Interactions among endocrinology, seasonal reproductive cycles and the nesting biology of the female green sea turtle

Abstract.

We collected data on plasma levels of testosterone+5a-dihydrotestosterone (T+DHT) and corticosterone (CORT) from adult female green sea turtles (Chelonia mydas) from southern Queensland during distinct stages of their reproductive cycle. Those females capable of breeding in a given year had elevated plasma steroid levels (T+DHT 0.91±0.08; CORT 1.05±0.29 ng/ml), associated with follicular development, until courtship began in October. At the beginning of the nesting season in November plasma levels of CORT were related to when the female first nested (r ²=0.06; F=10.45; P=0.01). However, they were not correlated with the number of clutches a female laid in that season (F=3.65; P=0.08). We repeatedly sampled 23 turtles over the nesting season and profiled changes in steroids immediately following oviposition of each clutch. Levels of T+DHT (range 0.41–0.58 ng/ml) and CORT (range 2.13–2.81 ng/ml) were similar through the early stages of the nesting season and inter-nesting period, and declined to near basal levels (T+DHT 0.37±0.03 and CORT 1.85±ng/ml) following the last clutch for the season. Steroid hormone levels were also low (T+DHT 0.38±0.16; CORT 0.46±0.21 ng/ml) in four independent post-breeding (atretic) females; samples for these females were taken at a time when body condition was presumably at the lowest for the season. Subtle changes in the nesting environment, such as variation in nesting habitat or the time of night that nesting occurred, were associated with a small and slow CORT increase. We suggest CORT is increased in nesting females to assist in lipid transfer to prepare the ovarian follicles and/or the reproductive organs for ovulation.