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  • © 2012

Nanoparticles in Biology and Medicine

Methods and Protocols

  • Supplies cutting-edge laboratory protocols involving nanoparticles and their applications
  • Includes methods on fabrication and synthesis, manufacturing, and characterization
  • Features expert tips and key implementation advice to guarantee successful results

Part of the book series: Methods in Molecular Biology (MIMB, volume 906)

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Softcover Book USD 119.99
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Hardcover Book USD 169.99
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Table of contents (43 protocols)

  1. Front Matter

    Pages i-xvii
  2. Noble Metal Nanoparticles: Synthesis and Applications

    1. Front Matter

      Pages 1-1
    2. Procedures for the Synthesis and Capping of Metal Nanoparticles

      • Claudia Gutiérrez-Wing, J. Jesús Velázquez-Salazar, Miguel José-Yacamán
      Pages 3-19
    3. Biosynthesis of Silver and Gold Nanoparticles Using Bacillus licheniformis

      • Muthu Irulappan Sriram, Kalimuthu Kalishwaralal, Sangiliyandi Gurunathan
      Pages 33-43
    4. Gold Nanoparticle Antibody Conjugates for Use in Competitive Lateral Flow Assays

      • Julian Bailes, Samantha Mayoss, Phil Teale, Mikhail Soloviev
      Pages 45-55
    5. Effect of Gold Nanoparticle Conjugation on the Activity and Stability of Functional Proteins

      • Julian Bailes, Sara Gazi, Rositsa Ivanova, Mikhail Soloviev
      Pages 89-99
  3. Semiconductor Nanoparticles: Synthesis and Applications

    1. Front Matter

      Pages 101-101
    2. Synthesis of Semiconductor Nanoparticles

      • Xianfeng Chen, Peter J. Dobson
      Pages 103-123
    3. Size-Selective Synthesis of Ultrasmall Hydrophilic CdSe Nanoparticles in Aqueous Solution at Room Temperature

      • Yeon-Su Park, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba
      Pages 125-141
    4. In Vivo, Site-Specific, Covalent Conjugation of Quantum Dots to Proteins via Split-Intein Splicing

      • Anna Charalambous, Maria Andreou, Ioanna Antoniades, Neophytos Christodoulou, Paris A. Skourides
      Pages 157-169
    5. Evaluating the Potential of Quantum Dots for In Vitro Biological Studies: Effects on Gene Expression Using Microarray Analysis

      • Babu R. Prasad, Enda O’Connell, Terry J. Smith, Valérie A. Gérard, Yurii K. Gun’ko, Yury Rochev
      Pages 171-183
    6. The Use of Quantum Dots for Immunochemistry Applications

      • Helena Montón, Mónica Roldán, Arben Merkoçi, Emma Rossinyol, Onofre Castell, Carme Nogués
      Pages 185-192
    7. Labeling and Imaging Mesenchymal Stem Cells with Quantum Dots

      • Maria C. Collins, Philip R. Gunst, Wayne E. Cascio, Alan P. Kypson, Barbara J. Muller-Borer
      Pages 199-210
  4. Magnetic Nanoparticles: Synthesis and Applications

    1. Front Matter

      Pages 219-219

About this book

The modern fascination with micro- and nano-sized materials can actually be traced back further to the 1960s and ‘70s when the first few reported attempts were made to use nanoparticles for controlled drug delivery.  In Nanoparticles in Biology and Medicine: Methods and Protocols, experts in the field present a wide range of methods for synthesis, surface modification, characterization, and application of nano-sized materials (nanoparticles) in life science and medical fields, mostly for drug delivery. The methods presented cover all stages of nanoparticle manufacturing, modification, analysis, and applications.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and cutting-edge, Nanoparticles in Biology and Medicine: Methods and Protocols will help the beginner become familiar with this fascinating field and will provide scientists at all levels of expertise with easy-to-follow practical advice needed to make, modify, and analyze nanoparticles of their choice and to use them in a wide range of biomedical and pharmaceutical applications, including functional protein studies, drug delivery, immunochemistry, imaging, and many others.

Reviews

From the book reviews:

“This is another of the series ‘Methods in Molecular Biology’ and covers various aspects of the synthesis, applications and characterization of nanoparticles. … this book makes a very useful source of information for those interested in how nanoparticles can be used for various imaging techniques and applications such as drug delivery. Those wishing to synthesise nanoparticles for these applications will also find it a useful guide with easy to follow methods and guidance.” (Bobbi Bradford, BTS Newsletter – The British Toxicology Society, Issue 43, Winter, 2013)

“This is a how-to-do-it manual on the applications of nanoparticles in modern medicine. … This book is of great value for anyone who is involved in the manufacturing, modification, analysis, or application of nanoparticles for use in medicine. … This volume represents a comprehensive and cutting-edge resource for investigators at all levels who have an interest in the application of nanoparticle technology to a wide range of uses in the biomedical and pharmaceutical fields … .” (Thomas L. Pazdernik, Doody’s Book Reviews, February, 2013)

“This book aims to cover the complete production process for such nanoparticles, including their synthesis, physicochemical characterization, and toxicity and efficacy evaluation. … the articles are clearly structured, and the protocols are easy to follow. … The index listing both keywords and abbreviations helps the novice researcher to find the desired protocol. … this book could be useful to researchers from a broad range of disciplines interested in protocols for methods outside their direct field of expertise.” (Eleonore Fröhlich, ChemMedChem, Issue 8, 2013)

Editors and Affiliations

  • School of Biological Sciences, Royal Holloway University of London, Egham, Surrey, United Kingdom

    Mikhail Soloviev

Bibliographic Information

  • Book Title: Nanoparticles in Biology and Medicine

  • Book Subtitle: Methods and Protocols

  • Editors: Mikhail Soloviev

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-953-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-952-5Published: 13 July 2012

  • Softcover ISBN: 978-1-4939-6247-1Published: 23 August 2016

  • eBook ISBN: 978-1-61779-953-2Published: 12 July 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 555

  • Topics: Pharmacology/Toxicology

Buy it now

Buying options

Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access