Abstract
A newly isolated microbial strain of thermophilic genus Geobacillus has been described with emphasis on polyphasic characterization and its application for degradation of hydrogen peroxide. The validation of this thermophilic strain of genus Geobacillus designated as BSS-7 has been demonstrated by polyphasic taxonomy approaches through its morphological, biochemical, fatty acid methyl ester profile and 16S rDNA sequencing. This thermophilic species of Geobacillus exhibited growth at broad pH and temperature ranges coupled with production of extraordinarily high quantities of intracellular catalase, the latter of which as yet not been reported in any member of this genus. The isolated thermophilic bacterial culture BSS-7 exhibited resistance against a variety of organic solvents. The immobilized whole cells of the bacterium successfully demonstrated the degradation of hydrogen peroxide (H2O2) in a packed bed reactor. This strain has potential application in various analytical and diagnostic methods in the form of biosensors and biomarkers in addition to applications in the textile, paper, food and pharmaceutical industries.
Similar content being viewed by others
References
Fruhwirth GO, Paar A, Gudelj M, Cavaco-Paulo A, Robra KH, Gubitz GM (2002) An immobilised catalase peroxidase from the alkalothermophilic Bacillus SF for the treatment of textile-bleaching effluents. Appl Microbiol Biotechnol 60(3):313–319
Hillenbrand T, Bohm E, Landwehr M, Marscheider-Weidemann F (1999) Die Abwassersituation in der deutschen Papier-Textil- und Lederindustrie. Gwf Wasser Abwasser 140(4):1–12
Rach JJ, Schreier TM, Howe GE, Redman SD (1997) Effect of species, life stage, and water temperature on the toxicity of hydrogen peroxide to fish. Prog Fish Cult 59(1):41–46
Xenopoulos MA, Bird DF (1997) Effect of acute exposure to hydrogen peroxide on the production of phytoplankton and bacterioplankton in a Mesohumic Lake. Photochem Photobiol 66(4):471–478
Paar A, Costa S, Tzanov T, Gudelj M, Robra KH, Cavaco-Paulo A, Gubitz GM (2001) Thermoalkalistable catalases from newly isolated Bacillus sp. for the treatment and recycling of textile bleaching effluents. J Biotechnol 8:147–153
Sooch BS, Kauldhar BS, Puri M (2014) Recent insights into microbial catalases: isolation, production and purification. Biotechnol Adv 32(8):1429–1447
Spiro MC, Griffith WP (1997) The mechanism of hydrogen peroxide bleaching. Text Chem Color 29(11):12–13
Costa SA, Tzanov T, Paar A, Gudelj M, Gubitz GM, Cavaco-Paulo A (2001) Immobilization of catalases from Bacillus SF on alumina for the treatment of textile bleaching effluents. Enzym Microb Technol 28:815–819
Deep K, Poddar A, Das SK (2013) Anoxybacillus suryakundensis sp. nov., a moderately thermophilic, alkalitolerant bacterium isolated from hot spring at Jharkhand, India. Plos One 8(12):e85493/1–11
Zhu H, Guo J, Chen M, Feng G, Yao Q (2012) Burkholderia dabaoshanensis sp. nov., a heavy-metal tolerant bacteria isolated from Dabaoshan mining area soil in China. Plos One 7(12):e50225/1–6
Gudelj M, Fruhwirth GO, Paar A, Lottspeich F, Robra KH, Cavaco-Paulo A (2001) A catalase-peroxidase from a newly isolated thermoalkaliphilic Bacillus sp. with potential for the treatment of textile bleaching effluents. Extremophiles 5(6):423–429
Nishikawa M, Hyoudou K, Kobayashi Y, Umeyama Y, Takakura Y, Hashida M (2005) Inhibition of metastatic tumor growth by targeted delivery of antioxidant enzymes. J Control Release 109(1–3):101–107
Burg B (2003) Extremophiles as a source for novel enzymes. Curr Opin Microbiol 6:213–218
Ishida M, Yoshida M, Oshima T (1997) Highly efficient production of enzymes of an extreme thermophile, Thermus thermophilus: a practical method to over express GC-rich genes in Escherichia coli. Extremophiles 1:157–162
Demirjian DC, Moris-Varas F, Cassidy CS (2001) Enzymes from extremophiles. Curr Opin Chem Biol 5:144–151
Coombs JM, Brenchley JE (1999) Biochemical and phylogenetic analyses of a cold-active β-galactosidase from the lactic acid bacterium Carnobacterium piscicola. Appl Environ Microbiol 65:5443–5450
