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Inquisition of Microcystis aeruginosa and Synechocystis nanowires: characterization and modelling

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Abstract

Identification of extracellular conductive pilus-like structures (PLS) i.e. microbial nanowires has spurred great interest among scientists due to their potential applications in the fields of biogeochemistry, bioelectronics, bioremediation etc. Using conductive atomic force microscopy, we identified microbial nanowires in Microcystis aeruginosa PCC 7806 which is an aerobic, photosynthetic microorganism. We also confirmed the earlier finding that Synechocystis sp. PCC 6803 produces microbial nanowires. In contrast to the use of highly instrumented continuous flow reactors for Synechocystis reported earlier, we identified simple and optimum culture conditions which allow increased production of nanowires in both test cyanobacteria. Production of these nanowires in Synechocystis and Microcystis were found to be sensitive to the availability of carbon source and light intensity. These structures seem to be proteinaceous in nature and their diameter was found to be 4.5–7 and 8.5–11 nm in Synechocystis and M. aeruginosa, respectively. Characterization of Synechocystis nanowires by transmission electron microscopy and biochemical techniques confirmed that they are type IV pili (TFP) while nanowires in M. aeruginosa were found to be similar to an unnamed protein (GenBank : CAO90693.1). Modelling studies of the Synechocystis TFP subunit i.e. PilA1 indicated that strategically placed aromatic amino acids may be involved in electron transfer through these nanowires. This study identifies PLS from Microcystis which can act as nanowires and supports the earlier hypothesis that microbial nanowires are widespread in nature and play diverse roles.

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Acknowledgments

The authors thank The Energy and Resources Institute, India and Deakin University, Australia for providing financial support and necessary infrastructure to carry out planned research activities. Sandeep Sure is a recipient of a postgraduate scholarship offered by Deakin University, Australia.

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Authors and Affiliations

  1. TERI-Deakin Nanobiotechnology Centre, TERI Gram, The Energy and Resources Institute, Gual Pahari, Gurgaon Faridabad Road, Gurgaon, 122 001, Haryana, India

    Sandeep Sure, Aditya Gaur, Chandrakant Tripathi, Alok Adholeya & Mandira Kochar

  2. Centre for Cellular and Molecular Biology, Deakin University, 221 Burwood Highway, Burwood, Melbourne, VIC, 3125, Australia

    Angel A. J. Torriero & M. Leigh Ackland

  3. Institute for Frontier Materials, Deakin University, Geelong Waurn Ponds Campus, Geelong, VIC, 3216, Australia

    Lu Hua Li & Ying Chen

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  1. Sandeep Sure
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Correspondence to Mandira Kochar.

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Sure, S., Torriero, A.A.J., Gaur, A. et al. Inquisition of Microcystis aeruginosa and Synechocystis nanowires: characterization and modelling. Antonie van Leeuwenhoek 108, 1213–1225 (2015). https://doi.org/10.1007/s10482-015-0576-2

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