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Nanoproteomics

Methods and Protocols

  • Book
  • © 2011

Overview

Editors:
  1. Steven A. Toms

    1. , Department of Neurosurgery, Geisinger Health Systems, Danville, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Robert J. Weil

    1. Neurological Institute, Brain Tumor & Neuro-Oncology Center, The Cleveland Clinic, Cleveland, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Attempts to standardize and proliferate these key procedures in order to lead to wide scale adoption
  • Contains easy-to-follow methods and protocols, ready for the lab
  • Features expert tips and implementation advice to ensure successful results

Part of the book series: Methods in Molecular Biology (MIMB, volume 790)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Preliminary Sample Preparation

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. MALDI-TOF Serum Profiling Using Semiautomated Serum Peptide Capture with Magnetic Reversed Phase (C18) Beads

      • Jaco C. Knol, Connie R. Jimenez
      Pages 3-16

    3. Proteomics of Epithelial Lining Fluid Obtained by Bronchoscopic Microprobe Sampling

      • Lorenza Franciosi, Natalia Govorukhina, Nick ten Hacken, Dirkje Postma, Rainer Bischoff
      Pages 17-28

  3. Nanoscale Separation

    1. Front Matter

      Pages 29-29
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    2. Protein Identification Using Nano-HPLC-MS: ESI-MS and MALDI-MS Interfaces

      • Rui Vitorino, Jana Krenkova, Frantisek Foret, Pedro Domingues, Francisco Amado
      Pages 31-46

    3. Three-Dimensional Peptide Fractionation for Highly Sensitive Nanoscale LC-Based Shotgun Proteomic Analysis of Complex Protein Mixtures

      • Sricharan Bandhakavi, Todd W. Markowski, Hongwei Xie, Timothy J. Griffin
      Pages 47-56

    4. Nanospray Ion Mobility Mass Spectrometry of Selected High Mass Species

      • Iain Campuzano, Kevin Giles
      Pages 57-70

    5. Nanoelectrospray-MSn of Native and Permethylated Glycans

      • Christina Bleckmann, Hildegard Geyer, Rudolf Geyer
      Pages 71-85

    6. N-Linked Global Glycan Profiling by NanoLC Mass Spectrometry

      • Michael S. Bereman, David C. Muddiman
      Pages 87-97

    7. Applications of Nanoscale Liquid Chromatography Coupled to Tandem Mass Spectrometry in Quantitative Studies of Protein Expression, Protein–Protein Interaction, and Protein Phosphorylation

      • Metodi V. Metodiev
      Pages 99-113

    8. Nano LC–MS/MS: A Robust Setup for Proteomic Analysis

      • Marco Gaspari, Giovanni Cuda
      Pages 115-126

    9. Nanofluidic Devices for Rapid Continuous-Flow Bioseparation

      • Pan Mao, Jianping Fu
      Pages 127-140

    10. Quantifying Attomole Amounts of Proteins from Complex Samples by Nano-LC and Selected Reaction Monitoring

      • Thomas Fröhlich, Georg J. Arnold
      Pages 141-164

  4. Nanomaterials: Nanostructured Surfaces

    1. Front Matter

      Pages 165-165
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    2. A Sample Preparation Method for Gold Nanoparticle-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

      • Yen-Hsiu Lin, Wei-Lung Tseng
      Pages 167-172

    3. Nanostructured TiO2 Thin Films for Phosphoproteomics Studies with MALDI Mass Spectrometry

      • Federico Torta, Matteo Fusi, Carlo S. Casari, Andrea Li Bassi, Angela Bachi
      Pages 173-181

    4. Nanofilament Silicon for Matrix-Free Laser Desorption/Ionization Mass Spectrometry

      • Chia-Wen Tsao, Don L. DeVoe
      Pages 183-189

    5. Protein Nanoarrays for High-Resolution Patterning of Bacteria on Gold Surfaces

      • Cait Costello, Jan-Ulrich Kreft, Christopher M. Thomas, Paula M. Mendes
      Pages 191-200

  5. Nanomaterials: Nanoscale Tools for Proteomic Discovery

    1. Front Matter

      Pages 201-201
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Keywords

About this book

As two relatively new fields of study, proteomics and nanotechnology have developed in parallel with each other to allow an increased precision in the identification of post-translational protein modifications as well as to provide a more automated isolation and detection of rare proteins in both serum and tissues.  The Nanoproteomics: Methods and Protocols volume organizes and collects technical advances from leaders in the field to make laboratory protocols more readily available and understandable to those who are attempting to incorporate nanotechnologic techniques into their proteomic research. Conveniently divided into five sections, this detailed volume covers preliminary sample preparation, nanoscale fluidic devices and methods, nanostructured surfaces and nanomaterials, and nanoproteomic techniques to detect and understand protein and proteomic alterations specific to human pathology. Written in the highly successful series entitled Methods in Molecular Biology™, these chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols that are readily reproducible, and tips on troubleshooting and avoiding known pitfalls.

 

Convenient and authoritative, Nanoproteomics: Methods and Protocols offers key procedures that are culled from the laboratories of leaders in the field of nanoproteomics with the aim of helping researchers in their standardization and proliferation of protocols that will lead to a more wide scale adoption and smoother progress in this vital field.

Editors and Affiliations

  • , Department of Neurosurgery, Geisinger Health Systems, Danville, USA

    Steven A. Toms

  • Neurological Institute, Brain Tumor & Neuro-Oncology Center, The Cleveland Clinic, Cleveland, USA

    Robert J. Weil

Bibliographic Information

  • Book Title: Nanoproteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Steven A. Toms, Robert J. Weil

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-319-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-318-9Published: 23 September 2011

  • Softcover ISBN: 978-1-4939-5799-6Published: 23 August 2016

  • eBook ISBN: 978-1-61779-319-6Published: 27 October 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 322

  • Number of Illustrations: 34 b/w illustrations, 34 illustrations in colour

  • Topics: Protein Science, Proteomics

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