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  • © 2011

Cell Culture Techniques

  • Facilitates the study of adverse changes in the developing and mature brain alike
  • Features classic as well as innovative new approaches to neurotoxicologic testing
  • Aids in the reduction of the necessity of animal testing
  • Includes supplementary material: sn.pub/extras

Part of the book series: Neuromethods (NM, volume 56)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xiv
  2. Guidance on Good Cell Culture Practice (GCCP)

    • Anna Bal-Price, Sandra Coecke
    Pages 1-25
  3. Induced Pluripotent Stem Cells (iPSCs): An Emerging Model System for the Study of Human Neurotoxicology

    • M. Diana Neely, Andrew M. Tidball, Asad A. Aboud, Kevin C. Ess, Aaron B. Bowman
    Pages 27-61
  4. Neural Stem Cells

    • Roshan Tofighi, Christoffer Tamm, Michaela Moors, Wan Norhamidah Wan Ibrahim, Sandra Ceccatelli
    Pages 63-85
  5. Primary Cultures for Neurotoxicity Testing

    • Carme Solà, Rosa Cristòfol, Cristina Suñol, Coral Sanfeliu
    Pages 87-103
  6. Cell Culture to Investigate Neurotoxicity and Neurodegeneration Utilizing Caenorhabditis elegans

    • Michelle L. Tucci, Guy A. Caldwell, Kim A. Caldwell
    Pages 129-143
  7. Modelling the Blood–Brain Barrier

    • Marie-Pierre Dehouck, Elodie Vandenhaute, Lucie Dehouck, Emmanuel Sevin, Anne-Marie Lenfant, Yannick Delplace et al.
    Pages 145-160
  8. Introducing Cloned Genes into Cultured Neurons Providing Novel In vitro Models for Neuropathology and Neurotoxicity Studies

    • Marcelo Farina, Jordi Berenguer, Sebastián Pons, João Batista Teixeira da Rocha, Michael Aschner
    Pages 185-222
  9. P19 Embryonic Carcinoma Cell Line: A Model To Study Gene–Environment Interactions

    • Joseph Bressler, Cliona O’Driscoll, Cathleen Marshall, Walter Kaufmann
    Pages 223-240
  10. Signal Transduction and Neurotoxicity: What Can We Learn from Experimental Culture Systems?

    • Lucio G. Costa, Gennaro Giordano, Marina Guizzetti
    Pages 241-254
  11. The Use of Differentiating N2a and C6 Cell Lines for Studies of Organophosphate Toxicity

    • Alan J. Hargreaves, Magda Sachana, John Flaskos
    Pages 269-291
  12. Inflammation and Reactive Oxygen/Nitrogen Species in Glial/Neuronal Cultures

    • Jonas J. Neher, Guy C. Brown, Agnieszka Kinsner-Ovaskainen, Anna Bal-Price
    Pages 331-347
  13. Neuronal Oxidative Injury and Biomarkers of Lipid Peroxidation

    • Dejan Milatovic, Michael Aschner
    Pages 349-363
  14. Analysis of Protein Targets by Oxidative Stress Using the OxyBlot and Biotin–Avidin-Capture Methodology

    • Jeannette N. Stankowski, Simona G. Codreanu, Daniel C. Liebler, BethAnn McLaughlin
    Pages 365-381

About this book

Societal, ethical, and cost-related issues, not to mention the need for sound scientific methods, have led to new and refined methods for the evaluation of health risks associated with neurotoxic compounds, relevant and predictive of exposure, relatively inexpensive, and ideally amenable to high throughput analysis and a reduction in animal use.  Cell Culture Techniques presents thorough traditional chapters, such as those on various cell culture methods that have evolved over the years, as well as innovative approaches to neurotoxicologic testing.  Accordingly, this detailed volume describes how stem cells, computational biology, and other novel powerful methods can now be applied to address the challenges of neurotoxic testing.  As part of the Neuromethods series, this work provides the kind of intensive description and implementation advice that is crucial for getting optimal results in the laboratory.

 

Practical and authoritative, Cell Culture Techniques serves both the novice and the experienced neurotoxicologist by inspiring the further development of mechanistically-driven, cost-effective, high throughput series of tests needed to meet the many contemporary challenges.

Editors and Affiliations

  • , Department of Pediatrics, Vanderbilt University Medical Center, Nashville, USA

    Michael Aschner

  • Institut d’Investigacions Biomèdiques, Neurochemistry & Neuropharmacology, CSIC, Barcelona, Spain

    Cristina Suñol

  • Inst. for Health & Consumer Protection, ECVAM In-Vitro Methods Unit, European Commission JRC, Ispra, Italy

    Anna Bal-Price

Bibliographic Information

  • Book Title: Cell Culture Techniques

  • Editors: Michael Aschner, Cristina Suñol, Anna Bal-Price

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-61779-077-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-076-8Published: 08 March 2011

  • Softcover ISBN: 978-1-4939-5813-9Published: 23 August 2016

  • eBook ISBN: 978-1-61779-077-5Published: 01 March 2011

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XIV, 497

  • Topics: Neurosciences, Neurochemistry

Buy it now

Buying options

eBook USD 139.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 179.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access