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Regulation of the STARS signaling pathway in response to endurance and resistance exercise and training

  • Muscle Physiology
  • Published:
Pflügers Archiv - European Journal of Physiology Aims and scope Submit manuscript

Abstract

The striated muscle activator of Rho signaling (STARS) protein and members of its downstream signaling pathway, including myocardin-related transcription factor-A (MRTF-A) and SRF, are increased in response to prolonged resistance exercise training but also following a single bout of endurance cycling. The aim of the present study was to measure and compare the regulation of STARS, MRTF-A and SRF mRNA and protein following 10 weeks of endurance training (ET) versus resistance training (RT), as well as before and following a single bout of endurance (EE) versus resistance exercise (RE). Following prolonged training, STARS, MRTF-A and SRF mRNA levels were all increased by similar magnitude, irrespective of training type. In the training-habituated state, STARS mRNA increased following a single-bout RE when measured 2.5 and 5 h post-exercise and had returned to resting level by 22 h following exercise. MRTF-A and SRF mRNA levels were decreased by 2.5, 5, and 22 h following a single bout of RE and EE exercise when compared to their respective basal levels, with no significant difference seen between the groups at any of the time points. No changes in protein levels were observed following the two modes of exercise training or a single bout of exercise. This study demonstrates that the stress signals elicited by ET and RT result in a comparable regulation of members of the STARS pathway. In contrast, a single bout of EE and RE, performed in the trained state, elicit different responses. These observations suggest that in the trained state, the acute regulation of the STARS pathway following EE or RE may be responsible for exercise-specific muscle adaptations.

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Acknowledgments

Séverine Lamon was supported by an Alfred Deakin postdoctoral fellowship from Deakin University. Kristian Vissing was supported by the Novo Nordisk Foundation.

Conflict of interest

We state that there is no conflict of interest.

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Authors and Affiliations

  1. Centre for Physical Activity and Nutrition, School of Exercise and Nutrition Sciences, Deakin University, Burwood, Australia

    Séverine Lamon, Marita A. Wallace, Renae J. Stefanetti & Aaron P. Russell

  2. Section of Sport Science, Department of Public Health, Aarhus University, Dalgas Avenue 4, 8000, Aarhus, Denmark

    Stine K. Rahbek & Kristian Vissing

  3. Department of Internal Medicine and Endocrinology, Aarhus University Hospital, 8000, Aarhus, Denmark

    Mikkel H. Vendelbo

Authors
  1. Séverine Lamon
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  2. Marita A. Wallace
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  3. Renae J. Stefanetti
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  4. Stine K. Rahbek
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  5. Mikkel H. Vendelbo
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  6. Aaron P. Russell
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  7. Kristian Vissing
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Correspondence to Kristian Vissing.

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Lamon, S., Wallace, M.A., Stefanetti, R.J. et al. Regulation of the STARS signaling pathway in response to endurance and resistance exercise and training. Pflugers Arch - Eur J Physiol 465, 1317–1325 (2013). https://doi.org/10.1007/s00424-013-1265-5

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  • DOI: https://doi.org/10.1007/s00424-013-1265-5

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