Madigan MT, Marrs BL (1997) Extremophiles. Sci Am 276:66–71
Schiraldi C, De Rosa M (2002) The production of biocatalysts and biomolecules from extremophiles. Trends Biotechnol 20(12):515–521
Somashekar D, Venkateshwaran G, Agrawal R, Prakash MH, Basappa SC (1999) Novel enrichment technique for the isolation of highly potent catalase producing yeasts from soil. Biotechnol Tech 13:65–68
Sinha AK (1972) Colorimetric assay of catalase. Anal Biochem 47(2):389–394
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA 6.06: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729
Kunitsky C, Osterhout G, Sasser M (2005) Identification of microorganisms using fatty acid methyl ester (FAME) analysis and the MIDI Sherlock® Microbial Identification System. In: Miller MJ (ed) Encyclopedia of Rapid Microbiological Methods. Davis Healthcare International Publishing, p 1–17
Prescott LM, Harley JP, Klein DA (2002) Microbial growth. Microbiology, 5th edn. The McGraw-Hill companies, New York, pp 113–135
Neumann G, Veeranagouda Y, Karegoudar T, Sahin O, Mausezahl I, Kabelitz N (2005) Cells of Pseudomonas putida and Enterobacter sp. adapt to toxic organic compounds by increasing their size. Extremophiles 9:163–168
Puri M, Kaur A, Singh RS, Schwarz WH, Kaur A (2010) One step purification and immobilization of His-tagged rhamnosidase for naringin hydrolysis. Process Biochem 45(4):451–456
Manachini PL, Mora D, Nicastro G, Parini C, Stackebrandt E, Pukall R (2000) Bacillus thermodenitrificans sp. nov., nom. rev. Int J Syst Evol Microbiol 50:1331–1337
Nazina TN, Lebedeva EV, Poltaraus AB, Tourova TP, Grigoryan AA, Sokolova DS (2004) Geobacillus gargensis sp. nov., a novel thermophile from a hot spring, and the reclassification of Bacillus vulcani as Geobacillus vulcani comb. nov. Int J Syst Evol Microbiol 54:2019–2024
Jukes TH, Cantor CR (1969) Evolution of protein molecules. In: Munro HN (ed) Mammalian protein metabolism. Academic Press, New York, pp 21–132
Nazina TN, Tourova TP, Poltaraus AB, Novikova EV, Grigoryan AA, Ivanova AE (2001) Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov., and Geobacillus uzenensis sp. nov., from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans. Int J Syst Evol Microbiol 51:433–446
Deflaun MF, Fredrickson JK, Dong H, Pfiffner SM, Onstott TC (2007) Isolation and characterization of a Geobacillus thermoleovorans strain from an ultra-deep South African gold mine. Syst Appl Microbiol 30(2):152–164
Coorevits A, Dinsdale AE, Halket G, Lebbe L, Vos PD, Landschoot AV (2012) Taxonomic revision of the genus Geobacillus: emendation of Geobacillus, G. stearothermophilus, G. jurassicus, G. toebii, G. thermodenitrificans and G. thermoglucosidans (nom. corrig., formerly ‘thermoglucosidasius’); transfer of Bacillus thermantarcticus to the genus as G. thermoantarcticus comb. nov.; proposal of Caldibacillus debilis gen. nov., comb. nov.; transfer of G. tepidamans to Anoxybacillus as A. tepidamans comb. nov.; and proposal of Anoxybacillus caldiproteolyticus sp. nov. Int J Syst Evol Microbiol 62:1470–1485
Logan NA, Vos PD, Dinsdale A (2009) Genus Geobacillus Nazina et al. 2001. In: Vos PD, Garrity G, Jones D, Krieg NR, Ludwig WF, Rainey A, Schleifer KH, Whitman WB (eds) Bergey’s manual of systematic bacteriology. Springer, New York, pp 144–160
Kobayashi I, Tamura T, Sghaier H, Narumi I, Yamaguchi S, Umeda K (2006) Characterization of monofunctional catalase KatA from radioresistant bacterium Deinococcus radiodurans. J Biosci Bioeng 101(4):315–321
Timucin E, Sezerman OU (2013) The conserved lid tryptophan, W211, potentiates thermostability and thermoactivity in bacterial thermoalkalophilic lipases. Plos One 8(12):e85186/1–17
Srivastava A, Sinha S (2014) Thermostability of in vitro evolved Bacillus subtilis lipase A: a network and dynamics perspective. Plos one 9(8):e102856/1–14
