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Plant Metabolic Flux Analysis

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Martine Dieuaide-Noubhani

    1. INRA Bordeaux Université Bordeaux Segalen, Villenave d'Ornon, France

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    You can also search for this editor in PubMed   Google Scholar

  2. Ana Paula Alonso

    1. Department of Molecular Genetics, The Ohio State University, Columbus, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1090)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Application of Metabolic Flux Analysis to Plants

    • Martine Dieuaide-Noubhani, Ana Paula Alonso
    Pages 1-17

  3. Metabolic Network Reconstruction and Their Topological Analysis

    • Marie Beurton-Aimar, Tung Vu-Ngoc Nguyen, Sophie Colombié
    Pages 19-38

  4. Experimental Design and Acquistion of Labeling Data (Experimental Design and Data Acquisition for Isotopic Labeling Based Metabolic Flux Analysis)

    1. Front Matter

      Pages 39-39
      Download chapter PDF

    2. 14C Pulse Labeling to Estimate External Fluxes and Turnovers in Primary Metabolism

      • Katia Pianelli, Antoine Monier, Marie-Hélène Andrieu, Bertrand Beauvoit, Martine Dieuaide-Noubhani
      Pages 41-52

    3. Optimization of Steady-State 13C-Labeling Experiments for Metabolic Flux Analysis

      • Nicholas J. Kruger, Shyam K. Masakapalli, R. George Ratcliffe
      Pages 53-72

    4. Quantification of 13C Enrichments and Isotopomer Abundances for Metabolic Flux Analysis Using 1D NMR Spectroscopy

      • Shyam K. Masakapalli, R. George Ratcliffe, Thomas C. R. Williams
      Pages 73-86

    5. Analysis of Proteinogenic Amino Acid and Starch Labeling by 2D NMR

      • Quyen Truong, Jacqueline V. Shanks
      Pages 87-105

    6. Analysis of Kinetic Labeling of Amino Acids and Organic Acids by GC-MS

      • Wagner L. Araújo, Takayuki Tohge, Adriano Nunes-Nesi, Toshihiro Obata, Alisdair R. Fernie
      Pages 107-119

    7. Quantifying 13C-labeling in Free Sugars and Starch by GC-MS

      • Mohamed Koubaa, Brigitte Thomasset, Albrecht Roscher
      Pages 121-130

    8. Liquid Chromatography Tandem Mass Spectrometry for Measuring 13C-labeling in Intermediates of the Glycolysis and Pentose Phosphate Pathway

      • Jean-Christophe Cocuron, Ana Paula Alonso
      Pages 131-142

    9. In Vivo NMR for 13C metabolic Flux Analysis

      • Albrecht Roscher, Stéphanie Troufflard, Abdelghani Idrissi Taghki
      Pages 143-152

  5. From Labeling Data to Flux Maps (from the Labeling Data to a Metabolic Map)

    1. Front Matter

      Pages 153-153
      Download chapter PDF

    2. Steady-State and Instationary Modeling of Proteinogenic and Free Amino Acid Isotopomers for Flux Quantification

      • Yuting Zheng, Ganesh Sriram
      Pages 155-179

    3. Isotopically Nonstationary MFA (INST-MFA) of Autotrophic Metabolism

      • Lara J. Jazmin, John P. O’Grady, Fangfang Ma, Doug K. Allen, John A. Morgan, Jamey D. Young
      Pages 181-210

    4. Simulating Labeling to Estimate Kinetic Parameters for Flux Control Analysis

      • Amy Marshall-Colon, Neelanjan Sengupta, David Rhodes, John A. Morgan
      Pages 211-222

    5. High-Throughput Data Pipelines for Metabolic Flux Analysis in Plants

      • C. Hart Poskar, Jan Huege, Christian Krach, Yair Shachar-Hill, Björn H. Junker
      Pages 223-246

  6. Predicting Fluxes in Plants

    1. Front Matter

      Pages 247-247
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    2. Analysis of Enzyme Activities

      • Guillaume Ménard, Benoit Biais, Duyên Prodhomme, Patricia Ballias, Yves Gibon
      Pages 249-259

    3. Analytical Kinetic Modeling: A Practical Procedure

      • Gilles Curien, María L. Cárdenas, Athel Cornish-Bowden
      Pages 261-280
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Keywords

About this book

In Plant Metabolic Flux Analysis, expert researchers in the field provide detailed experimental procedures for each step of the flux quantification workflow. Steady state and dynamic modeling are considered, as well as recent developments for the reconstruction of metabolic networks and for a predictive modeling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical Plant Metabolic Flux Analysis, seeks to aid scientists in the further study of cutting-edge protocols and methodologies that are crucial to getting ahead in MFA.

Editors and Affiliations

  • INRA Bordeaux Université Bordeaux Segalen, Villenave d'Ornon, France

    Martine Dieuaide-Noubhani

  • Department of Molecular Genetics, The Ohio State University, Columbus, USA

    Ana Paula Alonso

Bibliographic Information

  • Book Title: Plant Metabolic Flux Analysis

  • Book Subtitle: Methods and Protocols

  • Editors: Martine Dieuaide-Noubhani, Ana Paula Alonso

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-688-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2014

  • Hardcover ISBN: 978-1-62703-687-0Published: 13 November 2013

  • Softcover ISBN: 978-1-4939-6050-7Published: 20 September 2016

  • eBook ISBN: 978-1-62703-688-7Published: 13 November 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 365

  • Number of Illustrations: 44 b/w illustrations, 31 illustrations in colour

  • Topics: Plant Sciences, Plant Biochemistry, Metabolomics

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