard C, Kestemont P (1999) Effects of n-3 fatty acids in Eurasian perch broodstock diet on egg fatty acid composition and larvae stress resistance. Aquacult Int 5:161–168

    Google Scholar 

  • Bell JG, Sargent JR (2003) Arachidonic acid in aquaculture feeds: current status and future opportunities. Aquaculture 218:491–499

    Article  CAS  Google Scholar 

  • Bruce M, Oyen F, Bell G, Asturiano JF, Farndale B, Carrillo M, Zanuy S, Ramos J, Bromage N (1999) Development of broodstock diets for the European sea bass (Dicentrachus labrax) with special emphasis on the importance of n-3 and n-6 highly unsaturated fatty acids to reproductive performance. Aquaculture 177:85–97

    Article  CAS  Google Scholar 

  • Fernández–Palacios H, Izquierdo M, Robaina L, Valencia A, Salhi M, Vergara JM (1995) Effect of n-3 HUFA level in broodstock diets on egg quality of gilthead sea bream (Sparus aurata L.). Aquaculture 132:325–337

    Article  Google Scholar 

  • Flippin JL, Hugget D, Foran CM (2007) Changesin the timing of reproduction following chronic exposure to ibuprofen in medaka, Oryzias latipes. Aquat Toxicol 81:73–78

    Article  CAS  PubMed  Google Scholar 

  • Folch H, Less M, Stanley HA (1957) A simple method for isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–498

    CAS  PubMed  Google Scholar 

  • Fujimori Ch, Ogiwara K, Hagiwara A, Rajapakse S, Kimura A, Takahashi T (2011) Expression of cyclooxygenase-2 and prostaglandin receptor EP4b mRNA in the ovary of the medaka fish, Oryzias latipes: possible involvement in ovulation. Mol Cell Endocrinol 332:67–77

    Article  CAS  PubMed  Google Scholar 

  • Furuita H, Yamamoto T, Shima T, Suzuki N, Takeuchi T (2003) Effect of arachidinic acid levels in broodstock diet on larval and egg quality of Japanese flounder Paralichthys olivaceus. Aquaculture 220:725–735

    Article  CAS  Google Scholar 

  • Hashimoto S, Watanabe E, Ikeda M, Terao Y, Strussmann CA, Inoue M, Hara A (2009) Effects of ethinyl estradiol on medaka (Oryzias latipes) as measured by sperm motility and fertilization success. Arch Environ Contam Toxicol 56:253–259

    Article  CAS  PubMed  Google Scholar 

  • Havird JC, Miyamoto MM, Choe KP, Evans DH (2008) Gene duplications and losses within the cyclooxygenase family of teleosts and other chordates. Mol Biol Evol 25:2349–2359

    Article  CAS  PubMed  Google Scholar 

  • Hong HN, Kim HN, Park KS, Lee SK, Gu MB (2007) Analysis of the effects diclofenac has on Japanese medaka (Oryzias latipes) using real-time PCR. Aquat Toxicol 67:2115–2121

    CAS  Google Scholar 

  • Izoquiredo MS, Fernandez-Palacios H, Tacon AGJ (2001) Effect of broodstock nutrition on reproductive performance of fish. Aquaculture 197:25–42

    Article  Google Scholar 

  • Kinoshita M, Murata K, Naruse K, Tanaka M (2009) Medaka management. In: Kinoshita M, Murata K, Naruse K, Tanaka M (eds) Medaka. Biology, management, and experimental protocols. Wiley-Blackwell, USA, pp 31–65

    Chapter  Google Scholar 

  • Kjorsvik E, Mangorjensen A, Holmefjord I (1990) Egg quality in fishes. Adv Mar Biol 26:71–113

    Google Scholar 

  • Kowalska A, Kowalski RK, Zakęś Z (2011) The effect of selective cyclooxygenase (COX) inhibitors on Japanese medaka (Oryzias latipes) reproduction parameters. World Acad Sci Eng Technol 77:19–23

    Google Scholar 

  • Navas JM, Bruce M, Trush M, Farndale BM, Bromage N, Zanuy S, Carrillo M, Bell JG, Ramos J (1997) The impact of seasonal alteration in the lipid composition of broodstock diets on egg quality in the European sea bass. J Fish Biol 51:760–763

    Article  CAS  Google Scholar 

  • Pavlov D, Kjorsvik E, Refsti T, Andersen O (2004) Broodstock and egg production. In: Moksness E, Kjorsvik E, Olsen Y (eds) Culture of cold water marine fish. Blackwell Science, Oxford, pp 129–203

    Chapter  Google Scholar 

  • Petersen RK, Jorgensen C, Rustan AC, Froyland L, Muller-Decker K, Furstenberger G, Berge RK, Kristiansen K, Madsen L (2003) Arachidonic acid-dependent inhibition of adipocyte differentiation requires PKA activity and is associated with sustained expression of cyclooxygenases. J Lipid Res 44:2320–2330

    Google Scholar 

  • Sargent JR, Tocher DR, Bell JG (2002) The Lipids. In: Halver JE, Hardy R (eds) Fish nutrition. Academic Press, San Diego, pp 182–246

    Google Scholar 

  • Yang H, Tiersch TR (2009) Sperm motility initiation and duration in a euryhaline fish, medaka (Oryzias latipes). Theriogenology 72(3):386–392

    Article  CAS  PubMed Central  PubMed  Google Scholar 

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Acknowledgments

The authors wish to thank the Marek Hopko for his excellent technical assistance. Fish were originally supplied from National BioResource Project Medaka (NBRP Medaka) conducted by the National Institute of Natural Science (Japan). This study was funded by the Ministry of Science and Higher Education in the years 2009-2012 as research project no N N311 251137.

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  1. Department of Aquaculture, The Stanisław Sakowicz Inland Fisheries Institute, Olsztyn, Poland

    Agata Kowalska

  2. Department of Gamete and Embryo Biology, Polish Academy of Sciences, Olsztyn, Poland

    Radosław Kajetan Kowalski

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  1. Agata Kowalska
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  2. Radosław Kajetan Kowalski
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Correspondence to Radosław Kajetan Kowalski.

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Kowalska, A., Kowalski, R.K. The effect of cyclooxygenase (COX) inhibitors on Japanese medaka (Oryzias latipes) reproduction parameters fed with high level of arachidonic acid (20:4 n-6). Aquacult Int 22, 185–193 (2014). https://doi.org/10.1007/s10499-013-9684-z

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  • DOI: https://doi.org/10.1007/s10499-013-9684-z

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