Skip to main content
SpringerLink
Log in

Comprehensive two-dimensional liquid chromatography

  • Review
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

Having nearly exhausted the possibilities for generating peak capacity through improvements in column technology, chromatographers are increasingly looking to alternative ways of maximising chromatographic separation. In recent years there has been increasing activity in the field of comprehensive multidimensional separations to meet analysis demands. Comprehensive two-dimensional liquid chromatography (LC×LC) approaches offer high peak capacity which leads to significantly improved analytical performance over single-column liquid chromatography. There are several closely related avenues available for achieving an LC×LC separation and this review pays special attention to the different valve-based interfaces that have been used to comprehensively couple the first and second dimension columns in LC×LC systems. A brief discussion of column choices for selected applications and the conditions employed is also presented.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11
Fig. 12
Fig. 13

Similar content being viewed by others

References

  1. Evans CR, Jorgenson JW (2004) Anal Bioanal Chem 378:1952–1961

    Article  CAS  Google Scholar 

  2. Giddings JC (1990) In: Cortes HJ (ed) Multidimensional chromatography. Marcel Dekker, New York, pp 1–27

    Google Scholar 

  3. Neue UD (2005) J Chromatogr A 1079:153–161

    Article  CAS  Google Scholar 

  4. Swartz ME (2005) LC-GC North Am Suppl 8–14

  5. Liu Z, Patterson DG Jr, Lee ML (1995) Anal Chem 67:3840–3845

    Article  CAS  Google Scholar 

  6. Schoenmakers P, Marriott P, Beens J (2003) LC-GC Europe 16:335–336 338–339

    CAS  Google Scholar 

  7. Murphy RE, Schure MR, Foley JP (1998) Anal Chem 70:1585–1594

    Article  CAS  Google Scholar 

  8. Schure MR (1997) J Microcol Sep 9:169–176

    Article  CAS  Google Scholar 

  9. Erni F, Frei RW (1978) J Chromatogr 149:561–569

    Article  CAS  Google Scholar 

  10. Bushey MM, Jorgenson JW (1990) Anal Chem 62:161–167

    Article  CAS  Google Scholar 

  11. Opiteck GJ, Lewis KC, Jorgenson JW, Anderegg RJ (1997) Anal Chem 69:1518–1524

    Article  CAS  Google Scholar 

  12. Chen X, Kong L, Su X, Fu H, Ni J, Zhao R, Zou H (2004) J Chromatogr A 1040:169–178

    Article  CAS  Google Scholar 

  13. Murphy RE, Schure MR, Foley JP (1998) Anal Chem 70:4353–4360

    Article  CAS  Google Scholar 

  14. Holland LA, Jorgenson JW (1995) Anal Chem 67:3275–3283

    Article  CAS  Google Scholar 

  15. Holland LA, Jorgenson JW (2000) J Microcol Sep 12:371–377

    Article  CAS  Google Scholar 

  16. van der Horst A, Schoenmakers PJ (2003) J Chromatogr A 1000:693–709

    Article  Google Scholar 

  17. 9th International Symposium on Hyphenated Techniques in Chromatography and Hyphenated Chromatographic Analyzers. February 8th–10th, 2006, York, UK

  18. Köhne AP, Welsch T (1999) J Chromatogr A 845:463–469

    Article  Google Scholar 

  19. Gray MJ, Dennis GR, Slonecker PJ, Shalliker RA (2004) J Chromatogr A 1041:101–110

    Article  CAS  Google Scholar 

  20. Opiteck GJ, Ramirez SM, Jorgenson JW, Moseley MA III (1998) Anal Biochem 258:349–361

    Article  CAS  Google Scholar 

  21. Opiteck GJ, Jorgenson JW, Moseley MA III, Anderegg RJ (1998) J Microcol Sep 10:365–375

    Article  CAS  Google Scholar 

  22. Venkatramani CJ, Zelechonok Y (2003) Anal Chem 75:3484–3494

    Article  CAS  Google Scholar 

  23. Svec F (2004) J Sep Sci 27:747–766

    Article  CAS  Google Scholar 

  24. Ikegami T, Tanaka N (2004) Curr Opin Chem Biol 8:527–533

    Article  CAS  Google Scholar 

  25. Ikegami T, Hara T, Kimura H, Kobayashi H, Hosoya K, Cabrera K, Tanaka N (2006) J Chromatogr A 1106:112–117

    Article  CAS  Google Scholar 

  26. Hu L, Chen X, Kong L, Su X, Ye M, Zou H (2005) J Chromatogr A 1092:191–198

    Article  CAS  Google Scholar 

  27. Dugo P, Favoino O, Luppino R, Dugo G, Mondello L (2004) Anal Chem 76:2525–2530

    Article  CAS  Google Scholar 

  28. Cortes HJ (1992) J Chromatogr 626:3

    Article  CAS  Google Scholar 

  29. Mondello L, Tranchida PQ, Stanek V, Jandera P, Dugo G, Dugo P (2005) J Chromatogr A 1086:91–98

    Article  CAS  Google Scholar 

  30. Giddings JC (1995) J Chromatogr A 703:3–15

    Article  CAS  Google Scholar 

  31. Ledford EB Jr, Phillips JB, Xu J, Gaines RB, Blomberg J (1996) Am Lab 14:22–25

    Google Scholar 

  32. Im K, Kim Y, Chang T, Lee K, Choi N (2006) J Chromatogr A 1103:235–242

    Article  CAS  Google Scholar 

  33. Coulier L, Kaal ER, Hankemeier T (2005) J Chromatogr A 1070:79–87

    Article  CAS  Google Scholar 

  34. Mondello L. 9th International Symposium on Hyphenated Techniques in Chromatography and Hyphenated Chromatographic Analyzers, February 8th–10th, 2006, York, UK

  35. Wolters DA, Washburn MP, Yates JR (2001) Anal Chem 73:5683–5690

    Article  CAS  Google Scholar 

  36. Tanaka N, Kimura H, Tokuda D, Hosoya K, Ikegami T, Ishizuka N, Minakuchi H, Nakanishi K, Shintani Y, Furuno M, Cabrera K (2004) Anal Chem 76:1273–1281

    Article  CAS  Google Scholar 

Download references

Acknowledgement

This work was supported under the Australian Research Council’s Discovery funding scheme (project number DP0663781).

Author information

Authors and Affiliations

  1. Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, University of Tasmania, Private Bag 75, Hobart, 7001, Australia

    Robert A. Shellie & Paul R. Haddad

Authors
  1. Robert A. Shellie
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Paul R. Haddad
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Robert A. Shellie.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shellie, R.A., Haddad, P.R. Comprehensive two-dimensional liquid chromatography. Anal Bioanal Chem 386, 405–415 (2006). https://doi.org/10.1007/s00216-006-0516-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-006-0516-0

Keywords

Search

Navigation