Skip to main content
SpringerLink
Log in

Secretion of β-lactamase from K. lactis using pEPS1, a convenient episomal vector designed for the secretion of foreign proteins

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

A convenient shuttle vector that enables high level secretion of proteins from Kluyveromyces lactis has been developed. The vector, pEPS1, contains a unique cloning site that allows the construction, in a single ligation step, of episomal plasmids capable of directing secretion of foreign gene products from K. lactis. As an example we demonstrate the production of β-lactamase and determine optimal conditions for its secretion into the culture media.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Becker, D.M., and Guarente, L. (1991) Meth. Enzymol. 194, 182–187.

    Google Scholar 

  • Bianchi, M.M., Falcone, C., Chen, X.J., Wesolowski-Louvel, M., Frontali, L., and Fukuhara, H. (1987) Curr. Genet., 12, 185–192.

    Google Scholar 

  • Bielefeld, M., and Hollenberg, C.P. (1992) Curr. Genet., 21, 265–268.

    Google Scholar 

  • Buckholz, R.G., and Gleeson, M.A.G. (1991) Bio/Technology, 9, 1067–1072.

    Google Scholar 

  • Fleer, R., Chen, X.J., Amellal, N., Yeh, P., Fournier, A., Guinet, F., Gault, N., Faucher, D., Folliard, F., Fukuhara, H., and Mayaux, J-F. (1991a) Gene, 107, 285–295.

    Google Scholar 

  • Fleer, R., Yeh, P., Amellal, N., Maury, I., Fournier, A., Bacchetta, F., Baduel, P., Jung, G., L'Hote, H., Becquart, J., Fukuhara, H., and Mayaux, J-F. (1991b) Bio/Technology, 9. 968–975.

    Google Scholar 

  • Naglak, T.J., and Wang, H.Y. (1990) Enzyme Microb. Technol., 12, 603–611.

    Google Scholar 

  • Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning. A laboratory manual (2nd Edn.) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

    Google Scholar 

  • Sutcliffe, J.G. (1978) Proc. Natl. Acad. Sci. USA, 75, 3737–3741.

    Google Scholar 

  • Stark, M.J.R., Boyd, A., Mileham, A.J., and Romanos, M.A. (1990) Yeast, 6, 1–29.

    Google Scholar 

  • van den Berg, J.A., van der Laken, K.J., van Ooyen, A.J.J., Renniers, T.C.H.M., Rietveld, K., Schaap, A., Brake, A.J., Bishop, R.J., Schultz, K., Moyer, D., Richman, M., and Shuster, J.R. (1990) Bio/Technology, 8, 135–139.

    Google Scholar 

  • Wesolowski-Louvel, M., Tanguy-Rougeau, C., and Fukuhara, H. (1988) Yeast, 4, 71–81.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biomolecular Research Institute, 343 Royal Parade, 3052, Parkville, Victoria, Australia

    I. G. Macreadie, L. A. Castelli, A. C. Ward, M. J. R. Stark & A. A. Azad

  2. Department of Biochemistry, The University, DD1 4HN, Dundee, UK

    M. J. R. Stark

Authors
  1. I. G. Macreadie
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. A. Castelli
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. C. Ward
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. J. R. Stark
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. A. Azad
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Macreadie, I.G., Castelli, L.A., Ward, A.C. et al. Secretion of β-lactamase from K. lactis using pEPS1, a convenient episomal vector designed for the secretion of foreign proteins. Biotechnol Lett 15, 213–218 (1993). https://doi.org/10.1007/BF00128307

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00128307

Keywords

Search

Navigation