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Cereal Genomics

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Robert J. Henry

    1. Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, Australia

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    You can also search for this editor in PubMed   Google Scholar

  2. Agnelo Furtado

    1. Centre for Nutrition and Food Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Lismore, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Is a comprehensive resource for studying cereal genomes
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1099)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. DNA Extraction from Vegetative Tissue for Next-Generation Sequencing

    • Agnelo Furtado
    Pages 1-5

  3. DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples)

    • Chiaki Mutou, Katsunori Tanaka, Ryuji Ishikawa
    Pages 7-15

  4. RNA Extraction from Cereal Vegetative Tissue

    • Julie A. Pattemore
    Pages 17-21

  5. RNA Extraction from Developing or Mature Wheat Seeds

    • Agnelo Furtado
    Pages 23-28

  6. cDNA Library Preparation

    • Maarten Kooiker, Gang-Ping Xue
    Pages 29-40

  7. Preparation of High Molecular Weight gDNA and Bacterial Artificial Chromosome (BAC) Libraries in Plants

    • Siddanagouda S. Biradar, Xiaojun Nie, Kewei Feng, Song Weining
    Pages 41-63

  8. The Polymerase Chain Reaction (PCR): General Methods

    • Daniel L. E. Waters, Frances M. Shapter
    Pages 65-75

  9. Mutation and Mutation Screening

    • L. Slade Lee, Bradley J. Till, Helen Hill, Owen A. Huynh, Joanna Jankowicz-Cieslak
    Pages 77-95

  10. The Quantitative Real-Time Polymerase Chain Reaction for the Analysis of Plant Gene Expression

    • Timothy L. Fitzgerald, Richard B. McQualter
    Pages 97-115

  11. Cloning of DNA Fragments: Ligation Reactions in Agarose Gel

    • Agnelo Furtado
    Pages 117-121

  12. Rapid Cloning of Genes and Promoters for Functional Analyses

    • Peer M. Schenk
    Pages 123-132

  13. Genome Walking

    • Frances M. Shapter, Daniel L. E. Waters
    Pages 133-146

  14. Functional Analysis by Protein Biochemistry

    • Louis M. T. Bradbury
    Pages 147-158

  15. Genomic Southern Blot Analysis

    • Leigh Gebbie
    Pages 159-177

  16. Northern Hybridization: A Proficient Method for Detection of Small RNAs and MicroRNAs

    • Shazia Iram
    Pages 179-188

  17. Protein Blotting Protocol for Beginners

    • Lars A. Petrasovits
    Pages 189-199

  18. Genetic Transformation of Wheat via Particle Bombardment

    • Caroline A. Sparks, Huw D. Jones
    Pages 201-218

  19. Sorghum Genetic Transformation by Particle Bombardment

    • Guoquan Liu, Bradley C. Campbell, Ian D. Godwin
    Pages 219-234

  20. Genetic Transformation of Wheat via Agrobacterium-Mediated DNA Delivery

    • Caroline A. Sparks, Angela Doherty, Huw D. Jones
    Pages 235-250
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Keywords

About this book

​In Cereal Genomics: Methods and Protocols, expert researchers provides modern protocols for the analysis and manipulation of cereal genomes.  Techniques for isolation and analysis of DNA and RNA from both the vegetative tissues and from the more challenging seeds of cereals are described.  Tools for the isolation, characterization and functional analysis of cereal genes and their transcripts are detailed. Methods for molecular screening of cereals and for their genetic transformation are also covered. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cereal Genomics: Methods and Protocols provides a comprehensive resource for those studying cereal genomes.

Editors and Affiliations

  • Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, Australia

    Robert J. Henry

  • Centre for Nutrition and Food Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Lismore, Australia

    Agnelo Furtado

Bibliographic Information

  • Book Title: Cereal Genomics

  • Book Subtitle: Methods and Protocols

  • Editors: Robert J. Henry, Agnelo Furtado

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-715-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2014

  • Hardcover ISBN: 978-1-62703-714-3Published: 16 November 2013

  • Softcover ISBN: 978-1-4939-6039-2Published: 23 August 2016

  • eBook ISBN: 978-1-62703-715-0Published: 19 November 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 300

  • Number of Illustrations: 10 b/w illustrations, 29 illustrations in colour

  • Topics: Plant Sciences, Plant Genetics and Genomics, Transgenics

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