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Ion transport in broad bean leaf mesophyll under saline conditions

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Abstract

Main conclusion

Salt stress reduces the ability of mesophyll tissue to respond to light. Potassium outward rectifying channels are responsible for 84 % of Na + induced potassium efflux from mesophyll cells.

Modulation in ion transport of broad bean (Vicia faba L.) mesophyll to light under increased apoplastic salinity stress was investigated using vibrating ion-selective microelectrodes (the MIFE technique). Increased apoplastic Na+ significantly affected mesophyll cells ability to respond to light by modulating ion transport across their membranes. Elevated apoplastic Na+ also induced a significant K+ efflux from mesophyll tissue. This efflux was mediated predominately by potassium outward rectifying channels (84 %) and the remainder of the efflux was through non-selective cation channels. NaCl treatment resulted in a reduction in photosystem II efficiency in a dose- and time-dependent manner. In particular, reductions in Fv′/Fm′ were linked to K+ homeostasis in the mesophyll tissue. Increased apoplastic Na+ concentrations induced vanadate-sensitive net H+ efflux, presumably mediated by the plasma membrane H+-ATPase. It is concluded that the observed pump’s activation is essential for the maintenance of membrane potential and ion homeostasis in the cytoplasm of mesophyll under salt stress.

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Abbreviations

DMSO:

Dimethyl sulfoxide

KOR:

Potassium outward rectifying channels

NHX:

Sodium/proton exchanger

NSCC:

Non-selective cation channels

PCD:

Programmed cell death

ROS:

Reactive oxygen species

TEA:

Tetraethylammonium

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Acknowledgments

This work was supported by the Australian Research Council grant DP0987402 to SS and RMJ and Grain Research and Development Corporation (GRDC) grant to SS.

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Authors and Affiliations

  1. School of Land and Food, University of Tasmania, Private Bag 54, Hobart, TAS, 7001, Australia

    William J. Percey, Lana Shabala, Jayakumar Bose & Sergey Shabala

  2. Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, University of Tasmania, Hobart, TAS, 7001, Australia

    Michael C. Breadmore

  3. School of Pharmacy, University of Tasmania, Hobart, TAS, 7001, Australia

    Rosanne M. Guijt

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  1. William J. Percey
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  2. Lana Shabala
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  5. Jayakumar Bose
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  6. Sergey Shabala
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Correspondence to Sergey Shabala.

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425_2014_2117_MOESM1_ESM.pptx

Suppl. Fig. S1 a Relationship between net H+ efflux and net K+ flux measured from leaf mesophyll from 15 min after treatment with 100 mM NaCl (a) and 20 mM NaCl (b). One (of six) representative examples is shown for each treatmentSuppl. Fig. S2 Rhythmical net H+ flux response to light/dark fluctuation measured from mesophyll tissues exposed to a range of salinities for 72 h. One (of 6-8) typical examples is shownSuppl. Fig. S3 Rhythmical net K+ flux response to light/dark fluctuation measured from mesophyll tissues exposed to a range of salinities for 72 h. One (of 6-8) typical examples is shown (PPTX 172 kb)

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Percey, W.J., Shabala, L., Breadmore, M.C. et al. Ion transport in broad bean leaf mesophyll under saline conditions. Planta 240, 729–743 (2014). https://doi.org/10.1007/s00425-014-2117-z

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