Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1010)
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Table of contents (18 protocols)
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Front Matter
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Introduction for Trinucleotide Repeat Diseases
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Front Matter
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Analysis of Synaptic Plasticity
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Front Matter
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Targeting on ES Cells
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Front Matter
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Targeting on RNA
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Front Matter
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Trinucleotide Repeat Expansion and Detection
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Front Matter
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About this book
Two decades have passed since trinucleotide repeat expansion was first discovered in genes responsible for certain neurological diseases. Since then, new technologies have developed and innovative concepts have emerged, which may prove useful in devising therapeutic approaches to neurological diseases. Divided into six convenient sections, Trinucleotide Repeat Protocols, Second Edition covers a wide range of topics such as an overview of trinucleotide repeat diseases, synaptic plasticity, embryonic stem (ES) cell-related protocols with a focus on HD, RNA-related protocols, and analysis of epigenetic modification in fragile X syndrome. Â This edition focuses not only on direct analysis of trinucleotide repeat diseases but also on alternative approaches for the analysis of trinucleotide repeat diseases, with the hope that this will result in a better understanding of the mechanisms and future therapeutic prospects for treatment of these diseases. Written in the successful Methods in Molecular Biologyâ„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
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Authoritative and easily accessible, Trinucleotide Repeat Protocols, Second Edition seeks to serve researchers with its thorough methodologies on this expanding field.
Editors and Affiliations
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Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, USA
Yoshinori Kohwi, Cynthia T. McMurray
Bibliographic Information
Book Title: Trinucleotide Repeat Protocols
Editors: Yoshinori Kohwi, Cynthia T. McMurray
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-411-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2013
Hardcover ISBN: 978-1-62703-410-4Published: 11 June 2013
Softcover ISBN: 978-1-4939-5971-6Published: 23 August 2016
eBook ISBN: 978-1-62703-411-1Published: 12 June 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XIII, 296
Number of Illustrations: 40 b/w illustrations, 24 illustrations in colour
Topics: Molecular Medicine, Gene Expression