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Protein NMR Techniques

  • Book
  • © 2012

Overview

Editors:
  1. Alexander Shekhtman

    1. University of Albany, State University of New York, Albany, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. David S. Burz

    1. University of Albany, State University of New York, Albany, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Comprehensive description of the latest innovations in the field of protein NMR
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 831)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. A Novel Bacterial Expression Method with Optimized Parameters for Very High Yield Production of Triple-Labeled Proteins

    • Victoria Murray, Yuefei Huang, Jianglei Chen, Jianjun Wang, Qianqian Li
    Pages 1-18

  3. Isotopic Labeling of Heterologous Proteins in the Yeast Pichia pastoris and Kluyveromyces lactis

    • Toshihiko Sugiki, Osamu Ichikawa, Mayumi Miyazawa-Onami, Ichio Shimada, Hideo Takahashi
    Pages 19-36

  4. Isotope Labeling in Insect Cells

    • Krishna Saxena, Arpana Dutta, Judith Klein-Seetharaman, Harald Schwalbe
    Pages 37-54

  5. Isotope Labeling in Mammalian Cells

    • Arpana Dutta, Krishna Saxena, Harald Schwalbe, Judith Klein-Seetharaman
    Pages 55-69

  6. Cell-Free Protein Production for NMR Studies

    • Mitsuhiro Takeda, Masatsune Kainosho
    Pages 71-84

  7. Cell-Free Membrane Protein Expression for Solid-State NMR

    • Alaa Abdine, Kyu-Ho Park, Dror E. Warschawski
    Pages 85-109

  8. Expression and Purification of Src-family Kinases for Solution NMR Studies

    • Andrea Piserchio, David Cowburn, Ranajeet Ghose
    Pages 111-131

  9. NMR Studies of Large Protein Systems

    • Shiou-Ru Tzeng, Ming-Tao Pai, Charalampos G. Kalodimos
    Pages 133-140

  10. Protein Dynamics by 15N Nuclear Magnetic Relaxation

    • Fabien Ferrage
    Pages 141-163

  11. Bacterial Production and Solution NMR Studies of a Viral Membrane Ion Channel

    • Jolyon K. Claridge, Jason R. Schnell
    Pages 165-179

  12. Preparation of the Modular Multi-Domain Protein RPA for Study by NMR Spectroscopy

    • Chris A. Brosey, Marie-Eve Chagot, Walter J. Chazin
    Pages 181-195

  13. NMR Studies of Protein–RNA Interactions

    • Carla A. Theimer, Nakesha L. Smith, May Khanna
    Pages 197-218

  14. Preparation and Optimization of Protein–DNA Complexes Suitable for Detailed NMR Studies

    • My D. Sam, Robert T. Clubb
    Pages 219-232

  15. NMR Studies of Protein–Ligand Interactions

    • Michael Goldflam, Teresa Tarragó, Margarida Gairí, Ernest Giralt
    Pages 233-259

  16. In-Cell NMR Spectroscopy in Escherichia coli

    • Kirsten E. Robinson, Patrick N. Reardon, Leonard D. Spicer
    Pages 261-277

  17. Deuterated Peptides and Proteins: Structure and Dynamics Studies by MAS Solid-State NMR

    • Bernd Reif
    Pages 279-301

  18. Solid-State NMR Spectroscopy of Protein Complexes

    • Shangjin Sun, Yun Han, Sivakumar Paramasivam, Si Yan, Amanda E. Siglin, John C. Williams et al.
    Pages 303-331

  19. Synthesis, Purification, and Characterization of Single Helix Membrane Peptides and Proteins for NMR Spectroscopy

    • Miki Itaya, Ian C. Brett, Steven O. Smith
    Pages 333-357

  20. Assignment of Backbone Resonances in a Eukaryotic Protein Kinase – ERK2 as a Representative Example

    • Andrea Piserchio, Kevin N. Dalby, Ranajeet Ghose
    Pages 359-368
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Keywords

About this book

The field of protein NMR spectroscopy has rapidly expanded into new areas of biochemistry, molecular biology and cell biology research that were impossible to study as recently as ten years ago. This third edition of Protein NMR Techniques, expands upon the previous editions with current, detailed authoritative but down-to-earth descriptions of new methodologies. These include techniques for NMR sample preparation, solution and solid state NMR methodologies and data processing. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Protein NMR Techniques,Third Edition, seeks to aid scientists in understanding the latest innovations in the field of protein NMR.

Editors and Affiliations

  • University of Albany, State University of New York, Albany, USA

    Alexander Shekhtman, David S. Burz

Bibliographic Information

  • Book Title: Protein NMR Techniques

  • Editors: Alexander Shekhtman, David S. Burz

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-480-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-479-7Published: 14 December 2011

  • Softcover ISBN: 978-1-4939-6195-5Published: 23 August 2016

  • eBook ISBN: 978-1-61779-480-3Published: 15 December 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XIV, 518

  • Topics: Protein Science, Spectroscopy/Spectrometry

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