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Phylogenetic analysis of ten black yeast species using nuclear small subunit rRNA gene sequences

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Abstract

The nuclear small subunit rRNA genes of authentic strains of the black yeastsExophiala dermatitidis, Wangiella dermatitidis, Sarcinomyces phaeomuriformis, Capronia mansonii, Nadsoniella nigra var.hesuelica, Phaeoannellomyces elegans, Phaeococcomyces exophialae, Exophiala jeanselmei var.jeanselmei andE. castellanii were amplified by PCR and directly sequenced. A putative secondary structure of the nuclear small subunit rRNA ofExophiala dermatitidis was predicted from the sequence data. Alignment with corresponding sequences fromNeurospora crassa andAureobasidium pullulans was performed and a phylogenetic tree was constructed using the neighbor-joining method. The obtained topology of the tree was confirmed by bootstrap analysis. Based upon this analysis all fungi studied formed a well-supported monophyletic group clustering as a sister group to one group of the Plectomycetes (Trichocomaceae and Onygenales). The analysis confirmed the close relationship postulated betweenExophiala dermatitidis, Wangiella dermatitidis andSarcinomyces phaeomuriformis. This monophyletic clade also contains the teleomorph speciesCapronia mansonii thus confirming the concept of a teleomorph connection of the genusExophiala to a member of the Herpotrichiellaceae. However,Exophiala castellanii did not belong to this clade. Therefore, this species is not the anamorph ofCapronia mansonii as it was postulated.

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Authors and Affiliations

  1. Institute for Medical Microbiology, Klinikum RWTH Aachen, D-52057, Aachen, Germany

    G. Haase, L. Sonntag, A. Podbielski & B. Melzer-Krick

  2. Department of Biochemistry, Universiteit Antwerpen, Universiteitsplein 1, B-2610, Antwerp, Belgium

    Y. van de Peer

  3. Centraalbureau voor Schimmelcultures, P.O. Box 273, NL-3740 AG, Baarn, The Netherlands

    J. M. J. Uijthof

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  1. G. Haase
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  2. L. Sonntag
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  3. Y. van de Peer
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  4. J. M. J. Uijthof
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  5. A. Podbielski
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  6. B. Melzer-Krick
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Haase, G., Sonntag, L., van de Peer, Y. et al. Phylogenetic analysis of ten black yeast species using nuclear small subunit rRNA gene sequences. Antonie van Leeuwenhoek 68, 19–33 (1995). https://doi.org/10.1007/BF00873289

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