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Microglia

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Bertrand Joseph

    1. , Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden

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    You can also search for this editor in PubMed   Google Scholar

  2. José Luis Venero

    1. , Department of Biochemistry, University of Seville, Seville, Spain

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Features tips on trouble shooting and avoiding known pitfalls
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1041)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xii
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  2. Overview of Microglia Biology

    1. Front Matter

      Pages 1-1
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    2. A Brief Overview of Multitalented Microglia

      • Bertrand Joseph, José Luis Venero
      Pages 3-8

  3. Isolation and Culture of Microglia

    1. Front Matter

      Pages 9-9
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    2. Cell Culturing of Human and Murine Microglia Cell Lines

      • Johanna Rodhe
      Pages 11-16

    3. Microglia Isolation from Adult Mouse Brain

      • Jae-Kyung Lee, Malú G. Tansey
      Pages 17-23

    4. Preparation of Primary Microglia Cultures from Postnatal Mouse and Rat Brains

      • Tomas Deierborg
      Pages 25-31

    5. Isolation of Murine Postnatal Brain Microglia for Phenotypic Characterization Using Magnetic Cell Separation Technology

      • Ashley S. Harms, Malú G. Tansey
      Pages 33-39

    6. Isolation and Culture of Adult Human Microglia Within Mixed Glial Cultures for Functional Experimentation and High-Content Analysis

      • Amy M. Smith, Hannah M. Gibbons, Claire Lill, Richard L. M. Faull, Mike Dragunow
      Pages 41-51

  4. Depletion and Transduction of Microglia

    1. Front Matter

      Pages 53-53
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    2. Depletion of Microglia from Primary Cellular Cultures

      • Lorena Pont-Lezica, Sabrina Colasse, Alain Bessis
      Pages 55-61

    3. Lentiviral Transduction of Cultured Microglia

      • Takahiro Masuda, Makoto Tsuda, Hidetoshi Tozaki-Saitoh, Kazuhide Inoue
      Pages 63-67

  5. Analysis of Microglial Cytokine Production

    1. Front Matter

      Pages 69-69
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    2. Microglial Activation: Measurement of Cytokines by Flow Cytometry

      • Deepak Kumar Kaushik, Anirban Basu
      Pages 71-82

    3. In Situ Hybridization of Cytokine mRNA Using Alkaline Phosphatase-Labelled Oligodeoxynucleotide Probes

      • Bettina Clausen, Christina Fenger, Bente Finsen
      Pages 83-91

    4. Use of Meso-Scale Discovery™ to Examine Cytokine Content in Microglia Cell Supernatant

      • Miguel A. Burguillos
      Pages 93-100

  6. Analysis of Microglia Activation

    1. Front Matter

      Pages 101-101
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    2. Analysis of Microglial Production of Reactive Oxygen and Nitrogen Species

      • Urte Neniskyte, Guy C. Brown
      Pages 103-111

    3. Quantification of Active Caspase-3 and Active Caspase-8 in Microglia Cells

      • Edel Kavanagh
      Pages 113-120

    4. Quantification of Microglial Phagocytosis by a Flow Cytometer-Based Assay

      • Refik Pul, Kandiyil Prajeeth Chittappen, Martin Stangel
      Pages 121-127
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Keywords

About this book

 Key discoveries concerning the different biological functions of microglia in health and disease have attracted scientists from various fields. In Microglia: Methods and Protocols, expert researchers in the field detail methods for selection of the key cellular, molecular and biochemical techniques that are used in studying the many and varied functions of this fascinating cell. These methods and techniques include microglia cell culture for studying microglia activation and functions, as well as their interaction with other cell types both in vitro and in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Microglia: Methods and Protocols is a useful resource for cell biologists, molecular biologists, immunologists, oncologist and neuroscientists.

Editors and Affiliations

  • , Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden

    Bertrand Joseph

  • , Department of Biochemistry, University of Seville, Seville, Spain

    José Luis Venero

Bibliographic Information

  • Book Title: Microglia

  • Book Subtitle: Methods and Protocols

  • Editors: Bertrand Joseph, José Luis Venero

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-520-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-519-4Published: 28 June 2013

  • Softcover ISBN: 978-1-4939-6051-4Published: 23 August 2016

  • eBook ISBN: 978-1-62703-520-0Published: 28 June 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 350

  • Number of Illustrations: 15 b/w illustrations, 50 illustrations in colour

  • Topics: Neurosciences, Neurobiology

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