Overview
- Explains difficulties in structural biology and provides methods to overcome these difficulties with protocols and theories behind them
- Offers not only experimental methods (protocols and examples of protein experiments) but also the theoretical background of each method.
- Includes abundant references and practical examples with useful figures
Part of the book series: Springer Protocols Handbooks (SPH)
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Table of contents (17 protocols)
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Front Matter
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Purification and Crystallization of Membrane Proteins
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Front Matter
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Crystallization and Crystal Engineering
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Front Matter
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Interaction Analysis
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Front Matter
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Advanced Methods for Structural Analyses
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Front Matter
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Keywords
About this book
This book provides practical information on a whole set of protein experiments for advanced structural biology, such as X-ray crystallography, NMR, electron microscopy, advanced mass spectroscopy, and surface plasmon resonance, as well as a wide variety of expression systems including eukaryotic and in vitro expression. In the past decade, structural genomics studies have pushed forward the development of automated methods in the field of structural biology, however there is an increasing need for the structural analysis of difficult targets, such as large protein complexes and membrane proteins, which are hard to achieve using conventional automated methods, and require knowledge that goes beyond standard protein chemistry protocols. To address these problems and to help researchers develop novel methods, this volume provides examples of the development of new protein investigation methods and their theoretical background.This book particularly appeals to graduate students, postdoctoral researchers, young investigators wishing to gain a better understanding of the theory behind experiments, and those seeking further advanced, practical structural biology methods.
Editors and Affiliations
Bibliographic Information
Book Title: Advanced Methods in Structural Biology
Editors: Toshiya Senda, Katsumi Maenaka
Series Title: Springer Protocols Handbooks
DOI: https://doi.org/10.1007/978-4-431-56030-2
Publisher: Springer Tokyo
eBook Packages: Springer Protocols
Copyright Information: Springer Japan 2016
Hardcover ISBN: 978-4-431-56028-9Published: 03 August 2016
Softcover ISBN: 978-4-431-56758-5Published: 07 June 2018
eBook ISBN: 978-4-431-56030-2Published: 20 July 2016
Series ISSN: 1949-2448
Series E-ISSN: 1949-2456
Edition Number: 1
Number of Pages: X, 340
Number of Illustrations: 31 b/w illustrations, 65 illustrations in colour
Topics: Protein Science