Overview
- Introduces protocols and their theoretical background relevant for a variety of experimental research in pre-mRNA splicing
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1126)
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Table of contents (28 protocols)
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Front Matter
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Methods Chapters
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Front Matter
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Keywords
About this book
Providing a guide to classical experimental approaches to decipher splicing mechanisms and experimental strategies that rely on novel multi-disciplinary approaches, Spliceosomal Pre-mRNA Splicing: Methods and Protocols describes the theory of alternative pre-mRNA splicing in seven introductory chapters and then introduces protocols and their theoretical background relevant for a variety of experimental research. These protocol chapters cover basic methods to detect splicing events, analyses of alternative pre-mRNA splicing in vitro and in vivo manipulation of splicing events and high-throughput and bioinformatic analyses of alternative splicing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, Spliceosomal Pre-mRNA Splicing: Methods and Protocols will aid newcomers and seasoned molecular biologists in understanding the fascinating world of alternative splicing with the ultimate goal of paving the way for many new discoveries to come.
Editors and Affiliations
Bibliographic Information
Book Title: Spliceosomal Pre-mRNA Splicing
Book Subtitle: Methods and Protocols
Editors: Klemens J. Hertel
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-980-2
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2014
Hardcover ISBN: 978-1-62703-979-6Published: 21 February 2014
Softcover ISBN: 978-1-4939-6044-6Published: 03 September 2016
eBook ISBN: 978-1-62703-980-2Published: 18 February 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 427
Number of Illustrations: 32 b/w illustrations, 34 illustrations in colour
Topics: Human Genetics