Sooch BS, Kauldhar BS (2013) Influence of multiple bioprocess parameters on production of lipase from Pseudomonas sp. BWS-5. Braz Arch Biol Technol 56(5):711–721
Fortina MG, Mora D, Schumann P, Parini C, Manachini PL, Stackebrandt E et al (2001) Reclassification of Saccharococcus caldoxylosilyticus as Geobacillus caldoxylosilyticus (Ahmad, 2000) comb. nov. Int J Syst Evol Microbiol 51:2063–2071
Krulwich TA, Guffanti AA, Seto-Young D (1990) pH homeostasis and bio-energetic work in alkalophiles. FEMS Microbiol Rev 75:271–278
Klibanov AM (2001) Improving enzymes by using them in organic solvents. Nature 409:241–246
Albers SV, Driessen AJ (2008) Conditions for gene disruption by homologous recombination of exogenous DNA into the Sulfolobus solfataricus genome. Archaea 2(3):145–149
Siristova L, Melzoch K, Rezanka T (2009) Fatty acids, unusual glycophospholipids and DNA analyses of thermophilic bacteria isolated from hot springs. Extremophiles 13(1):101–109
Yang YL, Yang FL, Jao SC, Chen MY, Tsay SS, Zou W, Wu SH (2006) Structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles Thermus and Meiothermus. J Lipid Res 47(8):1823–1832
Sung MH, Kim H, Bae JW, Rhee SK, Jeon CO, Kim K (2002) Geobacillus toebii sp. nov., a novel thermophilic bacterium isolated from hay compost. Int J Syst Evol Microbiol 52:2251–2255
Ahmad S, Scopes RK, Rees GN, Patel BKC (2000) Saccharococcus caldoxylosilyticus sp. nov., an obligately thermophilic, xylose-utilizing, endospore-forming bacterium. Int J Syst Evol Microbiol 50:517–523
Oluoch KR, Welander U, Andersson MM, Mulaa FJ, Mattiasson B, Hatti-Kaul R (2006) Hydrogen peroxide degradation by immobilized cells of alkaliphilic Bacillus halodurans. Biocatal Biotransform 24(3):215–222
Sooch BS, Kauldhar BS (2015) Development of an eco-friendly whole cell based continuous system for the degradation of hydrogen peroxide. J Bioprocess Biotech 5(6):1–5
Claus D, Berkeley RCW (1986) Genus Bacillus Cohn 1872. In: Sneath PHA, Mair NS, Sharpe ME, Holt JG (eds) Bergey’s manual of systematic bacteriology. Williams & Wilkins, Baltimore, pp 1105–1139
Priest FG, Goodfellow M, Todd C (1988) A numerical classification of the genus Bacillus. J Gen Microbiol 134:1847–1882
Suzuki Y, Kishigami T, Inoue K, Mizoguchi Y, Eto N, Takagi M (1983) Bacillus thermoglucosidasius sp. nov., a new species of obligately thermophilic bacilli. Syst Appl Microbiol 4:487–495
White D, Sharp RJ, Priest FG (1993) A polyphasic taxonomic study of thermophilic bacilli from a wide geographical area. Antonie Van Leeuwenhoek 64:357–386
Caccamo D, Gugliandolo C, Stackebrandt E, Maugeri TL (2000) Bacillus vulcani sp. nov., a novel thermophilic species isolated from a shallow marine hydrothermal vent. Int J Syst Evol Microbiol 50:2009–2012
Nazina TN, Sokolovaa DS, Grigoryana AA, Shestakovaa NM, Mikhailovaa EM, Poltarausb AB (2005) Geobacillus jurassicus sp. nov., a new thermophilic bacterium isolated from a high-temperature petroleum reservoir, and the validation of the Geobacillus species. Syst Appl Microbiol 28:43–53
Acknowledgments
The authors are thankful to Department of Biotechnology, Punjabi University, Patiala, for providing necessary laboratory facilities. The authors also thank University Grants Commission, New Delhi, India, for providing Rajiv Gandhi National Fellowship for doctoral studies to Mr. Baljinder Singh Kauldhar to execute his research work. Facilities availed for the validation of present studies from National Institute for Pharmaceutical and Drug Research, Mohali, India, for SEM & TEM micrographs are highly acknowledged.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest in the present publication.
Rights and permissions
About this article
Cite this article
Sooch, B.S., Kauldhar, B.S. & Puri, M. Isolation and polyphasic characterization of a novel hyper catalase producing thermophilic bacterium for the degradation of hydrogen peroxide. Bioprocess Biosyst Eng 39, 1759–1773 (2016). https://doi.org/10.1007/s00449-016-1651-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00449-016-1651-4