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Bradymyces gen. nov. (Chaetothyriales, Trichomeriaceae), a new ascomycete genus accommodating poorly differentiated melanized fungi

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Abstract

Three slow growing, melanized and morphologically poorly differentiated fungal strains were isolated from a hyperaemic focus near the enlarged spleen of a farmed rainbow trout (Oncorhynchus mykiss) and from a rock collected at 3,200 m a. s. l. (Alps, Italy). Two phylogenetic analyses of the combined nuc18S and nuc28S rDNA and ITS rDNA and β-tubulin sequences showed that these isolates belong to the Trichomeriaceae, a family of the ascomycete order Chaetothyriales containing black yeasts that cause infections in humans and animals. The strains form a well-supported monophyletic clade. The new genus Bradymyces, with two new species, Bradymyces oncorhynchi and Bradymyces alpinus, is proposed based on phylogenetic, ecophysiological and morphological data. It is characterized by the presence of moniliform hyphae, blastic proliferation, endoconidia, multicellular and muriform bodies, and bodies with dark fragmented incrustations on the surface. Bradymyces most closely resembles members of Knufia. The ex-type isolate of B. oncorhynchi CCF 4369T ( = CBS 133066T = CCFEE 6134T) represents the first case of a Trichomeriaceae member isolated from cold-blooded water vertebrates. B. alpinus [ex-type strain CCFEE 5493T ( = CBS 138368T = CCF 4803T)] is represented by two isolates from a single locality in the Alps and in contrast to B. oncorhynchi shows overall slower growth parameters and does not grow at 25 °C.

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Abbreviations

MLBS:

Maximum likelihood bootstrap support

PP:

Posterior probability

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Acknowledgments

This study was supported by the Ministry of Education, Youth and Sports (CZ.1.07/2.3.00/20.0055, CZ.1.07/2.3.00/30.0003 and SVV project), Ministry of Culture of the Czech Republic through institutional financing of long-term conceptual development of the research institution (the Silesian Museum, MK000100595), internal Grant of the Silesian Museum No. IGS 201402/2014, the Project of the National Foundation of the Czech Republic (GAP 506/12/0038) and as a long-term research development project of the Institute of Botany, Academy of Sciences No. RVO 67985939, and the Institute of Microbiology, Academy of Sciences No. RVO 61388971. Molecular genetics analyses were supported by the project GAUK 607812. MIUR-PRIN 2008 (prot. 2008AR8MX9) is gratefully acknowledged for financial support. The authors thank the PNRA (Italian National Program for Antarctic Research) and the Italian National Antarctic Museum “Felice Ippolito” for funding CCFEE (Culture Collection of Fungi From Extreme Environments). The Alpine guide A. Serafini is kindly acknowledged for collecting rock samples from the Alps. We thank Milada Chudíčková for her invaluable assistance in laboratory and Walter Gams for his helpful editorial suggestions.

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Authors and Affiliations

  1. Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01, Praha 2, Czech Republic

    Vit Hubka & Miroslav Kolařík

  2. Laboratory of Fungal Genetics and Metabolism, Institute of Microbiology of the AS CR, v. v. i., Vídeňská 1083, 142 20, Prague 4, Czech Republic

    Vit Hubka & Miroslav Kolařík

  3. Department of Taxonomy, Institute of Botany of the AS CR, v. v. i., 252 43, Průhonice, Czech Republic

    Martina Réblová

  4. Department of Zoology, Silesian Museum, Nádražní okruh 31, 746 01, Opava, Czech Republic

    Jiří Řehulka

  5. Department of Ecological and Biological Sciences (DEB), University of Tuscia, Largo dell’Università snc, 01100, Viterbo, Italy

    Laura Selbmann & Daniela Isola

  6. CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands

    Sybren G. de Hoog

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Hubka, V., Réblová, M., Řehulka, J. et al. Bradymyces gen. nov. (Chaetothyriales, Trichomeriaceae), a new ascomycete genus accommodating poorly differentiated melanized fungi. Antonie van Leeuwenhoek 106, 979–992 (2014). https://doi.org/10.1007/s10482-014-0267-4

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