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  • © 2012

Ribozymes

Methods and Protocols

Editors:

  • Investigates mechanistic aspects of ribozymes catalysis
  • Provides step-by-step detial essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 848)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiv
  2. Introduction

    • Eric Westhof
    Pages 1-4
  3. Characterization of Hammerhead Ribozyme Reactions

    • Anne Kalweit, Rita Przybilski, Carsten Seehafer, Marcos de la Peña, Christian Hammann
    Pages 5-20
  4. Mechanistic Analysis of the Hepatitis Delta Virus (HDV) Ribozyme: Methods for RNA Preparation, Structure Mapping, Solvent Isotope Effects, and Co-transcriptional Cleavage

    • Durga M. Chadalavada, Andrea L. Cerrone-Szakal, Jennifer L. Wilcox, Nathan A. Siegfried, Philip C. Bevilacqua
    Pages 21-40
  5. Kinetic Characterization of Hairpin Ribozyme Variants

    • Bettina Appel, Thomas Marschall, Anne Strahl, Sabine Müller
    Pages 41-59
  6. Characterization of RNase P RNA Activity

    • Markus Gößringer, Dominik Helmecke, Roland K. Hartmann
    Pages 61-72
  7. Group I Intron Ribozymes

    • Henrik Nielsen
    Pages 73-89
  8. Mechanism and Distribution of glmS Ribozymes

    • Phillip J. McCown, Wade C. Winkler, Ronald R. Breaker
    Pages 113-129
  9. Structure-Based Search and In Vitro Analysis of Self-Cleaving Ribozymes

    • Randi M. Jimenez, Andrej Lupták
    Pages 131-143
  10. Discovery of RNA Motifs Using a Computational Pipeline that Allows Insertions in Paired Regions and Filtering of Candidate Sequences

    • Randi M. Jimenez, Ladislav Rampášek, Broňa Brejová, Tomáš Vinař, Andrej Lupták
    Pages 145-158
  11. Crystallographic Analysis of Small Ribozymes and Riboswitches

    • Geoffrey M. Lippa, Joseph A. Liberman, Jermaine L. Jenkins, Jolanta Krucinska, Mohammad Salim, Joseph E. Wedekind
    Pages 159-184
  12. Functional Dynamics of RNA Ribozymes Studied by NMR Spectroscopy

    • Boris Fürtig, Janina Buck, Christian Richter, Harald Schwalbe
    Pages 185-199
  13. Deoxyribozyme-Based, Semisynthetic Access to Stable Peptidyl-tRNAs Exemplified by tRNAVal Carrying a Macrolide Antibiotic Resistance Peptide

    • Dagmar Graber, Krista Trappl, Jessica Steger, Anna-Skrollan Geiermann, Lukas Rigger, Holger Moroder et al.
    Pages 201-213
  14. Single Molecule FRET Characterization of Large Ribozyme Folding

    • Lucia Cardo, Krishanthi S. Karunatilaka, David Rueda, Roland K. O. Sigel
    Pages 227-251
  15. Metal Ion–RNA Interactions Studied via Multinuclear NMR

    • Daniela Donghi, Roland K. O. Sigel
    Pages 253-273
  16. Analysis of Catalytic RNA Structure and Function by Nucleotide Analog Interference Mapping

    • Soumitra Basu, Mark J. Morris, Catherine Pazsint
    Pages 275-296
  17. In Vitro Selection of Metal Ion-Selective DNAzymes

    • Hannah E. Ihms, Yi Lu
    Pages 297-316
  18. Selecting Allosteric Ribozymes

    • Nicolas Piganeau
    Pages 317-328

About this book

Over the past decade, there have been a variety of fundamental insights into ribozymes which have changed the perspective on RNA catalysis. In, Ribozymes: Methods and Protocols, researchers in the field detail many of the protocols and techniques which are now commonly used to study individual ribozymes and the characterization of ribozymes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Ribozymes: Methods and Protocols seeks to aid scientists in further understanding the catalytically active RNAs .

Editors and Affiliations

  • Department of Chemistry, Universität Konstanz, Konstanz, Germany

    Jörg S. Hartig

Bibliographic Information

  • Book Title: Ribozymes

  • Book Subtitle: Methods and Protocols

  • Editors: Jörg S. Hartig

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-545-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-544-2Published: 09 February 2012

  • Softcover ISBN: 978-1-4939-5914-3Published: 23 August 2016

  • eBook ISBN: 978-1-61779-545-9Published: 08 February 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 480

  • Topics: Biochemistry, general

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access