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Chemical Proteomics

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Gerard Drewes

    1. CellZome AG, Heidelberg, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Marcus Bantscheff

    1. CellZome AG, Heidelberg, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Contributes to a wider application of chemical proteomics methods in biochemical and cell biological laboratories
  • Provides step-by-step detail essential for reprodicible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 803)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
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  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Mass Spectrometry-Based Chemoproteomic Approaches

      • Marcus Bantscheff
      Pages 3-13

    3. Chemical Proteomics in Drug Discovery

      • Gerard Drewes
      Pages 15-21

  3. Small Molecules and Probe Design

    1. Front Matter

      Pages 23-23
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    2. Compound Immobilization and Drug-Affinity Chromatography

      • Uwe Rix, Manuela Gridling, Giulio Superti-Furga
      Pages 25-38

    3. Affinity-Based Chemoproteomics with Small Molecule-Peptide Conjugates

      • Chaitanya Saxena
      Pages 39-54

    4. A Chemical Proteomic Probe for Detecting Dehydrogenases: Catechol Rhodanine

      • Xia Ge, Daniel S. Sem
      Pages 55-64

    5. Probing Proteomes with Benzophenone Photoprobes

      • Akira Kawamura, Doina M. Mihai
      Pages 65-75

    6. Biotinylated Probes for the Analysis of Protein Modification by Electrophiles

      • Simona G. Codreanu, Hye-Young H. Kim, Ned A. Porter, Daniel C. Liebler
      Pages 77-95

    7. Profiling of Methyltransferases and Other S-Adenosyl-l-Homocysteine-Binding Proteins by Capture Compound Mass Spectrometry

      • Thomas Lenz, Peter Poot, Elmar Weinhold, Mathias Dreger
      Pages 97-125

  4. Target Discovery and Target Validation

    1. Front Matter

      Pages 127-127
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    2. Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC

      • Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, Steven A. Carr
      Pages 129-140

    3. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric Mass Tags

      • Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, Marcus Bantscheff
      Pages 141-155

    4. Affinity-Based Profiling of Dehydrogenase Subproteomes

      • Xia Ge, Daniel S. Sem
      Pages 157-165

    5. Probing the Specificity of Protein–Protein Interactions by Quantitative Chemical Proteomics

      • Duangnapa Kovanich, Thin Thin Aye, Albert J. R. Heck, Arjen Scholten
      Pages 167-181

    6. Fluorescence-Based Proteasome Activity Profiling

      • Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, Celia R. Berkers
      Pages 183-204

    7. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein–Drug Interactions

      • Mathias Q. Müller, Andrea Sinz
      Pages 205-218

    8. Monitoring Ligand Modulation of Protein–Protein Interactions by Chemical Cross-Linking and High-Mass MALDI Mass Spectrometry

      • Natalia Gasilova, Alexis Nazabal
      Pages 219-229

    9. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

      • Amy Hye Won Jeon, Gerold Schmitt-Ulms
      Pages 231-246
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Keywords

About this book

The multidisciplinary science of chemical proteomics studies how small molecules of synthetic or natural origin bind to proteins and modulate their function.  In Chemical Proteomics: Methods and Protocols, expert researchers in the field provide key techniques to investigate chemical proteomics focusing on analytical strategies, how probes are generated, techniques for the discovery of small molecule targets and the probing of target function, and small molecule ligand and drug discovery.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Chemical Proteomics : Methods and Protocols seeks to provide methodologies that will contribute to a wider application of chemical proteomics methods in biochemical and cell biological laboratories.

Editors and Affiliations

  • CellZome AG, Heidelberg, Germany

    Gerard Drewes, Marcus Bantscheff

Bibliographic Information

  • Book Title: Chemical Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Gerard Drewes, Marcus Bantscheff

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-364-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-363-9Published: 08 November 2011

  • Softcover ISBN: 978-1-4939-5800-9Published: 23 August 2016

  • eBook ISBN: 978-1-61779-364-6Published: 09 November 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 313

  • Number of Illustrations: 61 b/w illustrations, 2 illustrations in colour

  • Topics: Protein Science, Proteomics

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