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  • © 2014

Arabidopsis Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1062)

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Table of contents (36 protocols)

  1. Front Matter

    Pages i-xvi
  2. Growing Arabidopsis

    1. Front Matter

      Pages 1-1
    2. Handling Arabidopsis Plants: Growth, Preservation of Seeds, Transformation, and Genetic Crosses

      • Luz Rivero, Randy Scholl, Nicholas Holomuzki, Deborah Crist, Erich Grotewold, Jelena Brkljacic
      Pages 3-25
    3. Using Arabidopsis-Related Model Species (ARMS): Growth, Genetic Transformation, and Comparative Genomics

      • Giorgia Batelli, Dong-Ha Oh, Matilde Paino D’Urzo, Francesco Orsini, Maheshi Dassanayake, Jian-Kang Zhu et al.
      Pages 27-51
    4. Growing Arabidopsis In Vitro: Cell Suspensions, In Vitro Culture, and Regeneration

      • Bronwyn J. Barkla, Rosario Vera-Estrella, Omar Pantoja
      Pages 53-62
  3. Arabidopsis Resources

    1. Front Matter

      Pages 63-63
    2. Arabidopsis Database and Stock Resources

      • Donghui Li, Kate Dreher, Emma Knee, Jelena Brkljacic, Erich Grotewold, Tanya Z. Berardini et al.
      Pages 65-96
    3. Bioinformatic Tools in Arabidopsis Research

      • Miguel de Lucas, Nicholas J. Provart, Siobhan M. Brady
      Pages 97-136
  4. Genetic Techniques

    1. Front Matter

      Pages 137-137
    2. Exploiting Natural Variation in Arabidopsis

      • Johanna A. Molenaar, Joost J. B. Keurentjes
      Pages 139-153
    3. Grafting in Arabidopsis

      • Katherine Bainbridge, Tom Bennett, Peter Crisp, Ottoline Leyser, Colin Turnbull
      Pages 155-163
    4. Agrobacterium tumefaciens-Mediated Transient Transformation of Arabidopsis thaliana Leaves

      • Silvina Mangano, Cintia Daniela Gonzalez, Silvana Petruccelli
      Pages 165-173
    5. iTILLING: Personalized Mutation Screening

      • Susan M. Bush, Patrick J. Krysan
      Pages 175-191
    6. Tailor-Made Mutations in Arabidopsis Using Zinc Finger Nucleases

      • Yiping Qi, Colby G. Starker, Feng Zhang, Nicholas J. Baltes, Daniel F. Voytas
      Pages 193-209
    7. The Use of Artificial MicroRNA Technology to Control Gene Expression in Arabidopsis thaliana

      • Andrew L. Eamens, Marcus McHale, Peter M. Waterhouse
      Pages 211-224
    8. Identification of EMS-Induced Causal Mutations in Arabidopsis thaliana by Next-Generation Sequencing

      • Naoyuki Uchida, Tomoaki Sakamoto, Masao Tasaka, Tetsuya Kurata
      Pages 259-270
    9. Arabidopsis Transformation with Large Bacterial Artificial Chromosomes

      • Jose M. Alonso, Anna N. Stepanova
      Pages 271-283
    10. Global DNA Methylation Analysis Using Methyl-Sensitive Amplification Polymorphism (MSAP)

      • Mahmoud W. Yaish, Mingsheng Peng, Steven J. Rothstein
      Pages 285-298

About this book

Arabidopsis Protocols, Third Edition compiles some of the most recent methodologies developed to exploit the Arabidopsis genome. These methodologies cover from the guided access to public resources, to genetic, cell biology, biochemical and physiological techniques, including both those that are widely used as well as those novel techniques likely to open up new avenues of knowledge in the future.  In addition, considering the recent unparalleled progress of the “omics” tools in Arabidopsis, leading experts have contributed sections on genome, transcriptome, proteome, metabolome and other whole-system approaches. Arabidopsis thaliana is acknowledged as the most important plant model system by the scientific community and Arabidopsis research has fundamentally influenced our understanding of the basic biology and ecology of plants. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Arabidopsis Protocols, Third Edition seeks to serve both experienced researchers and beginners with its detailed methodologies on this burgeoning scientific field. 

Editors and Affiliations

  • CSIC Centro Nacional de Biotecnología, Madrid, Spain

    Jose J. Sanchez-Serrano

  • Depto. Biologia de Plantas, CSIC Madrid Centro de Investigaciones Biologicas, Madrid, Spain

    Julio Salinas

Bibliographic Information

  • Book Title: Arabidopsis Protocols

  • Editors: Jose J. Sanchez-Serrano, Julio Salinas

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-580-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-62703-579-8Published: 21 September 2013

  • Softcover ISBN: 978-1-4939-6276-1Published: 23 August 2016

  • eBook ISBN: 978-1-62703-580-4Published: 21 September 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XVI, 726

  • Number of Illustrations: 32 b/w illustrations, 70 illustrations in colour

  • Topics: Plant Genetics and Genomics, Gene Expression

Buy it now

Buying options

eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 199.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access