Editors:
- Explores the methods and applications in the expanding fluorescence field
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1076)
Buy it now
Buying options
Tax calculation will be finalised at checkout
Other ways to access
This is a preview of subscription content, log in via an institution to check for access.
Table of contents (36 protocols)
-
Front Matter
-
Steady-State Fluorescence Spectroscopy (SSFS)
-
Front Matter
-
-
Time-Resolved Fluorescence Spectroscopy (TRFS)
-
Front Matter
-
-
Fluorescent Probe Development (FPD)
-
Front Matter
-
About this book
Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results.
Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques.
Keywords
- Cuvette-based studies
- Fluorescence Fluctuation Spectroscopy
- Fluorescence Lifetime Imaging
- Fluorescence Recovery After Photobleaching
- Fluorescent Probe Development
- Förster Resonance Energy Transfer Imaging
- Green fluorescent protein (GFP)
- Single-Molecule Fluorescence Spectroscopy
- Steady-State Fluorescence Spectroscopy
- Time-Resolved Fluorescence Spectroscopy
- Biological Microscopy
Reviews
From the reviews:
“The book is illustrated with high-quality figures in both color and black and white. … This book offers techniques from internationally recognized experts. Both novice and established scientists will find it extremely useful.” (Omer Iqbal, Doody’s Book Reviews, March, 2014)Editors and Affiliations
-
KULeuven-Biomolecular Dynamics BioSCENTer, Leuven, Belgium
Yves Engelborghs
-
MicroSpectroscopy Centre, University of Wageningen Biochemistry, Wageningen, The Netherlands
Antonie J.W.G. Visser
Bibliographic Information
Book Title: Fluorescence Spectroscopy and Microscopy
Book Subtitle: Methods and Protocols
Editors: Yves Engelborghs, Antonie J.W.G. Visser
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-649-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2014
Hardcover ISBN: 978-1-62703-648-1Published: 10 October 2013
Softcover ISBN: 978-1-4939-6315-7Published: 23 August 2016
eBook ISBN: 978-1-62703-649-8Published: 10 October 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XXI, 816
Number of Illustrations: 73 b/w illustrations, 171 illustrations in colour
Topics: Imaging / Radiology, Biological Microscopy, Spectroscopy/Spectrometry